Sand Culture of Cotton Plants

Olcott, Henry Steel

doi:10.1126/science.89.2322.608

Cited by 3 publications

(2 citation statements)

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“…The test cottons G. raimondii , G. arboreum L. Shixiya 1, and G. hirsutum L. TM-1were provided from the Institute of Cotton Research, Chinese Academy of Agricultural Sciences (CAAS, Anyang, China). Cotton plants were cultivated using the sand culture method [ 36 ]. Three seedlings at trefoil stage were exposed to low temperature (4.0 °C, 24 h), salt (150 mM NaCl, 24 h) and drought (sand moisture content 5.0 %), respectively.…”

Section: Methodsmentioning

confidence: 99%

Genome-wide Identification and analysis of the stress-resistance function of the TPS (Trehalose-6-Phosphate Synthase) gene family in cotton

Wang

et al. 2016

BMC Genet

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BackgroundTrehalose (a-D-glucopyranosyl a-D-glucopyranoside) is a nonreducing disaccharide and is widely distributed in bacteria, fungi, algae, plants and invertebrates. In the study, the identification of trehalose-6-phosphate synthase (TPS) genes stress-related in cotton, and the genetic structure analysis and molecular evolution analysis of TPSs were conducted with bioinformatics methods, which could lay a foundation for further research of TPS functions in cotton.ResultsThe genome information of Gossypium raimondii (group D), G. arboreum L. (group A), and G. hirsutum L. (group AD) was used in the study. Fifty-three TPSs were identified comprising 15 genes in group D, 14 in group A, and 24 in group AD. Bioinformatics methods were used to analyze the genetic structure and molecular evolution of TPSs. Real-time PCR analysis was performed to investigate the expression patterns of gene family members. All TPS family members in cotton can be divided into two subfamilies: Class I and Class II. The similarity of the TPS sequence is high within the same species and close within their family relatives. The genetic structures of two TPS subfamily members are different, with more introns and a more complicated gene structure in Class I. There is a TPS domain(Glyco transf_20) at the N-terminal in all TPS family members and a TPP domain(Trehalose_PPase) at the C-terminal in all except GrTPS6, GhTPS4, and GhTPS9. All Class II members contain a UDP-forming domain. The responses to environmental stresses showed that stresses could induce the expression of TPSs but the expression patterns vary with different stresses.ConclusionsThe distribution of TPSs varies with different species but is relatively uniform on chromosomes. Genetic structure varies with different gene members, and expression levels vary with different stresses and exhibit tissue specificity. The upregulated genes in upland cotton TM-1 is significantly more than that in G. raimondii and G. arboreum L. Shixiya 1.Electronic supplementary materialThe online version of this article (doi:10.1186/s12863-016-0360-y) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Genome-wide Identification and analysis of the stress-resistance function of the TPS (Trehalose-6-Phosphate Synthase) gene family in cotton

Wang

et al. 2016

BMC Genet

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“…All materials were provided from the Institute of Cotton Research, Chinese Academy of Agricultural Sciences (CAAS, Anyang, China). Seedlings were raised by sand culture (Olcott, 1939).…”

Section: Methodsmentioning

confidence: 99%

Genome‐wide Identification and Structural Analysis of Pyrophosphatase Gene Family in Cotton

Zhao

Yin

et al. 2016

Crop Science

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Pyrophosphatase is a hydrolytic enzyme that utilizes pyrophosphate as a substrate. H+‐PPase, an important proton pump on the vacuolar membrane, plays an important role in regulating cell expansion, H+ electrochemical gradient, and secondary active transport of inorganic ions, organic acids and saccharides. Under low temperature, drought, high NaCl concentration, and hypoxia, H+‐PPase gene expression is promoted to maintain the intracellular balance and enhance the ability of stress resistance in plants. The GhVP gene family is a class of H+‐PPase genes in Gossypium hirsutum L. The present study identified 13 H+‐PPase genes from diploid Gossypium raimondii Ulbrich (genome D) and Gossypium arboreum L. (genome A), and analyzed their genetic structures and systematic evolution. The 13 H+‐PPase genes, including six and seven from diploid G. arboreum and G. raimondii, respectively, were obtained with GhVP as a reference. The H+‐PPase genes belong to a relatively conserved family in plant evolution. Phylogenetic analysis indicated that the genes of the same species showed high sequence similarities and relatively close genetic relationships. Gene structural analysis indicated that most of the H+‐PPase genes had 7–8 exons, with a few having a variable number (1–14) of exons. By analyzing the conserved domain, the H+‐PPase genes showed several relatively conserved structural domains, such as a myristyl site, casein kinase site II, and protein kinase phosphorylation site. These findings provide a basis for the further characterization of the H+‐PPase gene function in cotton. Analysis by qRT‐PCR showed that a different expression level of gene members in the V‐PPase family existed in different tissues; for example, gene expression was highest in leaves and lowest in fibers to suggest that V‐PPase expression is tissue‐specific. Moreover, with the salt stress intensity increasing, the changes of expression level of the V‐PPase genes were different.

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Uptake, translocation and impact of green synthesized nanoceria on growth and antioxidant enzymes activity of Solanum lycopersicum L.

Singh

Hussain²,

Singh

et al. 2019

Ecotoxicology and Environmental Safety

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Sand Culture of Cotton Plants

Cited by 3 publications

References 2 publications

Genome-wide Identification and analysis of the stress-resistance function of the TPS (Trehalose-6-Phosphate Synthase) gene family in cotton

Genome-wide Identification and analysis of the stress-resistance function of the TPS (Trehalose-6-Phosphate Synthase) gene family in cotton

Genome‐wide Identification and Structural Analysis of Pyrophosphatase Gene Family in Cotton

Uptake, translocation and impact of green synthesized nanoceria on growth and antioxidant enzymes activity of Solanum lycopersicum L.

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