Sample stacking for the analysis of penicillins by microemulsion electrokinetic capillary chromatography

Importance of Treatment Process on the Analysis of Penicillins in Milk Samples by Capillary Electrophoresis

“…Different background electrolytes have been described for PENs analysis in different matrices. The pH value used is in the interval from 3.6 to 9.2 using phosphate or borate and sodium dodecyl sulphate salts [24][25][26][27][28]. Different factors affecting the separation with the background electrolyte required evaluation.…”

Monitoring the On-line Concentration and Separation of Gold Nanoparticles Using the Reversed Electrode Polarity Stacking Mode and Micellar Electrokinetic Chromatography

“…In addition, I noticed that the peak widths of the signals in the electropherograms for both sizes of Au NPs were reduced slightly when the REPSM voltage processes were applied, suggesting that the Au NPs in the sample underwent stacking during the application of the REPSM; the finding is consistent with those of previous reports. 37,38 After completing the REPSM, the voltage was applied (positive polarity) for various MEKC time intervals and then pressure (50 mbar) was applied to mobilize the Au NP zones to the detector. Sets (a) and (b) in Fig.…”

Recent advances in the development and application of microemulsion EKC

“…Sample 'stacking' is a technique where improved separation efficiencies and detector response are obtained when using a sample diluent of lesser ionic strength than the microemulsion running buffer [31,67]. This technique has been used for the analysis of penicillins by MEEKC [68] and resulted in a 40-fold increase in detection limits. Sample stacking was used as an online preconcentration technique when analysing cephalosporins by MEEKC [69].…”

“…Recent methods to improve sensitivity and LODs have included sample stacking [68] to improve sensitivity up to 40-fold, and LIF detection (LIFD) [54,75], used for to achieve LODs of 5.3 and 3.9 ng/ml ephedrine and pseudoephedrine, respectively in Chinese herbal remedies [75].…”

“…5 shows the separation of ingredients in a cold medicine using a similar MEEKC method [88]. A sample stacking technique was used to analyse amoxicillin (a penicillin compound) in a commercial formulation by MEEKC [68], which showed up to a 40-fold increase in sensitivity and achieved LOD's of between 10 and 25 mg/ L. Furlanetto [27] …”