Sarcopenia: a histological and immunohistochemical study on age-related muscle impairment

Tarantino, Umberto; Scimeca, Manuel; Piccirilli, Eleonora; Tancredi, Virginia; Baldi, Jacopo; Gasbarra, Elena; Bonanno, Elena

doi:10.1007/s40520-015-0427-z

Cited by 29 publications

(28 citation statements)

References 32 publications

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“…Blot was stained through anti-β-actin Ab, and the content of protein was normalized with respect to β-actin and density. [41][42][43] Sections with a thickness of 5 μm were obtained from the small intestine parts and mounted on poly-l-lysine-coated slides. The parts were immersed in 0.3% H 2 O 2 for 20 min and washed with PBS.…”

Section: Western Blot Analysismentioning

confidence: 99%

Simvastatin nanoparticles attenuated intestinal ischemia/reperfusion injury by downregulating BMP4/COX-2 pathway in rats

Tong

Dong

Chai

et al. 2017

IJN

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Section: Western Blot Analysismentioning

confidence: 99%

Simvastatin nanoparticles attenuated intestinal ischemia/reperfusion injury by downregulating BMP4/COX-2 pathway in rats

Tong

Dong

Chai

et al. 2017

IJN

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“…In this scenario, aging might represents an important risk factor for CRPS. It is not surprisingly that two common agerelated musculoskeletal diseases, such as sarcopenia and osteoporosis, could impair bone healing after a fracture occurs (30). We demonstrated that the skeletal muscle of sarcopenic individuals showed substantial biological changes, including increased oxidative stress, cellular vacuolization, and mitochondrial alterations, which compromise the oxidant-scavenging systems (29).…”

Section: Epidemiology and Physiopathology Of Crps In Major Orthopaedimentioning

confidence: 81%

Algodystrophy in major orthopaedic surgery

Tarantino¹

2015

ccmbm

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SummaryComplex Regional Pain Syndrome (CRPS) describes a diversity of painful conditions following trauma, associated with abnormal regulation of blood flow and sweating, trophic changes, and edema of skin. Epidemiology of this disease is not convincing because of the difficulties and inaccuracies in the diagnosis. Several mechanisms are involved in the genesis of CRPS. The higher incidence of CRPS in women over 65 suggests that some changes involving natural and pathologic processes of aging predispose to onset a CRPS. Many features of the orthopaedic management (surgical time, immobilization, surgical incision, fracture osteosynthesis or prosthetic implants) might influence inflammation status in different way. It is mandatory to improve the understanding of both the pathogenesis of CRPS and the conditions that play a decisive role in its genesis. Furthermore it is important to find some biomarkers that allow early diagnosis before the onset of typical clinical signs.

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“…Afterwards, sections were incubated with the primary antibodies diluted 1:100 for 60 min at room temperature: anti-RUNX2 mouse monoclonal antibody (clone 1D8, Novus Biologicals, Centennial, CO 80112, USA), anti-RANKL mouse monoclonal antibody (clone 12A668, AbCam, Cambridge, UK) anti-Ki67 rabbit monoclonal antibody (clone 30-9, Ventana, Tucson, USA), anti-ER rabbit monoclonal antibody (clone SP1, Novus Biologicals, Centennial, CO 80112, USA), anti-BMP-2 mouse monoclonal antibody (clone 9E10G12; Novus Biologicals, Centennial, CO 80112, USA), and anti-PTX3 rat monoclonal antibody (clone MNB1; Abcam, UK). Reactions were detected by using HRP-DAB Detection Kit (UCS Diagnostic, Italy) [17]. RUNX2 (streptavidin-texas-red) and RANKL (streptavidin-FITC) were detected simultaneously by immunofluorescence.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Breast-Specific Gamma Imaging with [99mTc]Tc-Sestamibi: An In Vivo Analysis for Early Identification of Breast Cancer Lesions Expressing Bone Biomarkers

Urbano

Scimeca

Russo

et al. 2020

JCM

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The main purpose of this pilot investigation was to evaluate the possible relationship among [99mTc]Tc-Sestamibi uptake, the presence of breast osteoblast-like cells, and the expression of molecules involved in bone metabolism, such as estrogen receptor, bone morphogenetic proteins-2, and PTX3. To this end, forty consecutive breast cancer patients who underwent both breast-specific gamma imaging with [99mTc]Tc-Sestamibi and breast bioptic procedure were retrospectively enrolled. From each diagnostic paraffin block collected in the study, histological diagnosis, immunohistochemical investigations, and energy dispersive X-ray microanalysis were performed. Our data highlight the possible use of breast-specific gamma imaging with [99mTc]Tc-Sestamibi for the early detection of breast cancer lesions expressing bone biomarkers in the presence of breast osteoblast-like cells. Specifically, we show a linear association among sestamibi uptake, the presence of breast osteoblast-like cells, and the expression of estrogen receptor, bone morphogenetics proteins-2, and PTX3. Notably, we also observed an increase of [99mTc]Tc-Sestamibi in breast cancer lesions with magnesium-substituted hydroxyapatite. In conclusion, in this pilot study we evaluated data from the nuclear medicine unit and anatomic pathology department on breast cancer osteotropism, identifying a new possible interpretation of Breast Specific Gamma Imaging with [99mTc]Tc-Sestamibi analysis.

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Sarcopenia: a histological and immunohistochemical study on age-related muscle impairment

Abstract: The characterization of molecular mechanisms underlying the bone-muscle crosstalk could open new therapeutic perspectives in elderly diseases.

Cited by 29 publications

References 32 publications

Simvastatin nanoparticles attenuated intestinal ischemia/reperfusion injury by downregulating BMP4/COX-2 pathway in rats

Simvastatin nanoparticles attenuated intestinal ischemia/reperfusion injury by downregulating BMP4/COX-2 pathway in rats

Algodystrophy in major orthopaedic surgery

Breast-Specific Gamma Imaging with [99mTc]Tc-Sestamibi: An In Vivo Analysis for Early Identification of Breast Cancer Lesions Expressing Bone Biomarkers

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