Sarconesiopsis magellanica (Diptera: Calliphoridae) excretions and secretions have potent antibacterial activity

Díaz-Roa, Andrea; Gaona, María Antonia; Segura, Nidya Alexandra; Suárez, Diana; Patarroyo, Manuel A.; Bello, Felio J.

doi:10.1016/j.actatropica.2014.04.018

Cited by 20 publications

(17 citation statements)

References 57 publications

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“…There were no marked differences regarding the evaluation of wounds treated with haemolymph from both blowfly species, probably due to a similar effect regarding its components' activity. However, wound size became considerably reduced on day 23, mainly for those treated with S. magellanica haemolymph, differently to wounds treated with the same substance but derived from L. sericata , suggesting that, in spite of the antibacterial properties demonstrated by the haemolymph, the effect was more efficient on those from the first species, similar to the results obtained in a previous in vitro study [ 11 ]; this evaluated antibacterial activity of larval ES from both species, demonstrating that S. magellanica -derived substances were more potent and effective than those taken from L. sericata . However, wounds treated with fat body extracts had a greater effect compared to when using haemolymph; for example, a more reduced wound area with abundant granulation tissue around the edges and moderate exudate was observed on day 12 ( Figure 2 ), compared to the effect of treatment involving haemolymph, leading to more favourable evolution by day 23, as there was almost total wound contraction for ulcers treated with fat body from both blowfly species.…”

Section: Discussionsupporting

confidence: 86%

“…Likewise, this species' proteolytic enzymes have been identified and characterised from excretions and secretions (ES) taken from first, second, and third instar larvae [ 3 ]. It has also been shown that this blowfly's larval ES have potent antibacterial activity [ 11 ], thereby suggesting the presence of molecules in other larval tissues, having similar functions, which could be of interest in treating chronic and infected ulcers.…”

Section: Introductionmentioning

confidence: 99%

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Evaluating the Effect ofSarconesiopsis magellanica(Diptera: Calliphoridae) Larvae-Derived Haemolymph and Fat Body Extracts on Chronic Wounds in Diabetic Rabbits

Góngora

Díaz-Roa

Ramírez-Hernández

et al. 2015

Journal of Diabetes Research

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We evaluated extracts taken from S. magellanica third instar larvae fat body and haemolymph using a diabetic rabbit model and compared this to the effect obtained with the same substances taken from Lucilia sericata larvae. Alloxan (a toxic glucose analogue) was used to induce experimental diabetes in twelve rabbits. Dorsal wounds were made in each animal and they were infected with Staphylococcus aureus and Pseudomonas aeruginosa. They were then treated with haemolymph and lyophilized extracts taken from the selected blowflies' larvae fat bodies. Each wound was then evaluated by using rating scales and histological analysis. More favourable scores were recorded on the PUSH and WBS scales for the wounds treated with fat body derived from the larvae of both species compared to that obtained with haemolymph; however, wounds treated with the substances taken from S. magellanica had better evolution. Histological analysis revealed that treatment led to tissue proliferation and more effective neovascularisation in less time with both species' fat body extracts compared to treatment with just haemolymph. The results suggest the effectiveness of the substances evaluated and validate them in the animal model being used here as topical agents in treating chronic wounds.

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Section: Discussionsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Evaluating the Effect ofSarconesiopsis magellanica(Diptera: Calliphoridae) Larvae-Derived Haemolymph and Fat Body Extracts on Chronic Wounds in Diabetic Rabbits

Góngora

Díaz-Roa

Ramírez-Hernández

et al. 2015

Journal of Diabetes Research

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“…Insect capture and colony maintenance followed a previously described procedure ( Diaz-Roa et al, 2014 ). Adult S. magellanica forms were captured in the mountainous part of Bogotá’s Parque Nacional; the park is located 2,600 masl (4°37′8.90N; 74°3′27.73W).…”

Section: Methodsmentioning

confidence: 99%

“…S. magellanica was reported as the first species colonizing decomposing pigs in Bogotá (an animal bio-model similar to that of humans) ( Goff, 2001 ). Its antibacterial activity has been confirmed, giving better results than those for L. sericata ( Diaz-Roa et al, 2014 ) and it has already been evaluated regarding LT, leading to good effects concerning diabetic wound cicatrisation ( Cruz-Saavedra et al, 2016 ) and in Leishmania lesions ( Cruz-Saavedra et al, 2016 ).…”

Section: Introductionmentioning

confidence: 97%

Sarconesin: Sarconesiopsis magellanica Blowfly Larval Excretions and Secretions With Antibacterial Properties

et al. 2018

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Larval therapy (LT) is an alternative treatment for healing chronic wounds; its action is based on debridement, the removal of bacteria, and stimulating granulation tissue. The most important mechanism when using LT for combating infection depends on larval excretions and secretions (ES). Larvae are protected against infection by a spectrum of antimicrobial peptides (AMPs); special interest in AMPs has also risen regarding understanding their role in wound healing since they degrade necrotic tissue and kill different bacteria during LT. Sarconesiopsis magellanica (Diptera: Calliphoridae) is a promising medically-important necrophagous fly. This article reports a small AMP being isolated from S. magellanica ES products for the first time; these products were obtained from third-instar larvae taken from a previously-established colony. ES were fractionated by RP-HPLC using C18 columns for the first analysis; the products were then lyophilised and their antimicrobial activity was characterized by incubation with different bacterial strains. These fractions’ primary sequences were determined by mass spectrometry and de novo sequencing; five AMPs were obtained, the Sarconesin fraction was characterized and antibacterial activity was tested in different concentrations with minimum inhibitory concentrations starting at 1.2 μM. Potent inhibitory activity was shown against Gram-negative (Escherichia coli D31, E. coli DH5α, Salmonella enterica ATCC 13314, Pseudomonas aeruginosa 27853) and Gram-positive (Staphylococcus aureus ATCC 29213, S. epidermidis ATCC 12228, Micrococcus luteus A270) bacteria. Sarconesin has a significant similarity with Rho-family GTPases which are important in organelle development, cytoskeletal dynamics, cell movement, and wound repair. The data reported here indicated that Sarconesin could be an alternative candidate for use in therapeutics against Gram-negative and Gram-positive bacterial infections. Our study describes one peptide responsible for antibacterial activity when LT is being used. The results shown here support carrying out further experiments aimed at validating S. magellanica AMPs as novel resources for combating antibacterial resistance.

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“…Blowfly excreta and secretion extracted from Sarconesiopsis magellanica and Lucilia sericata (Calliphoridae) larvae were evaluated for antibacterial activity against a panel of six bacteria split evenly between Gram positive and Gram negative bacterium 148 . Both species were determined effective against the selected strains however the S. magellanica extract required a lower concentration to ensure therapeutic efficacy.…”

Section: Diptera (Flies and Mosquitoes)mentioning

confidence: 99%

Insects: an underrepresented resource for the discovery of biologically active natural products

Seabrooks¹,

Hu²

2017

Acta Pharmaceutica Sinica B

105

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Nature has been the source of life-changing and -saving medications for centuries. Aspirin, penicillin and morphine are prime examples of Nature׳s gifts to medicine. These discoveries catalyzed the field of natural product drug discovery which has mostly focused on plants. However, insects have more than twice the number of species and entomotherapy has been in practice for as long as and often in conjunction with medicinal plants and is an important alternative to modern medicine in many parts of the world. Herein, an overview of current traditional medicinal applications of insects and characterization of isolated biologically active molecules starting from approximately 2010 is presented. Insect natural products reviewed were isolated from ants, bees, wasps, beetles, cockroaches, termites, flies, true bugs, moths and more. Biological activities of these natural products from insects include antimicrobial, antifungal, antiviral, anticancer, antioxidant, anti-inflammatory and immunomodulatory effects.

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Sarconesiopsis magellanica (Diptera: Calliphoridae) excretions and secretions have potent antibacterial activity

Cited by 20 publications

References 57 publications

Evaluating the Effect ofSarconesiopsis magellanica(Diptera: Calliphoridae) Larvae-Derived Haemolymph and Fat Body Extracts on Chronic Wounds in Diabetic Rabbits

Evaluating the Effect ofSarconesiopsis magellanica(Diptera: Calliphoridae) Larvae-Derived Haemolymph and Fat Body Extracts on Chronic Wounds in Diabetic Rabbits

Sarconesin: Sarconesiopsis magellanica Blowfly Larval Excretions and Secretions With Antibacterial Properties

Insects: an underrepresented resource for the discovery of biologically active natural products

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