Sca‐1⁺mesenchymal stromal cells inhibit splenic marginal zone B lymphocytes commitment through Caspase‐3

Abstract

Mesenchymal stromal cells (MSCs) have been characterized as an important component of hematopoietic niche, which are capable of modulating the immune system through interaction with a wide range of immune cells. Marginal zone B cells, one main type of mature B lymphocytes, play a central role in eliciting antibody response against pathogens. However, how MSCs and its subpopulations regulate marginal zone B cells commitment is unknown yet. In this study, we assessed the contribution of Sca-1(+) MSCs on marginal zone B cells commitment. Our results showed that Sca-1(+) MSCs inhibit the commitment of marginal zone B lymphocytes. The inhibition was exerted through lowered Caspase-3 expression. Furthermore, we found marginal zone B lymphocytes in spleen of Caspase-3 knockout mice decreased and Caspase-3 knockout Sca-1(+) MSCs accounted for the MZB lymphocytes decrease. In conclusion, our investigation provided clues about Sca-1(+) MSCs regulation on the commitment of marginal zone B cells through Caspase-3 gene.

Mesenchymal stromal cells can be applied to red blood cells storage as a kind of cellular additive

“…MSCs were identified by PE anti-mouse CD44 (IM7, BD, U.S.A.), PE anti-mouse CD51 (RMV-7, eBioscience, San Diego, U.S.A.), APC anti-mouse CD90 (OX-7, BD, U.S.A.), APC anti-mouse CD105 (MJ7/18, BioLegend, California, U.S.A.), APC anti-mouse CD45 (30-F11, BioLegend, California, U.S.A.), and APC anti-mouse Ter119 (17-5921, eBioscience, San Diego, U.S.A.). The FACS plots showed that the surface markers of CD44, CD51, CD90, and CD105 were positive on MSCs, which was consistent with our previous reports [ 7 , 8 , 10 ]. MSCs were used for further experiments, when the CD45 negative Ter119 negative cells were up to 90%.…”

“…MSCs were obtained and characterized as our previous strategy [ 7 , 8 , 10 ]. In brief, tibias and femurs of C57BL/6 mice were obtained, and muscles and connective tissues were carefully removed from the bones with surgical scissors and razor blades.…”

The Sca1+ mesenchymal stromal subpopulation promotesdendritic cell commitment in the niche

“…+ MSCs A bone-related MSC mixture and Sca1 + MSCs were isolated using a previously described method (Wen et al, 2015;Chen et al, 2016aChen et al, , 2016b. Tibias and femurs were excised from mice of 8-12 weeks old.…”

“…At P < 0.05, the difference was considered statistically significant. (Wen et al, 2015;Chen et al, 2016aChen et al, , 2016b + cells using the transwell system ( Figures 1D-1F). Taken together, these data suggest that Sca1 + MSCs promoted DC commitment and that cell-to-cell contact was needed for the promotion.…”

“…Sca1 is one of the MSC markers (Morikawa et al, 2009). Many markers were used to identify in situ and/ or to isolate MSCs in mice, such as CD29, CD51, CD73, CD90, CD105, CD106, CD135, C-KIT, nestin, PDGFRα, and Sca1, but the lack of specific markers impedes identification of MSC functions (Galderisi and Giordano, 2014 (Wen et al, 2015;Chen et al, 2016aChen et al, , 2016b. Undoubtedly, niche components are instrumental to HSC self-renewal and differentiation, but in terms of lineage commitment, there are limited data available.…”