Sarcostemma viminale: a potential anticancer therapy

Brestovac, Brian; Jessica, Snook; Ellison, Gaewyn; Alexander, Phillips; Tomaz, David

doi:10.1007/s00580-013-1843-0

Cited by 2 publications

(5 citation statements)

References 26 publications

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“…This also may represent transition into a dendritic cell phenotype as CD11c is highly expressed on dendritic cells. The M1-like polarisation of S. viminale-treated macrophages may contribute to its anti-cancer effects, as observed in previous studies (Reid and Betts 1977;Brestovac et al 2013).…”

Section: Discussionsupporting

confidence: 74%

“…However, a previous study reported that S. viminale induced apoptosis in the cancerous HeLa cell line (Brestovac et al 2013). Apart from cancer, studies have shown that S. viminale has been used by indigenous populations for other conditions, including stimulation of lactation, inhibition of diarrhoea, and treatment of skin and eye conditions (de Wet et al 2010).…”

Section: Discussionmentioning

confidence: 99%

“…Previous tests have shown that the S. viminale extract was highly cytotoxic at 5 %v/v (Brestovac et al 2013) and so exposure to 0.5 and 1 %v/v concentrations was chosen to test for cell viability for the RAW264.7 cells. Cell death and viability were measured using a modified Annexin V/ propidium iodide (PI) assay (Rieger et al 2011).…”

Section: Cell Viabilitymentioning

confidence: 99%

“…Whilst previous high-throughput screenings of S. viminale did not demonstrate that extracts of S. viminale could induce cytotoxicity of cancer cells (Abbott et al 1967), more recent studies have reported anti-cancer activity (Brestovac et al 2013). Other studies identified the presence of two phytochemicals, alkaloids and saponins, with known anti-cancer properties (Fabricant and Farnsworth 2001;Evans et al 2010).…”

Section: Introductionmentioning

confidence: 99%

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Sarcostemma viminale activates macrophages to a pro-inflammatory phenotype

et al. 2014

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Sarcostemma viminale (L.) R.Br, also known as caustic or milk bush, is a semi-succulent plant commonly found in the North West of Australia. Local Aboriginal populations have long used the milky white sap from this plant to treat skin cancers. An ethanol extract from S. viminale was tested by exposing the RAW264.7 cell line as an in vitro murine macrophage model, to the extract. Flow cytometric analysis was performed to determine if S. viminale skewed macrophages towards a pro-inflammatory or antiinflammatory phenotype using a number of cell surface markers. Cell culture supernatants were also analysed by cytometric bead array to determine if S. viminale exposed macrophages produced pro-inflammatory or antiinflammatory cytokines. After exposure to S. viminale, a significantly greater number of macrophages expressed proinflammatory major histocompatibility complex (MHC) class II molecules and significantly greater expression levels of the dendritic cell marker CD11c. Cytometric bead array analysis found that S. viminale induced significant amounts of the potent pro-inflammatory cytokine tumour necrosis factor (TNF) from macrophages. The markers CD40 and ICAM-1 were expressed but were not significantly different from the controls. Also, significantly higher expression of CX3CR1 indicated that macrophages were preparing to migrate. No anti-inflammatory cytokines were produced. No significant production of NO 2 − , IL-6, IFN-γ or IL-12 was found. These results demonstrate that S. viminale drives resting macrophages into a pro-inflammatory phenotype, reminiscent of activated immature dendritic cells. If this activation could be achieved in the peri-tumour environment, then S. viminale could be useful as an adjunct therapy for skin cancer.

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Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Section: Cell Viabilitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sarcostemma viminale activates macrophages to a pro-inflammatory phenotype

et al. 2014

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“…Other TM uses were not studied. Sarcostemma viminale showed potential anticancer activity supporting the TM uses in North West Australia (Brian et al, 2015). However, the use of the plant in Mozambique for treatment of Tuberculoses (Jansen and Mendes, 1983) was not verified experimentally (Luo et al, 2011).…”

Section: Resultsmentioning

confidence: 91%

A survey on traditional plants used in Al Khobah village

Abdel‐Kader

Hazazi

Elmakki

et al. 2018

Saudi Pharmaceutical Journal

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The use of traditional medicine for treatment of various diseases is a common practise in most of the developing countries including the Kingdom of Saudi Arabia especially in rural areas. In this survey, Al Khobah village was selected to study the status of Traditional Medicine. Al Khobah village is located on the Southern boarders of the kingdom and characterized by diverse topography and moderate weather. The plants with medicinal uses were collected, identified and their methods of preparation and uses were recorded. After gathering all these information from the local people, literature survey was conducted on each plant to get information about pharmacological activities and weather they support the traditional use or not. The survey revealed that the uses of some plants are consistent with the experimental data in the literature. Some other plants were studied; however, they still need pharmacological investigation to prove the claimed uses. Other plant did not subject to any scientific investigation.

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Sarcostemma viminale: a potential anticancer therapy

Cited by 2 publications

References 26 publications

Sarcostemma viminale activates macrophages to a pro-inflammatory phenotype

Sarcostemma viminale activates macrophages to a pro-inflammatory phenotype

A survey on traditional plants used in Al Khobah village

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