In vitro three-dimensional cell cultures for bone sarcomas

Muñoz-García, Javier; Jubelin, Camille; Loussouarn, Aurélie; Goumard, Matisse; Griscom, Laurent; Renodon-Cornière, Axelle; Heymann, Marie-Françoise; Heymann, Dominique

doi:10.1016/j.jbo.2021.100379

Cited by 17 publications

(16 citation statements)

References 138 publications

(175 reference statements)

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“…Cocultured ES and mesenchymal stromal cells (MSCs) showed mutually stimulation of proliferation and altered responses to various inhibitors (123). Thus, the high resistance of ES to chemotherapy agents seems in part due to their particular MSCs origin but also to its complex TME (124). The coculture of these ES spheroids in the bone matrix tissue simulated typical tumor characteristics, including the induction of a hypoxic and glycolytic phenotype and the triggering of angiogenesis.…”

Section: Discussion: Physiological Chemoresistance Of Sclc and Esmentioning

confidence: 99%

Comparative characteristics of small cell lung cancer and Ewing’s sarcoma: a narrative review

Hamilton¹

2022

Transl Lung Cancer Res

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Background and Objective: Small cell lung cancer (SCLC) and Ewing's sarcoma (ES) at the disseminated stage are not amenable to therapy and have a dismal prognosis with low survival rates. Despite representing different tumor entities, treatment for both malignancies relies on cytotoxic chemotherapy that has not considerably changed for the past decades. The genomic background has been extensively studied and found to comprise inactivation of p53 and RB1 in case of SCLC and EWSR1/FLI1 rearrangement in case of ES resulting in aggressive tumors in adults with heavy tobacco consumption and as bone tumor in juveniles, respectively. New therapeutic modalities are urgently needed to improve the outcomes of both tumor entities, especially in patients with metastatic disease or recurrences. This review summarizes the common cell biologic and clinical characteristics of difficult-to-treat SCLC and ES and discusses their refractoriness and options to improve the therapeutic efficacy.

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Section: Discussion: Physiological Chemoresistance Of Sclc and Esmentioning

confidence: 99%

Comparative characteristics of small cell lung cancer and Ewing’s sarcoma: a narrative review

Hamilton¹

2022

Transl Lung Cancer Res

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“…To test the applicability of the 3D model in cell/surface interaction studies, a model was produced using the liquid overlay methodology. These spheres presented a diameter in a range very common for three-dimensional models found in the literature [ 27 , 28 , 29 ]. The tendency towards diameters between 300 µm and 400 µm can be explained by the fact that, in the human body, the distance of a cell from the nearest capillary is rarely greater than 200 µm [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Osteosphere Model to Evaluate Cell–Surface Interactions of Implantable Biomaterials

et al. 2021

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Successful biomaterials for bone tissue therapy must present different biocompatible properties, such as the ability to stimulate the migration and proliferation of osteogenic cells on the implantable surface, to increase attachment and avoid the risks of implant movement after surgery. The present work investigates the applicability of a three-dimensional (3D) model of bone cells (osteospheres) in the evaluation of osteoconductive properties of different implant surfaces. Three different titanium surface treatments were tested: machined (MA), sandblasting and acid etching (BE), and Hydroxyapatite coating by plasma spray (PSHA). The surfaces were characterized by Scanning Electron Microscopy (SEM) and atomic force microscopy (AFM), confirming that they present very distinct roughness. After seeding the osteospheres, cell–surface interactions were studied in relation to cell proliferation, migration, and spreading. The results show that BE surfaces present higher densities of cells, leaving the aggregates towards than titanium surfaces, providing more evidence of migration. The PSHA surface presented the lowest performance in all analyses. The results indicate that the 3D model allows the focal analysis of an in vitro cell/surfaces interaction of cells and surfaces. Moreover, by demonstrating the agreement with the clinical data observed in the literature, they suggest a potential use as a predictive preclinical tool for investigating osteoconductive properties of novel biomaterials for bone therapy.

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“…As stated above, the spatial modelization of cancer models is needed to integrate entirely the extra-cellular and cellular microenvironment. [53,54] For this final purpose, the next step was to introduce type I collagen for 3D spatialization of bone scaffold and create spheroids (described in Figure 3A and shown in Figure 3D). The final step to developing further the concept of bone matrix was the use of a more complex bone-like scaffold adding to collagen chitosan.…”

Section: D Osteosarcoma Models' Bioengineering Integrating Spatial An...mentioning

confidence: 99%

“…Several unstained cells (red arrows on Figure 4D) were spread in the scaffold and beside macrophages (black arrows on Figure 4D) or farther away, recreating cellular and extra-cellular microenvironmental components of OS more physiologically similar to native tumor tissue and rarely reproduced in recent OS models. [22,23,54]…”

Section: Complex Bone Scaffold Engineering Provides a 3d Microenviron...mentioning

confidence: 99%

Engineering Novel 3D Models to Recreate High‐Grade Osteosarcoma and its Immune and Extracellular Matrix Microenvironment

Pierrevelcin

Flacher

Mueller

et al. 2022

Adv Healthcare Materials

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Osteosarcoma (OS) is the most common primary bone cancer, where the overall 5-year surviving rate is below 20% in resistant forms. Accelerating cures for those poor outcome patients remains a challenge. Nevertheless, several studies of agents targeting abnormal cancerous pathways have yielded disappointing results when translated into clinic because of the lack of accurate OS preclinical modeling. So, any effort to design preclinical drug testing may consider all inter-, intra-, and extra-tumoral heterogeneities throughout models mimicking extracellular and immune microenvironment. Therefore, the bioengineering of patient-derived models reproducing the OS heterogeneity, the interaction with tumor-associated macrophages (TAMs), and the modulation of oxygen concentrations additionally to recreation of bone scaffold is proposed here. Eight 2D preclinical models mimicking several OS clinical situations and their TAMs in hypoxic conditions are developed first and, subsequently, the paired 3D models faithfully preserving histological and biological characteristics are generated. It is possible to shape reproducibly M2-like macrophages cultured with all OS patient-derived cell lines in both dimensions. The final 3D models pooling all heterogeneity features are providing accurate proliferation and migration data to understand the mechanisms involved in OS and immune cells/biomatrix interactions and sustained such that engineered 3D preclinical systems will improve personalized medicine.

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In vitro three-dimensional cell cultures for bone sarcomas

Cited by 17 publications

References 138 publications

Comparative characteristics of small cell lung cancer and Ewing’s sarcoma: a narrative review

Comparative characteristics of small cell lung cancer and Ewing’s sarcoma: a narrative review

Osteosphere Model to Evaluate Cell–Surface Interactions of Implantable Biomaterials

Engineering Novel 3D Models to Recreate High‐Grade Osteosarcoma and its Immune and Extracellular Matrix Microenvironment

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