Sarcocystis

Franco, Cecilia Decker; Schnittger, Leonhard; Florín-Christensen, Mónica

doi:10.1007/978-3-319-70132-5_4

Cited by 13 publications

(23 citation statements)

References 58 publications

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“…Due to the wide variety of Sarcocystis species, and the wide variety of definitive and intermediate hosts (FRANCO et al, 2018;, it is difficult to identify the source of goose contamination. Waterfowl that inhabit and feed in shallow waters are more commonly infected than those that inhabit deeper waters (CHEN et al, 2015), suggesting that the infection occurs most commonly in shallow and marshy waters.…”

Section: Discussionmentioning

confidence: 99%

“…is an obligate intracellular coccidian parasite that requires an intermediate and a definitive host (usually an herbivore and carnivore, respectively) and can affect several species of mammals and birds (El-MORSEY et al, 2015). The genus is composed of more than 100 species (some of which are zoonotic) that differ in their levels of pathogenicity, host specificity, cyst structure and location (FRANCO et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Serosurvey of Toxoplasma gondii, Sarcocystis sp. and Neospora caninum in geese (Anser sp.) from urban parks and captivity

Konell

Sato

Stival³

et al. 2019

Rev. Bras. Parasitol. Vet.

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Geese, ducks, mallards, and swans are birds of the order Anseriformes, which are found in the wild, in zoos and parks, and raised for meat consumption. Toxoplasma gondii, Sarcocystis sp., and Neospora caninum are protozoans of several species of animals. Wild and domestic birds can serve as intermediate hosts, disseminators and potential sources of infection of these protozoa to humans through contaminated meat. The aims of this study were: (i) to perform a serological survey of T. gondii, Sarcocystis sp. and N. caninum in geese (Anser sp.) from public parks and from captivity and (ii) to compare seroprevalence between these two locations. Antibodies were detected by Immunofluorescence antibody test using the serum of 149 geese. Antibodies to Sarcocystis sp., T. gondii, and N. caninum were detected in 28.18%, 18% and 0.67% of geese, respectively; 57% of geese from urban parks and 26.53% of geese from captivity were seropositive for at least one protozoa. The results indicate environmental contamination, particularly for the occurrence of antibodies against T. gondii – a zoonosis that causes toxoplasmosis and is transmitted through oocyte ingestion. This is the first serological survey of T. gondii, Sarcocystis sp. and N. caninum in geese from urban parks in Curitiba, Brazil.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Serosurvey of Toxoplasma gondii, Sarcocystis sp. and Neospora caninum in geese (Anser sp.) from urban parks and captivity

Konell

Sato

Stival³

et al. 2019

Rev. Bras. Parasitol. Vet.

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“…However, it may lead to signifi cant economic losses in the form of abortion, reduced milk yield, neurologic signs, and loss of weight. Moreover, down-regulation of the meat quality, decreased wool and fi ber production adding to its zoonotic importance [2,3]. Sarcocystosis has a worldwide distribution with a high prevalence rate both in rural and developed areas [4].…”

Section: Introductionmentioning

confidence: 99%

Detection of Humoral and Cellular Immune Responses in Buffaloes Naturally Infected with Sarcocystosis with Risk Factor Assessment

2019

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Sarcocystosis is a silent, parasitic disease which affects various animal species and causes significant economic losses. It is caused by a number of different intracellular Sarcocystis spp. This study was aimed to detect the host humoral and cellular immune response due to natural infection. Adding to the determination of the infection rate in Monufia Governorate, Egypt. A total number of 127 Egyptian buffaloes (Bubalus bubalis); 30 males and 97 females between 2-11 years of age were examined during 2018. An infection rate of 74% (94/127) was detected by macroscopic examination. The old age females were found to be at a high risk of 90.7% (88/97) in comparison with the young males 20% (6/30). Immunologically, the cellular and humoral immune response was determined using ELISA. A marked down-regulation of the proinflammatory Th-1 cytokine (IFN-γ) and up-regulation of the anti-inflammatory Th-2 cytokine (IL-5) adding to a high level of IgG and IgE were detected in the infected animals compared to the non infected ones. The local cellular immune response in the infected tissues was characterized by an accumulation of mixed inflammatory cells, granuloma formation, eosinophilic infiltration, muscular edema, and necrotic degeneration. In conclusion, the Sarcocystis infection rate in the naturally infected buffaloes in Monufia Governorate was high. This is the first study to provide a fundamental insight into the immune profile in buffaloes infected with Sarcocystis spp. So, it will provide valuable insights to develop novel effective vaccines in future studies. Moreover, sensitive and specific tools should be established for the accurate diagnosis of this disease in the different Egyptian governorates through well-structured serological surveys.

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“…One of the limitations for the production and commercialization of SAC meat is the frequent finding of macroscopic cysts (1–5 mm) resembling rice grains in skeletal muscles (Decker Franco et al., 2018; Moré et al., 2016; Saeed et al., 2018). Cysts essentially consist of encapsulated conglomerates of up to 2 × 10 7 protozoan parasites of the Sarcocystis aucheniae species, and have a brain-like structure when examined under electron microscopy (Carletti et al., 2013; Decker Franco et al., 2018; Moré et al., 2016). Cysts are usually very abundant and give an unpleasant aspect to SAC meat, which leads to confiscation of carcasses by sanitary authorities or devaluation of their commercial value.…”

Section: Introductionmentioning

confidence: 99%

“…Most aspects of S. aucheniae cycle are yet to be elucidated , but can be inferred from studies carried out in other Sarcocystis species (Dubey et al., 2015; Decker Franco et al., 2018; Saeed et al., 2018) . These coccidians complete their life cycle in an intermediate herbivore and a carnivore definitive host, in which, respectively, the asexual and sexual reproduction phases take place.…”

Section: Introductionmentioning

confidence: 99%

Detection of Sarcocystis aucheniae in blood of llama using a duplex semi-nested PCR assay and its association with cyst infestation

Franco

Romero

Ferrari

et al. 2018

Heliyon

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The protozoon Sarcocystis aucheniae is the causative agent of South American camelid (SAC) sarcocystosis. Infections are characterized by the presence of cysts in muscles which are in size and appearance similar to rice grains. As consumption of insufficiently cooked infected meat produces gastroenteritis, cyst-containing SAC meat is confiscated by sanitary authorities or depreciated with serious economic consequences for SAC breeders. In this work, a duplex semi-nested PCR was designed to simultaneously detect parasite and llama DNA in host blood samples. Species-specific regions of S. aucheniae 18S rRNA gene and Lama glama 16S mitochondrial gene were amplified, yielding bands of 583 and 257 bp, respectively, and separated by gel electrophoresis. The method proved to be highly sensitive, with a detection limit lower than one parasite per milliliter blood, and the inclusion of primers to detect llama-specific DNA resulted useful as a methodological control. Blood samples collected from llamas of Argentina and Bolivia (n = 225) were analyzed using this method, and 18.7 % resulted positive for S. aucheniae. No correlation was found between PCR results and llama age, sex or the finding of macroscopic cysts in meat after slaughter. Lack of molecular detection in the blood of some llamas harboring macrocysts suggests that parasite circulation in the bloodstream after encystment is under the detection threshold of the test or even absent, while PCR positive results in cyst-infested animals suggests that prior exposure to the parasite does not impede subsequent infections. The described method can be useful to detect active foci of infection, to assess the effectiveness of parasiticide treatments, and for the surveillance and tracing of definitive hosts.

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Sarcocystis

Cited by 13 publications

References 58 publications

Serosurvey of Toxoplasma gondii, Sarcocystis sp. and Neospora caninum in geese (Anser sp.) from urban parks and captivity

Serosurvey of Toxoplasma gondii, Sarcocystis sp. and Neospora caninum in geese (Anser sp.) from urban parks and captivity

Detection of Humoral and Cellular Immune Responses in Buffaloes Naturally Infected with Sarcocystosis with Risk Factor Assessment

Detection of Sarcocystis aucheniae in blood of llama using a duplex semi-nested PCR assay and its association with cyst infestation

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