Sampling Blood from Birds: A Technique and an Assessment of Its Effect

Hoysak, Drew J.; Weatherhead, Patrick J.

doi:10.2307/1368207

Cited by 82 publications

(58 citation statements)

References 23 publications

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“…First, a baseline blood sample (~35-40 µL) was taken from each nestling within 3 min of approaching the nest (see Romero & Reed 2005). Blood was extracted via brachial venipuncture using a 26-gauge needle and quickly drawn into heparinized microhematocrit capillary tubes (Hoysak & Weatherhead 1991), after which, blood flow was staunched by gently applying pressure with a sterile piece of cotton.…”

Section: Blood Collection and Corticosterone Assaymentioning

confidence: 99%

Experimental effects of early‐life corticosterone on the hypothalamic–pituitary–adrenal axis and pre‐migratory behaviour in a wild songbird

et al. 2015

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Although laboratory studies have shown that chronic exposure to elevated glucocorticoids during development has profound effects on animals, we still have a poor understanding of the consequences of early-life stress on wild individuals. In an island population of Savannah sparrows (Passerculus sandwichensis), I examined multiple hypotheses to explain how elevated corticosterone exposure during the nestling period influenced both hypothalamic-pituitaryadrenal (HPA) axis function and the subsequent movement and survival of young after they fledged the nest. Corticosterone-treated nestlings had higher baseline corticosterone levels and lower stress reactivity than untreated individuals, and were more sensitive to inclement weather.Corticosterone-treated individuals also had higher rates of temporary emigration outside of the study site than sham or controls. My results provide support for both the ceiling hypothesis and CORT-activity hypothesis, and highlight the importance of tracking individuals across multiple life stages to understand how early life events carry-over to influence both physiology and behaviour.iii ACKNOWLEDGEMENTS

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Section: Blood Collection and Corticosterone Assaymentioning

confidence: 99%

Experimental effects of early‐life corticosterone on the hypothalamic–pituitary–adrenal axis and pre‐migratory behaviour in a wild songbird

et al. 2015

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“…For instance, the blood sampling process can result in nest desertion (Colwell et al 1988;Kania 1992;Criscuolo 2001) or even mortality in some species (Brown and Brown 2009). Many studies have investigated the effects of the blood sampling process on terrestrial bird performances (Wingfield and Farner 1976;Dufty 1988;Hoysak and Weatherhead 1991;Lanctot 1994;Lubjuhn et al 1998;Perkins et al 2004;Schmoll et al 2004;Sheldon et al 2008), and this has led to the development of guidelines aimed at the ethical collection of blood that minimize the potential deleterious effects on birds Gaunt et al 1997;Sheldon et al 2008). However and surprisingly, accurate estimates of the impact of blood collection on performances of seabirds have rarely been conducted (Sheldon et al 2008, but see Brown 1995Van den Brink and Pigott 1996) despite an increasing number of eco-physiological studies focusing on these species (e.g., Kitaysky et al 1999;Criscuolo et al 2002;Hall et al 2004;Chastel et al 2005;Cherel et al 2005;Angelier et al 2007bAngelier et al , 2010.…”

Section: Introductionmentioning

confidence: 99%

Capture and blood sampling do not affect foraging behaviour, breeding success and return rate of a large seabird: the black-browed albatross

2010

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During the last decades, eco-physiological studies have usually relied on the collection of blood from wild organisms in order to obtain relevant physiological measures. However, accurate estimates of the impact of capture and blood collection on performances of Polar seabird species have rarely been conducted. We investigated for the first time the effects of a blood sampling process on subsequent foraging behaviour, reproductive performance and return rate of black-browed albatrosses (Thalassarche melanophris) at Kerguelen Islands. We did not find any evidence that the blood sampling process as conducted in our study had detrimental effects on the breeding or foraging strategies or performance of blackbrowed albatrosses. Because blood collection can be performed in several different ways, we recommend that eco-physiologists conduct pilot studies to test whether their blood sampling process affects the performances of their study species.

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“…High glucocorticoid levels are often also associated with a rise in body temperature (Cabanac and Aizawa 2000;Cabanac and Guillemette 2001), It is, however, usually assumed that bleeding and handling will have no long-term consequences (e.g. Hoysak and Weatherhead 1991).…”

Section: Introductionmentioning

confidence: 99%

Long-term effects of repeated handling and bleeding in wild caught Great Tits Parus major

Oers

Carere

2007

J Ornithol

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Handling and bleeding are frequently used procedures in avian research and several studies show that they can exert short-term effects, such as elevation in corticosterone levels. However, the long-term effects of exposure to such manipulations are largely unknown, but could have important implications, especially for much of the long-term research on birds and experiments that involve longitudinal assessments. In this study, we evaluated the effect of handling and bleeding on some physiological and behavioural parameters. Hand-reared Great Tits Parus major originating from wild nests were used in two different experiments for other purposes. In these experiments, the birds were exposed to different frequencies of bleeding and handling events across a period of 45 days. The ''high stress'' group experienced a total of seven times handling and five times bleeding, while a ''low stress'' group was handled three times and bled only once. Thirty days after the experiments, when caught and handled from a cage, individuals of the high stress group were easier to catch, displayed significantly higher breath rates, and were more docile than individuals of the low stress group. No differences in body mass were detected. These results indicate that repeated manipulations cause evident long-term changes in coping with such procedures, which are likely due to learning effects, and provide empirical evidence that the past experimental history of an animal has to be taken into account in subsequent experiments.

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Sampling Blood from Birds: A Technique and an Assessment of Its Effect

Cited by 82 publications

References 23 publications

Experimental effects of early‐life corticosterone on the hypothalamic–pituitary–adrenal axis and pre‐migratory behaviour in a wild songbird

Experimental effects of early‐life corticosterone on the hypothalamic–pituitary–adrenal axis and pre‐migratory behaviour in a wild songbird

Capture and blood sampling do not affect foraging behaviour, breeding success and return rate of a large seabird: the black-browed albatross

Long-term effects of repeated handling and bleeding in wild caught Great Tits Parus major

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