Sca1+ mesenchymal stromal cells inhibit graft-versus-host disease in mice after bone marrow transplantation

Fan, Wen; Zhang, Huijie; Chen, Yaozhen; Yue, Qiaohong; Liu, Zheng; Zhang, Qingping; Chen, Xiaopeng; Li, Na; Xin, Jiajia; Wang, Shaobin; Zhang, Xianqing; Xu, Xueqing; Hu, Xingbin

doi:10.1016/j.intimp.2015.03.001

Cited by 8 publications

(17 citation statements)

References 30 publications

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“…+ MSCs A bone-related MSC mixture and Sca1 + MSCs were isolated using a previously described method (Wen et al, 2015;Chen et al, 2016aChen et al, , 2016b. Tibias and femurs were excised from mice of 8-12 weeks old.…”

Section: Preparation Of Mscs and Sca1mentioning

confidence: 99%

“…At P < 0.05, the difference was considered statistically significant. (Wen et al, 2015;Chen et al, 2016aChen et al, , 2016b + cells using the transwell system ( Figures 1D-1F). Taken together, these data suggest that Sca1 + MSCs promoted DC commitment and that cell-to-cell contact was needed for the promotion.…”

Section: Statistical Analysesmentioning

confidence: 99%

“…Sca1 is one of the MSC markers (Morikawa et al, 2009). Many markers were used to identify in situ and/ or to isolate MSCs in mice, such as CD29, CD51, CD73, CD90, CD105, CD106, CD135, C-KIT, nestin, PDGFRα, and Sca1, but the lack of specific markers impedes identification of MSC functions (Galderisi and Giordano, 2014 (Wen et al, 2015;Chen et al, 2016aChen et al, , 2016b. Undoubtedly, niche components are instrumental to HSC self-renewal and differentiation, but in terms of lineage commitment, there are limited data available.…”

Section: Introductionmentioning

confidence: 99%

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The Sca1+ mesenchymal stromal subpopulation promotesdendritic cell commitment in the niche

Li¹,

Yin²,

Zhang³

et al. 2017

Turk J Biol

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IntroductionDendritic cells (DCs) are essential mediators in innate and adaptive immune responses. Except for Langerhans cells, DCs are derived from hematopoietic stem cells (HSCs) (Belz and Nutt, 2012;Satpathy et al., 2012;Seillet and Belz, 2013). Evidence has shown that self-renewing or the differentiation of HSCs, including DC commitment, mainly occurs in the bone marrow, with a high probability of being regulated by the hematopoietic microenvironment (niche) (Schraml et al., 2013). However, little is known about how the endosteal niche regulates the development of DC progenitors in bone marrow. Mesenchymal stromal cells (MSCs) are the most important components in the niche (Frenette et al., 2013). Recently, Jiang et al. (2005) reported that MSCs inhibited DC differentiation and maturation (Spaggiari et al., 2009;Zhang et al., 2009;Lai et al., 2010), as well as DC effector properties, including antigen processing and presentation to T cells (Chiesa et al., 2011).MSCs are actually heterogenic mixtures. A recent study reported that different subpopulations of MSCs served as unclassical niche components in bone marrow, such as CXCL12-abundant reticular (CAR) cells (Sugiyama et al., 2006;Ding and Morrison, 2013), nestin + cells (Mendez-Ferrer et al., 2010), Mx-cre + cells (Park et al., 2012), leptin + receptor-expressing mesenchymal cells (Ding et al., 2012), adipocytes (Naveiras et al., 2009), CD146 + cells, CD166 + cells (Hudson et al., 2011), and CD271 + cells (Mabuchi et al., 2013). Sca1 is one of the MSC markers (Morikawa et al., 2009). Many markers were used to identify in situ and/ or to isolate MSCs in mice, such as CD29, CD51, CD73, CD90, CD105, CD106, CD135, C-KIT, nestin, PDGFRα, and Sca1, but the lack of specific markers impedes identification of MSC functions (Galderisi and Giordano, 2014). Previously we enzymatically dissociated bone and separated the Sca1 + MSCs from the MSC mixture. Previously we also checked the purity of Sca1 + MSCs after isolation. Sca1 + MSCs were more than 80%, while CD45 + ,

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Section: Preparation Of Mscs and Sca1mentioning

confidence: 99%

Section: Statistical Analysesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Sca1+ mesenchymal stromal subpopulation promotesdendritic cell commitment in the niche

Li¹,

Yin²,

Zhang³

et al. 2017

Turk J Biol

Self Cite

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“…MSCs were isolated as previously described (Wen et al, 2015). The bone fragments were digested with collagenase type I (3 mg/mL, Sigma, Ronkonkoma, NY, USA) for 60 min at 37 °C.…”

Section: Bone Associated Mscsmentioning

confidence: 99%

“…Ablation of either the osteoblasts (Visnjic et al, 2001(Visnjic et al, , 2004, the CAR cells (Omatsu et al,2010) or the nestin + MSCs (Méndez-Ferrer et al, 2010), using lineage specific Cre-inducible systems, resulted in HSC reduction and relocation in BM. Recently, we found that stem cell antigen-1 (Sca-1) positive cells were one subpopulation of MSCs that participated in the self-renewal and differentiation of HSCs in adult mice (Wen et al, 2015). Nonetheless, further confirmatory functional studies of Sca-1 + MSCs in modulation of hematopoiesis are required.…”

Section: Introductionmentioning

confidence: 99%

The Sca-1+ mesenchymal stromal cells modulate macrophage commitment and function

Chen¹,

Chen²,

Yin³

et al. 2016

Turk J Biol

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Sca‐1⁺mesenchymal stromal cells inhibit splenic marginal zone B lymphocytes commitment through Caspase‐3

Chen

Yang

Zhang

et al. 2016

Cell Biology International

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Mesenchymal stromal cells (MSCs) have been characterized as an important component of hematopoietic niche, which are capable of modulating the immune system through interaction with a wide range of immune cells. Marginal zone B cells, one main type of mature B lymphocytes, play a central role in eliciting antibody response against pathogens. However, how MSCs and its subpopulations regulate marginal zone B cells commitment is unknown yet. In this study, we assessed the contribution of Sca-1(+) MSCs on marginal zone B cells commitment. Our results showed that Sca-1(+) MSCs inhibit the commitment of marginal zone B lymphocytes. The inhibition was exerted through lowered Caspase-3 expression. Furthermore, we found marginal zone B lymphocytes in spleen of Caspase-3 knockout mice decreased and Caspase-3 knockout Sca-1(+) MSCs accounted for the MZB lymphocytes decrease. In conclusion, our investigation provided clues about Sca-1(+) MSCs regulation on the commitment of marginal zone B cells through Caspase-3 gene.

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Sca1+ mesenchymal stromal cells inhibit graft-versus-host disease in mice after bone marrow transplantation

Cited by 8 publications

References 30 publications

The Sca1+ mesenchymal stromal subpopulation promotesdendritic cell commitment in the niche

The Sca1+ mesenchymal stromal subpopulation promotesdendritic cell commitment in the niche

The Sca-1+ mesenchymal stromal cells modulate macrophage commitment and function

Sca‐1⁺mesenchymal stromal cells inhibit splenic marginal zone B lymphocytes commitment through Caspase‐3

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Sca1+ mesenchymal stromal cells inhibit graft-versus-host disease in mice after bone marrow transplantation

Cited by 8 publications

References 30 publications

The Sca1+ mesenchymal stromal subpopulation promotesdendritic cell commitment in the niche

The Sca1+ mesenchymal stromal subpopulation promotesdendritic cell commitment in the niche

The Sca-1+ mesenchymal stromal cells modulate macrophage commitment and function

Sca‐1+mesenchymal stromal cells inhibit splenic marginal zone B lymphocytes commitment through Caspase‐3

Contact Info

Product

Resources

About

Sca‐1⁺mesenchymal stromal cells inhibit splenic marginal zone B lymphocytes commitment through Caspase‐3