Sarconesin: Sarconesiopsis magellanica Blowfly Larval Excretions and Secretions With Antibacterial Properties

Díaz-Roa, Andrea; Patarroyo, Manuel A.; Bello, Felio J.; Silva, Pedro I. da

doi:10.3389/fmicb.2018.02249

Cited by 20 publications

(25 citation statements)

References 86 publications

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“…In recent research, a cecropin-like peptide from maggot had antimicrobial activity against Gram-negative bacteria and immunomodulatory activities [ 26 ]. We speculated that one peptide responsible for antibacterial activity [ 34 ] had anti-inflammatory effects on IBD. Our present study demonstrated that the maggot protein could suppress DSS-induced colitis in mice and improve acute colonic injuries.…”

Section: Discussionmentioning

confidence: 99%

Maggot protein ameliorates dextran sulphate sodium-induced ulcerative colitis in mice

Wang

Luo

et al. 2018

Bioscience Reports

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Ulcerative colitis (UC) is a common chronic remitting disease but without satisfactory treatment. Maggots are known as a traditional Chinese medicine named as ‘wu gu chong’. The aim of the present study was to investigate the therapeutic effect of the maggot protein on dextran sulphate sodium (DSS)-induced colitis in C57BL/6 mice. In the present study, female C57BL/6 mice were given sterile water containing 3% DSS to establish the model of UC. Mice were randomly divided into five groups: control group (sterile water), model group (DSS), treatment group (DSS + maggot protein), mesalazine group (DSS + mesalazine), and maggot protein group (sterile water + maggot protein). The mental state, defecate traits, and changes in body weights were recorded daily. The disease activity index (DAI) as a disease severity criterion was calculated based on body weights and stool consistency and bleeding. All the mice were killed on the 12th day. Colon length, colon histological changes, and other inflammatory factors were analyzed and evaluated. The results showed that colitis models of mice were established successfully. Administration of maggot protein markedly suppressed the severity of UC compared with the DSS model group. Furthermore, maggot protein potently ameliorated DSS-induced weight loss, colon shortening, and colon histological injury. Moreover, the maggot protein exerted anti-inflammatory effects via inhibition of the activation of the nuclear factor κB (NFκB) signaling pathway. In summary, treatment by maggot protein was able to improve not only the symptoms of colitis, but also the microscopic inflammation in mice with DSS-induced colitis. The present study may have implications for developing an effective therapeutic strategy for inflammatory bowel diseases (IBDs).

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Section: Discussionmentioning

confidence: 99%

Maggot protein ameliorates dextran sulphate sodium-induced ulcerative colitis in mice

Wang

Luo

et al. 2018

Bioscience Reports

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“…For example, larval therapy is used widely to cure non-healing wounds. This involves, the application of mature blow fly larvae belonging to Sarconesiopsis genus on an open wound, resulting in the secretion of antimicrobial peptides and metabolites (Diaz-Roa et al 2018). Maggot debridement therapy is effective to cure severe necrotizing fasciitis, caused by more than one type of bacteria such as MRSA, Streptococcus pyogenes , enterococci, E. coli , P. aeruginosa , Clostridium and Bacteroides species (Maya et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Biologically active metabolite(s) from haemolymph of red-headed centipede Scolopendra subspinipes possess broad spectrum antibacterial activity

et al. 2019

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The discovery of novel antimicrobials from animal species under pollution is an area untapped. Chinese red-headed centipede is one of the hardiest arthropod species commonly known for its therapeutic value in traditional Chinese medicine. Here we determined the antibacterial activity of haemolymph and tissue extracts of red-headed centipede, Scolopendra subspinipes against a panel of Gram-positive and Gram-negative bacteria. Lysates exhibited potent antibacterial activities against a broad range of bacteria tested. Chemical characterization of biologically active molecules was determined via liquid chromatography mass spectrometric analysis. From crude haemolymph extract, 12 compounds were identified including: (1) l -Homotyrosine, (2) 8-Acetoxy-4-acoren-3-one, (3) N -Undecylbenzenesulfonic acid, (4) 2-Dodecylbenzenesulfonic acid, (5) 3 H -1,2-Dithiole-3-thione, (6) Acetylenedicarboxylate, (7) Albuterol, (8) Tetradecylamine, (9) Curcumenol, (10) 3-Butylidene-7-hydroxyphthalide, (11) Oleoyl Ethanolamide and (12) Docosanedioic acid. Antimicrobial activities of the identified compounds were reported against Gram-positive and Gram-negative bacteria, fungi, viruses and parasites, that possibly explain centipede’s survival in harsh and polluted environments. Further research in characterization, molecular mechanism of action and in vivo testing of active molecules is needed for the development of novel antibacterials.

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“…Antimicrobial peptides and proteins show a wide distribution and have been isolated from nearly all living organisms. [8][9][10][11][12][13][14][15][16][17][18][19][20][21] Beyond their antimicrobial effects, several AMPs have been shown to have other biological activities. 11,[22][23][24][25] One such protein group that represents the duplicity of activities for the same molecules is the tachykinin family.…”

Section: Introductionmentioning

confidence: 99%

“…Antimicrobial peptides and proteins show a wide distribution and have been isolated from nearly all living organisms. 8 - 21 …”

Section: Introductionmentioning

confidence: 99%

Two Tachykinin-Related Peptides with Antimicrobial Activity Isolated from Triatoma infestans Hemolymph

et al. 2020

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Antimicrobial peptides and proteins (AMPs) are molecules that can interact with microbial cells and lead to membrane disruption or intracellular molecule interactions and death. Several molecules with antimicrobial effects also present other biological activities. One such protein group representing the duplicity of activities is the tachykinin family. Tachykinins (TKs) form a family of neuropeptides in vertebrates with a consensus C-terminal region (F-X-G-Y-R-NH2). Invertebrate TKs and TK-related peptides (TKRPs) are subfamilies found in invertebrates that present high homology with TKs and have similar biological effects. Several of these molecules have already been described but reports of TKRP in Hemiptera species are limited. By analyzing the Triatoma infestans hemolymph by reversed-phase high-performance liquid chromatography, biological assays, and mass spectrometry, two antimicrobial molecules were isolated and identified as TKRPs, which we named as TRP1-TINF and TRP2-TINF (tachykinin-related peptides I and II from T. infestans). TRP1-TINF is a random secondary structure peptide with 9 amino acid residues. It is susceptible to aminopeptidases degradation and is active mainly against Micrococcus luteus (32 μM). TRP2-TINF is a 10-amino acid peptide with a 310 helix secondary structure and is susceptible to carboxypeptidases degradation. It has major antimicrobial activity against both Pseudomonas aeruginosa and Escherichia coli (45 μM). Neither molecule is toxic to human erythrocytes and both present minor toxicity toward Vero cells at a concentration of 1000 μM. As the first description of TKRPs with antimicrobial activity in T. infestans, this work contributes to the wider comprehension of the insects’ physiology and describes pharmacological relevant molecules.

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Sarconesin: Sarconesiopsis magellanica Blowfly Larval Excretions and Secretions With Antibacterial Properties

Cited by 20 publications

References 86 publications

Maggot protein ameliorates dextran sulphate sodium-induced ulcerative colitis in mice

Maggot protein ameliorates dextran sulphate sodium-induced ulcerative colitis in mice

Biologically active metabolite(s) from haemolymph of red-headed centipede Scolopendra subspinipes possess broad spectrum antibacterial activity

Two Tachykinin-Related Peptides with Antimicrobial Activity Isolated from Triatoma infestans Hemolymph

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