Scaffold Design for Tissue Engineering

Chen, Guoping; Ushida, Takashi; Tateishi, Tetsuya

doi:10.1002/1616-5195(20020201)2:2<67::aid-mabi67>3.0.co;2-f

Cited by 580 publications

(249 citation statements)

References 32 publications

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“…Porous 3D scaffolds have been used extensively for skin tissue engineering [16]. Ideal scaffolds should have high cell seeding efficiency, promote fast cell attachment to the scaffolds and facilitate uniform spatial distribution of cells and extracellular matrix throughout the scaffolds [17]. It has been reported that a homogeneous spatial distribution of cells influences cell density in the engineered tissue constructs, which in turn impacts the kinetics of cell proliferation and extracellular matrix deposition and affects the functionality of engineered tissue [18].…”

Section: Discussionsupporting

confidence: 42%

PLLA–collagen and PLLA–gelatin hybrid scaffolds with funnel-like porous structure for skin tissue engineering

Naoki

et al. 2012

Science and Technology of Advanced Materials

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In skin tissue engineering, a three-dimensional porous scaffold is necessary to support cell adhesion and proliferation and to guide cells moving into the repair area in the wound healing process. Structurally, the porous scaffold should have an open and interconnected porous architecture to facilitate homogenous cell distribution. Moreover, the scaffolds should be mechanically strong to protect deformation during the formation of new skin. In this study, the hybrid scaffolds were prepared by forming funnel-like collagen or gelatin sponge on a woven poly(l-lactic acid) (PLLA) mesh. The hybrid scaffolds combined the advantages of both collagen or gelatin (good cell-interactions) and PLLA mesh (high mechanical strength). The hybrid scaffolds were used to culture dermal fibroblasts for dermal tissue engineering. The funnel-like porous structure promoted homogeneous cell distribution and extracellular matrix production. The PLLA mesh reinforced the scaffold to avoid deformation. Subcutaneous implantation showed that the PLLA-collagen and PLLA-gelatin scaffolds promoted the regeneration of dermal tissue and epidermis and reduced contraction during the formation of new tissue. These results indicate that funnel-like hybrid scaffolds can be used for skin tissue regeneration.

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Section: Discussionsupporting

confidence: 42%

PLLA–collagen and PLLA–gelatin hybrid scaffolds with funnel-like porous structure for skin tissue engineering

Naoki

et al. 2012

Science and Technology of Advanced Materials

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“…Moreover, for tissue engineering, previous studies have demonstrated that high mechanical strength is important for biodegradable polymer scaffolds (Hoerstrup et al, 2000;Shinoka et al, 1995;Shinoka et al, 1998;Stock et al, 2000) or allogenic acellular matrix scaffolds (Steinhoff et al, 2000). Major disadvantages of biodegradable polymer scaffolds include the requirement of reconstruction of the natural three-dimensional structures, and the absence of important ECM proteins in the synthetic polymers (Cheresh et al, 1989;Joshi et al, 1993). Thus, acellularized xenogenic tissues which maintain natural 3-dimensional structures are a promising alternative to biodegradable polymer scaffolds.…”

Section: Discussionsupporting

confidence: 41%

“…Pore size, pore number, and surface area are widely recognized as important parameters for scaffolds used in tissue engineering. Other architectural features such as pore shape and pore wall morphology of the scaffold material have also been suggested as important for cell seeding, migration, growth, and new tissue formation in three dimensions (Chen et al, 2002;Yang et al, 2001). In choosing appropriate material as the scaffold for use in , and Feng-Huei Lin corneal tissue engineering, the effects of corneal cell proliferation and migration on the material need to be considered in advance (Orwin and Hubel, 2000).…”

Section: Introductionmentioning

confidence: 41%

A new fish scale-derived scaffold for corneal regeneration

Lin

Ritch²,

Lin³

et al. 2010

eCM

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The purpose of this study is to develop a novel scaffold, derived from fish scales, as an alternative functional material with sufficient mechanical strength for corneal regenerative applications. Fish scales, which are usually considered as marine wastes, were acellularized, decalcified and fabricated into collagen scaffolds. The microstructure of the acellularized scaffold was imaged by scanning electron microscopy (SEM). The acellularization and decalcification treatments did not affect the naturally 3-dimentional, highly centrally-oriented micropatterned structure of the material. To assess the cytocompatibility of the scaffold with corneal cells, rabbit corneal cells were cultured on the scaffold and examined under SEM and confocal microscopy at different time periods. Rapid cell proliferation and migration on the scaffold were observed under SEM and confocal microscopy. The highly centrallyoriented micropatterned structure of the scaffold was beneficial for efficient nutrient and oxygen supply to the cells cultured in the three-dimensional matrices, and therefore it is useful for high-density cell seeding and spreading. Collectively, we demonstrate the superior cellular conductivity of the newly developed material. We provide evidences for the feasibility of the scaffold as a template for corneal cells growth and migration, and thus the fish scale-derived scaffold can be developed as a promising material for tissue-engineering of cornea.

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“…Different cell types were tested in TE protocols. To understand the importance of scaffolds, it is useful to consider studies that provide evidence of the ability of some biomaterial scaffolds to directly control stem cell differentiation (Chen et al 2002). In fact, the choice of appropriate scaffold is much more complex than the choice of cell type and depends on many factors.…”

Section: Introductionsupporting

confidence: 39%

The effects of synthetic and natural scaffolds on viability and proliferation of adipose-derived stem cells

Ghiasi

Kalhor

Qomi

et al. 2015

Frontiers in Life Science

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This study presents a comparative assessment of adipose-derived stem cell (ADSCs) proliferation rates and their viability on five different scaffolds. Five different biomaterial scaffolds were prepared: alginate, poly lactic-co-glycolic acid, fibrin glue, inactive platelet-rich plasma, and active plateletrich plasma (APRP). Stem cells were isolated from human adipose tissue. Flow cytometry analysis was performed. Specifically, adipogenesis/osteogenesis/chondrogenesis-associated genes expression was analyzed by real-time polymerase chain reaction. These cells were seeded in the prepared scaffolds. After 14 days, the proliferation and viability of MSCs were evaluated using an MTT assay. Also, stemness genes expression was analyzed with the reverse transcriptasepolymerase chain reaction (RT-PCR) method. In addition, the DNA content assay was also performed. The obtained results showed a significant difference between cell proliferation and viability of different scaffolds. APRP and alginate were shown to be the most and least suitable scaffolds in terms of enhancing cell proliferation and maintaining cell viability respectively (p < .05). RT-PCR reactions demonstrated the expression of the various stemness-related markers (Nanog, Octamer4A, and Sox2) when ADSC cells were grown separately on the five different scaffolds. Our study indicates that compared with the scaffolds, APRP could be the best scaffold for support of ADSC proliferation. ARTICLE HISTORY

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Scaffold Design for Tissue Engineering

Cited by 580 publications

References 32 publications

PLLA–collagen and PLLA–gelatin hybrid scaffolds with funnel-like porous structure for skin tissue engineering

PLLA–collagen and PLLA–gelatin hybrid scaffolds with funnel-like porous structure for skin tissue engineering

A new fish scale-derived scaffold for corneal regeneration

The effects of synthetic and natural scaffolds on viability and proliferation of adipose-derived stem cells

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