SARS-CoV-2 Wastewater Surveillance for Public Health Action

McClary-Gutierrez, Jill S.; Mattioli, Mia; Marcenac, Perrine; Silverman, Andrea I.; Boehm, Alexandria B.; Bibby, Kyle; Balliet, Michael; Reyes, Francis L. de los; Gerrity, Daniel; Griffith, John F.; Holden, Patricia A.; Katehis, Dimitrios; Kester, Greg; LaCross, Nathan; Lipp, Erin K.; Meiman, Jonathan; Noble, Rachel T.; Brossard, Dominique; McLellan, Sandra L.

doi:10.3201/eid2709.210753

Cited by 94 publications

(66 citation statements)

References 31 publications

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“…From an epidemiological point of view, the concentration of SARS-CoV-2 in wastewater has been shown to be proportional to the number of COVID-19 cases in the catchment area, which enables wastewater-based surveillance [10][11][12][13][14]. Even though, further evaluation is required in terms of transmissibility [15] or stability [16] of SARS-CoV-2 in effluents, wastewaterbased surveillance emerged as a valuable monitoring tool [17,18] with the potential to mitigate delays of individual testing [11].…”

Section: Introductionmentioning

confidence: 99%

“…However, genotyping SARS-CoV-2 in wastewater remains challenging as the samples represent a mixture of viral genomes and, particularly with short-read data, provide insufficient phasing information to reconstruct viral haplotypes [27]. Tracing individual signature mutations in wastewater can provide valuable information on the spread of VOCs [17], and the allele frequencies of individual mutations could be linked to observations from clinical sequencing early on during the pandemic [30].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genome Sequencing of SARS-CoV-2 Allows Monitoring of Variants of Concern through Wastewater

Herold

d’Hérouël

Delogu

et al. 2021

Water

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Monitoring SARS-CoV-2 in wastewater has shown to be an effective tool for epidemiological surveillance. More specifically, RNA levels determined with RT-qPCR have been shown to track with the infection dynamics within the population. However, the surveillance of individual lineages circulating in the population based on genomic sequencing of wastewater samples is challenging, as the genetic material constitutes a mixture of different viral haplotypes. Here, we identify specific signature mutations from individual SARS-CoV-2 lineages in wastewater samples to estimate lineages circulating in Luxembourg. We compare circulating lineages and mutations to those detected in clinical samples amongst infected individuals. We show that especially for dominant lineages, the allele frequencies of signature mutations correspond to the occurrence of particular lineages in the population. In addition, we provide evidence that regional clusters can also be discerned. We focused on the time period between November 2020 and March 2021 in which several variants of concern emerged and specifically traced the lineage B.1.1.7, which became dominant in Luxembourg during that time. During the subsequent time points, we were able to reconstruct short haplotypes, highlighting the co-occurrence of several signature mutations. Our results highlight the potential of genomic surveillance in wastewater samples based on amplicon short-read data. By extension, our work provides the basis for the early detection of novel SARS-CoV-2 variants.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genome Sequencing of SARS-CoV-2 Allows Monitoring of Variants of Concern through Wastewater

Herold

d’Hérouël

Delogu

et al. 2021

Water

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show abstract

“…Clear characterization of uncertainties related to analytical methodologies would therefore facilitate interpretation of wastewater data by public health agencies. 51 Nonetheless, results from NYC's monitoring program show that relative trends in SARS-CoV-2 loads in wastewater can be evaluated and associated with trends in clinical testing data, and therefore can potentially contribute to situational awareness of disease incidence in large urban sewersheds.…”

Section: Discussionmentioning

confidence: 99%

Monitoring SARS-CoV-2 in wastewater during New York City’s second wave of COVID-19: Sewershed-level trends and relationships to publicly available clinical testing data

Hoar

Chauvin²,

Clare³

et al. 2022

Preprint

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New York City's ongoing wastewater monitoring program tracked trends in sewershed-level SARS-CoV-2 loads starting in the fall of 2020, just before the start of the City's second wave of the COVID-19 outbreak. During a five-month study period, from November 8, 2020 to April 11, 2021, viral loads in influent wastewater from each of New York City's 14 wastewater treatment plants were measured and compared to new laboratory-confirmed COVID-19 cases for the populations in each corresponding sewershed, estimated from publicly available clinical testing data. We found significant positive correlations between viral loads in wastewater and new COVID-19 cases. The strength of the correlations varied depending on the sewershed, with Spearman's rank correlation coefficients ranging between 0.38 and 0.81 (mean = 0.55). Based on a linear regression analysis of a combined data set for New York City, we found that a 1 log10 change in the SARS-CoV-2 viral load in wastewater corresponded to a 0.6 log10 change in the number of new laboratory-confirmed COVID-19 cases/day in a sewershed. An estimated minimum detectable case rate between 2 - 8 cases/day/100,000 people was associated with the method limit of detection in wastewater. This work offers a preliminary assessment of the relationship between wastewater monitoring data and clinical testing data in New York City. While routine monitoring and method optimization continue, information on the development of New York City's ongoing wastewater monitoring program may provide insights for similar wastewater-based epidemiology efforts in the future.

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“…For further information on applicability of SARS-CoV-2 sewage surveillance for supporting public health decisions and actions, see also recent reviews (Amereh et al, 2021 ; Lundy et al, 2021 ; McClary-Gutierrez et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

A State-of-the-Art Scoping Review on SARS-CoV-2 in Sewage Focusing on the Potential of Wastewater Surveillance for the Monitoring of the COVID-19 Pandemic

Ferraro

Veneri²,

Mancini

et al. 2021

Food Environ Virol

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SARS-CoV-2 Wastewater Surveillance for Public Health Action

Cited by 94 publications

References 31 publications

Genome Sequencing of SARS-CoV-2 Allows Monitoring of Variants of Concern through Wastewater

Genome Sequencing of SARS-CoV-2 Allows Monitoring of Variants of Concern through Wastewater

Monitoring SARS-CoV-2 in wastewater during New York City’s second wave of COVID-19: Sewershed-level trends and relationships to publicly available clinical testing data

A State-of-the-Art Scoping Review on SARS-CoV-2 in Sewage Focusing on the Potential of Wastewater Surveillance for the Monitoring of the COVID-19 Pandemic

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