SARS-CoV-2 electrochemical immunosensor based on the spike-ACE2 complex

Vásquez, Viviana; Navas, María Cristina; Jaimes, Javier A.; Orozco, Jahir

doi:10.1016/j.aca.2022.339718

Cited by 28 publications

(23 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The nucleocapsid and spike proteins of SARS-CoV-2 are the most promising biomarkers for antigen-based rapid diagnostics tests. A sandwich immunosensor enabled the detection of the S-protein with a good sensitivity and a LOD in the ng/mL range, using the streptavidin (poly) HRP-80 enzymatic complex as an amplification system [ 130 ]. Another approach that implemented the chronoamperometry for the fast detection (5 min) of S-protein was based on the combination of antibodies against the specific antigen for increased selectivity and DNA linkers tethered with a redox probe (ferrocene) for signal achievement [ 131 ].…”

Section: Biomarkers Involved In Inflammatory Diseasesmentioning

confidence: 99%

Point-of-care electrochemical testing of biomarkers involved in inflammatory and inflammatory-associated medical conditions

et al. 2022

View full text Add to dashboard Cite

Recent years have shown that the diagnosis and monitoring of biomarkers involved in inflammatory-associated medical conditions such as cancer, neurological disorders, viral infections, or daily physical activities offer real benefits in increasing the quality of medical care and patient life quality. In this context, the use of integrated and portable platforms as point-of-care testing devices for biomedical analysis to enable early disease diagnosis and monitoring, which can be successfully used even at the patient’s bed, is an emergency nowadays. The development of low-cost, miniaturized, and portable, user-friendly devices that provide an answer in a timely manner, such as electrochemical sensors, is relevant for the elaboration of point-of-care testing devices. This review focuses on the recent progress in bioanalysis of both specific biomarkers and inflammatory-associated biomarkers present in several diseases like neoplasia, severe neurological disorders, viral infections, and usual physical activity and provides an overview of the state of the art over the most recent electrochemical (bio)sensors for the detection of inflammation-related biomarkers. Future perspectives of point-of-care testing to improve healthcare management are also discussed. Graphical abstract

show abstract

Section: Biomarkers Involved In Inflammatory Diseasesmentioning

confidence: 99%

Point-of-care electrochemical testing of biomarkers involved in inflammatory and inflammatory-associated medical conditions

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Figure 6 b shows the scheme of a SARS-CoV-2 virus-sensing system that uses a PalmSens4 potentiostat or Senseit Smart connected to screen-printed sensors. This sensing system uses amperometry to detect angiotensin-converting enzyme host receptor (ACE2) protein by placing anti-spike antibody and anti-ACE2 antibody on the sensing electrodes [ 88 ]. The sensitivity of this device is 0.83 mA·mL/mg, and the limit of detection is 22.5 ng/mL of spike protein.…”

Section: Point-of-care Testing For Viruses Using Portable Potentiostatsmentioning

confidence: 99%

“… ( a ) SIC4341 circuit board diagram of a portable NFC potentiostat [ 87 ]. ( b ) PalmSens4 potentiostat used for SARS-CoV-2 detection [ 88 ]. ( c ) Portable potentiostat developed by Kaci et al (2022) [ 23 ].…”

Section: Figurementioning

confidence: 99%

Portable Electrochemical Biosensors Based on Microcontrollers for Detection of Viruses: A Review

et al. 2022

View full text Add to dashboard Cite

With the rise of zoonotic diseases in recent years, there is an urgent need for improved and more accessible screening and diagnostic methods to mitigate future outbreaks. The recent COVID-19 pandemic revealed an over-reliance on RT-PCR, a slow, costly and lab-based method for diagnostics. To better manage the pandemic, a high-throughput, rapid point-of-care device is needed for early detection and isolation of patients. Electrochemical biosensors offer a promising solution, as they can be used to perform on-site tests without the need for centralized labs, producing high-throughput and accurate measurements compared to rapid test kits. In this work, we detail important considerations for the use of electrochemical biosensors for the detection of respiratory viruses. Methods of enhancing signal outputs via amplification of the analyte, biorecognition of elements and modification of the transducer are also explained. The use of portable potentiostats and microfluidics chambers that create a miniature lab are also discussed in detail as an alternative to centralized laboratory settings. The state-of-the-art usage of portable potentiostats for detection of viruses is also elaborated and categorized according to detection technique: amperometry, voltammetry and electrochemical impedance spectroscopy. In terms of integration with microfluidics, RT-LAMP is identified as the preferred method for DNA amplification virus detection. RT-LAMP methods have shorter turnaround times compared to RT-PCR and do not require thermal cycling. Current applications of RT-LAMP for virus detection are also elaborated upon.

show abstract

“…Other few authors have also reported electrochemical biosensors for N gene detection [ 32 ] for the detection of spike protein [ [33] , [34] , [35] , [36] , [37] , [38] , [39] , [40] ], immunoglobulins IgG and IgM [ 15 ], and pseudotyped viral particles [ 41 , 42 ]. An immunosensor [ 43 ] and a peptide-based biosensor [ 44 ] were recently reported by our group to rapidly detect the spike protein and the viral particles in clinical samples with simple electrochemical formats and values correlated with the RT-PCR standard method.…”

Section: Introductionmentioning

confidence: 99%