Monitoring the On-line Concentration and Separation of Gold Nanoparticles Using the Reversed Electrode Polarity Stacking Mode and Micellar Electrokinetic Chromatography

Liu, Fu‐Ken

doi:10.2116/analsci.26.1145

Cited by 16 publications

(10 citation statements)

References 40 publications

(37 reference statements)

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“…Also for the reason of compatibility with incubation settings, the use of surfactants was avoided in this study. Adding a charged surfactant to the running electrolyte is known as a factor contributing to an improved separation of differently sized gold nanoparticles; 15,16,21,22 however, this was beyond our present goals.…”

Section: Optimization Of Ce Conditionsmentioning

confidence: 87%

Speciation of metal-based nanomaterials in human serum characterized by capillary electrophoresis coupled to ICP-MS: a case study of gold nanoparticles

et al. 2015

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The development and optimization of a versatile analytical system for the speciation analysis of metal-containing nanoscale materials in blood serum is reported herein. Based on capillary electrophoresis (CE) interfaced with inductively coupled plasma mass spectrometry (ICP-MS), the method was shown to be feasible to investigate the interactions between serum proteins and gold nanoparticles of potential medicinal use, which are their first and foremost occurrence upon their entry into the circulatory system. To improve the separation resolution between the intact nanoparticles and different protein conjugates, the CE system was optimized with an emphasis on compatibility with physiological conditions, avoiding aggregation effects, and analyte recovery. Optimization allowed also for acquiring the acceptable figures of merit such as migration time and peak area precision of 1.0-6.4% and 2.4-6.9%, respectively, detection limits in the range of 0.8-1.0 μg L(-1) Au, and capillary recoveries on the order of 86-97%, depending on the nanoparticle size and conjugate type. We sytematically investigated the role of size in mediating protein adsorption to gold nanoparticles in a real-serum environment. At the initial stage of surface coating, the speciation of smaller particles (5 and 10 nm) was found to be dominated by albumin, transferrin (both in apo- and holo-form) playing the secondary role in developing the protein corona. For 20 and 50 nm nanoparticles, the contribution of transferrin is initially comparable; however, with time it becomes replaced by albumin. The time of attaining equilibrium adsorption is also a function of particle size but for the whole size range investigated, albumin is the only equilibrium binding partner. These principal findings prove that for metal-based nanomaterials in general, serum protein conjugates could be variable in composition depending on the protein abundance and binding affinity, as well as the residence time in the bloodstream.

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Section: Optimization Of Ce Conditionsmentioning

confidence: 87%

Speciation of metal-based nanomaterials in human serum characterized by capillary electrophoresis coupled to ICP-MS: a case study of gold nanoparticles

et al. 2015

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“…These tools provide much information about factor effects permitting empirical predictions of peak resolutions with a few experiments (ca. [15][16][17][18][19][20], which are performed in random to prevent systematic error. Not only the number of experiments can be reduced but the effects of interaction of factors can also be inferred.…”

Section: Use Of Other Approaches: 2d Separations and Chemometricsmentioning

confidence: 99%

Micellar electrokinetic chromatography: A review of methodological and instrumental innovations focusing on practical aspects

Silva

2012

Electrophoresis

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This review article addresses recent methodological and instrumental innovations in MEKC with emphasis on practical aspects. Like its predecessors, this review is intended to provide an updated overview covering work on the most salient methodological contributions to enhancing sensitivity and resolution in MEKC-based determinations published over the past two years. The most widespread approaches to enhancing sensitivity, which include improving "classical" online sample concentration techniques, combinations of on- and off-line sample concentration protocols and recent developments are discussed, and so are modifications of existing MEKC systems with various micellar phases, the use of BGE additives (organic modifiers, chiral selectors, gold nanoparticles) and coated capillaries, and the implementation of 2D separations and chemometric methods to enhance resolution. Instrumental approaches such as MS and LIF are also discussed, and proposals for overcoming the problems typically encountered in directly coupling MEKC with MS, and the recent inception of quantum dots with a great potential for LIF detection in MEKC, are also dealt with. Finally, foreseeable developments on potential future directions are also expressed.

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“…[36][37][38][39] Up to 500-fold enrichment factors might be important for analyzing real biofluid samples containing trace concentrations of nanoparticles.…”

Section: Application Of Ce To Analyzing Nanoparticle-protein Interactmentioning

confidence: 99%

Interactions of tumour-targeting nanoparticles with proteins: potential of using capillary electrophoresis as a direct probe

et al. 2012

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Metal-based nanoscale particles possess unique optoelectronic or magnetic properties that make them highly promising as imaging agents in cancer therapy research. The fate of nanoparticles in vivo and particularly, the delivery to tumours are closely related to their interactions with plasma proteins. Furthermore, proteins can be used to modify the nanoparticle surface in order to facilitate active targeting to tumours. Therefore, there is an ongoing need for new and more capable analytical methodologies to characterize the protein-nanoparticle binding. Due to the small-sample volume requirement, high degree of resolution and, most importantly, mild, species-friendly separation conditions, capillary electrophoresis (CE) is gaining increasing popularity in the analysis of protein-nanoparticle interaction. This perspective article highlights the potential of CE in studying reactions associated with protein-mediated transformations of nanoparticles, with the focus on quantum dots, gold and iron oxide nanoparticles. Different ways by which CE can be applied to such monitoring are summarized and critically assessed using a representative coverage of recent publications.

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Monitoring the On-line Concentration and Separation of Gold Nanoparticles Using the Reversed Electrode Polarity Stacking Mode and Micellar Electrokinetic Chromatography

Cited by 16 publications

References 40 publications

Speciation of metal-based nanomaterials in human serum characterized by capillary electrophoresis coupled to ICP-MS: a case study of gold nanoparticles

Speciation of metal-based nanomaterials in human serum characterized by capillary electrophoresis coupled to ICP-MS: a case study of gold nanoparticles

Micellar electrokinetic chromatography: A review of methodological and instrumental innovations focusing on practical aspects

Interactions of tumour-targeting nanoparticles with proteins: potential of using capillary electrophoresis as a direct probe

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