Saturated fatty acid determination method using paired ion electrospray ionization mass spectrometry coupled with capillary electrophoresis

Lee, Ji‐Hyun; Kim, Sujin; Lee, Sul; Rhee, Jin Kyu; Lee, Soo Young; Na, Yun-Cheol

doi:10.1016/j.aca.2017.06.052

Cited by 32 publications

(29 citation statements)

References 41 publications

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“…Benzimidazoles were determined in meat at low μg/kg‐level after dispersive liquid‐liquid extraction . CE‐MS has also been applied for the analysis of fatty acids . Although anionic separation was performed, sensitivity could be increased especially for short and medium chain lengths by the addition of a cationic ion pairing reagent and ESI in positive ion mode.…”

Section: Analytical Applicationsmentioning

confidence: 99%

Recent advances in capillary electrophoresis‐mass spectrometry: Instrumentation, methodology and applications

et al. 2018

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Capillary electrophoresis (CE) offers fast and high‐resolution separation of charged analytes from small injection volumes. Coupled to mass spectrometry (MS), it represents a powerful analytical technique providing (exact) mass information and enables molecular characterization based on fragmentation. Although hyphenation of CE and MS is not straightforward, much emphasis has been placed on enabling efficient ionization and user‐friendly coupling. Though several interfaces are now commercially available, research on more efficient and robust interfacing with nano‐electrospray ionization (ESI), matrix‐assisted laser desorption/ionization (MALDI) and inductively coupled plasma mass spectrometry (ICP) continues with considerable results. At the same time, CE‐MS has been used in many fields, predominantly for the analysis of proteins, peptides and metabolites. This review belongs to a series of regularly published articles, summarizing 248 articles covering the time between June 2016 and May 2018. Latest developments on hyphenation of CE with MS as well as instrumental developments such as two‐dimensional separation systems with MS detection are mentioned. Furthermore, applications of various CE‐modes including capillary zone electrophoresis (CZE), nonaqueous capillary electrophoresis (NACE), capillary gel electrophoresis (CGE) and capillary isoelectric focusing (CIEF) coupled to MS in biological, pharmaceutical and environmental research are summarized.

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Section: Analytical Applicationsmentioning

confidence: 99%

Recent advances in capillary electrophoresis‐mass spectrometry: Instrumentation, methodology and applications

et al. 2018

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“…Recently, Lee et al. developed a CE‐PIESI‐MS method to analyze fatty acids (FAs) . In this study, di‐cationic IPRs were continuously introduced into the SL interface to generate positively charged adducts for anionic metabolites after electrophoretic separation.…”

Section: Methodological Developmentsmentioning

confidence: 99%

“…Extracted ion electropherograms of 21 linear FAs (C2∼C22:0, 100 µg/mL) obtained (A) in negative ESI mode by CE–MS using a BGE of 40% acetonitrile in 30 mM ammonium formate at pH 10 (B) in positive ESI mode with a pre‐column technique and adding 250 µL ion pair reagent 1 (IPR1) in the FA standard mixtures, (C) in positive ESI mode with an on‐column technique and adding 250 µL IPR1 into the BGE solution, and (D) in positive ESI mode with a post‐column technique using a SL containing 250 µL IPR1 in 50% IPA solution. Reproduced from with permission.…”

Section: Methodological Developmentsmentioning

confidence: 99%

CE–MS for anionic metabolic profiling: An overview of methodological developments

2019

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The efficient profiling of highly polar and charged metabolites in biological samples remains a huge analytical challenge in metabolomics. Over the last decade, new analytical techniques have been developed for the selective and sensitive analysis of polar ionogenic compounds in various matrices. Still, the analysis of such compounds, notably for acidic ionogenic metabolites, remains a challenging endeavor, even more when the available sample size becomes an issue for the total analytical workflow. In this paper, we give an overview of the possibilities of capillary electrophoresis‐mass spectrometry (CE–MS) for anionic metabolic profiling by focusing on main methodological developments. Attention is paid to the development of improved separation conditions and new interfacing designs in CE–MS for anionic metabolic profiling. A complete overview of all CE–MS‐based methods developed for this purpose is provided in table format (Table 1) which includes information on sample type, separation conditions, mass analyzer and limits of detection (LODs). Selected applications are discussed to show the utility of CE–MS for anionic metabolic profiling, especially for small‐volume biological samples. On the basis of the examination of the reported literature in this specific field, we conclude that there is still room for the design of a highly sensitive and reliable CE–MS method for anionic metabolic profiling. A rigorous validation and the availability of standard operating procedures would be highly favorable in order to make CE–MS an alternative, viable analytical technique for metabolomics.

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“…Eicosanoids are most commonly measured by GC-MS after esterification to a pentafluorobenzyl moiety [27–30], LC-MS using negative mode ionization [9,31,32], capillary electrophoresis [33,34], paired ion electrospray ionization [35,36], and LC with fluorescent detection [37]. Our method has several advances over previous measurement strategies.…”

Section: Introductionmentioning

confidence: 99%

“…Conjugation of the carboxylic acid moiety through an amide bond to the permanently positively charged group 1-(4-Aminomethyl)phenyl)pyridine-1-ium chloride (AMPP) allows for LC-MS analysis in electrospray positive ionization mode, greatly increasing sensitivity by a factor of up to 60,000 compared to underivatized fatty acids analyzed in the negative ion mode [38,39]. Paired ion electrospray ionization can also allow for detection of negative ions in positive ion mode, but its limit of detection (LOD) of 0.13 μg/mL for fatty acids is not sufficient for the detection of all AA metabolites [35]. Other groups have developed carboxylic acid derivatization and charge reversal strategies for LC-MS that have improved sensitivity by 50–1500 fold [40] and a 2500 fold improvement [41] compared to underivatized, negatively charged fatty acids.…”

Section: Introductionmentioning

confidence: 99%

A sensitive and improved throughput UPLC–MS/MS quantitation method of total cytochrome P450 mediated arachidonic acid metabolites that can separate regio-isomers and cis/trans-EETs from human plasma

Zeigler

Whittington

Sotoodehnia

et al. 2018

Chemistry and Physics of Lipids

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A method for the detection and quantification of hydroxyl and epoxy arachidonic acid (AA1) metabolites in human plasma was developed using liquid-liquid extraction, phospholipid saponification followed by derivatization of the acid moiety and liquid chromatographic tandem mass spectrometric detection. Derivatization with a pyridinium analog allowed for detection in the positive ion mode, greatly improving sensitivity and the stability of the more labile AA metabolites. The entire method utilizes a 96-well plate format, increasing sample throughput, and was optimized to measure 5-, 8-, 9-, 11-, 12-, 15-, 19-, and 20- hydroxyeicosatetraenoic acid (HETE), 5,6-, 8,9-, 11,12-, and 14,15- dihydroxyeicosatrienoic acid (DHET), and the regio- and cis-/ trans- isomers of 5,6-, 8,9-, 11,12-, and 14,15-epoxyeicosatrienoic acid (EET). The method was validated for its applicability over the FA concentration range found in human plasma. Using 100 μL aliquots of pooled human plasma, EET levels, particularly 5,6-EET, were observed to be higher than previously reported, with measured concentrations of 23.6 ng/ml for 5,6-EET, 5.6 ng/mL for 5,6-trans-EET, 8.0 ng/mL for 8,9-EET, 1.9 ng/mL for 8,9-trans-EET, 8.8 ng/mL for 11,12-EET, 3.4 ng/mL for 11,12-trans-EET, 10.7 ng/mL for 14,15-EET, and 1.7 ng/mL 14,15-trans- EET. This method is suitable for larger population studies to elucidate the complex interactions between the eicosanoids and various disease states and may be used for quantitation of a wide variety of fatty acids beyond eicosanoids from small volumes of human plasma.

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Saturated fatty acid determination method using paired ion electrospray ionization mass spectrometry coupled with capillary electrophoresis

Cited by 32 publications

References 41 publications

Recent advances in capillary electrophoresis‐mass spectrometry: Instrumentation, methodology and applications

Recent advances in capillary electrophoresis‐mass spectrometry: Instrumentation, methodology and applications

CE–MS for anionic metabolic profiling: An overview of methodological developments

A sensitive and improved throughput UPLC–MS/MS quantitation method of total cytochrome P450 mediated arachidonic acid metabolites that can separate regio-isomers and cis/trans-EETs from human plasma

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