Sample preparation technique and white cell content influence the detectable levels of growth factors in platelet concentrates

Zimmermann, Robert; Arnold, Don W.; Strasser, Erwin; Ringwald, Juergen; Schlegel, August Wilhelm von; Wiltfang, Joerg; Eckstein, Reinhold

doi:10.1111/j.0042-9007.2003.00361.x

Cited by 133 publications

(154 citation statements)

References 44 publications

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“…Platelet-derived growth factor concentrations have also been investigated by several other laboratories (Christgau et al, 2006;Cognasse et al, 2006;Doucet et al, 2005;Frechette et al, 2005;Gruber et al, 2002;Kaps et al, 2002;lacoste et al, 2003;Ogino et al, 2006;Schallmoser et al, 2007;Shen et al, 2006;Weibrich et al, 2002;Zimmermann et al, 2003). the results of these analyses are not comparable with our results, because of different preparation methods: All except el Backly et al and Gruber et al (el Backly et al, 2011;Gruber et al, 2002) stored platelets in serum, which also contains growth factors, and/or in platelet additive solution.…”

Section: Stimulation Of Erk1/2 Map Kinasescontrasting

confidence: 69%

Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media

Rauch

2011

ALTEX

177

149

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Section: Stimulation Of Erk1/2 Map Kinasescontrasting

confidence: 69%

Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media

Rauch

2011

ALTEX

177

149

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“…The syringes were then frozen at −80°C and thawed at 37°C 3 times (Zimmermann et al, 2003) to allow the release of platelet-derived factors. The PC was subjected to bacteriological examination to verify its sterility.…”

Section: Preparation Of Platelet Concentratementioning

confidence: 99%

Intramammary administration of platelet concentrate as an unconventional therapy in bovine mastitis: First clinical application

Consiglio

Spelta

Garlappi³

et al. 2014

Journal of Dairy Science

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Bovine udder infections induce a variety of changes in gene expression of different growth factors that may suggest their possible role in glandular tissue protection or repair processes. Growth factors and also chemokines and cytokines may act synergistically to increase the infiltration of neutrophils and macrophages to promote angiogenesis, fibroplasia, matrix deposition, and, ultimately, re-epithelialization. Considering the vast applications, typically in human medicine, of platelet concentrate (PC) and its ease of preparation, the aim of our study was to evaluate an alternative therapy to stimulate the regeneration of glandular tissue, administering a concentration in excess of the growth factors contained in the PC. In each one of the 3 farms examined in the trial, PC was prepared from donor cows in good health, free from infections, and with no records of medications administered during the previous 2 mo. The platelet produced in one farm was used only for treating the cows of the same farm in a heterologous way. A total of 229 mastitic quarters were divided in 3 groups: antibiotic group (treated with intramammary antibiotic), antibiotic and PC group (treated intramammarily with antibiotics in association with PC), and PC group (treated with intramammary PC alone). The diagnosis of mastitis was based on somatic cell count and bacteriological evaluation of the milk from the affected quarter. Platelet concentrate, alone or in association with antibiotic, was used for 3 consecutive days as an unconventional therapy in bovine acute and chronic mastitis. Our data show that the associated action of antibiotic and PC performed significantly better than the antibiotic alone, either for the recovery of the affected mammary quarters or for somatic cell count reduction. In the same way, the association antibiotic plus PC showed significantly fewer relapses compared with the antibiotic alone, either for acute or chronic mastitis. The treatment with only PC did not show statistically significant differences compared with both antibiotic alone or associated treatment for acute mastitis, and it was better than the use of only antibiotic for chronic mastitis. Our results show that PC alone may be useful for a quick resolution of the inflammatory response, playing a role in limiting the tissue damage to the mammary gland parenchyma and reducing the recurrence rates.

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“…Nevertheless, it is doubtful that the clotting process releases all PLT-derived GFs into the serum. Recently we showed that PLT destruction by the detergent Triton X-100 causes the release of much more GFs from PLTs into the supernatant than do in vitro clotting processes (25 ). In addition, Tezono et al (26 ) recently demonstrated that VEGF is partially trapped within fibrin clots.…”

mentioning

confidence: 97%

Effect of Specimen Anticoagulation on the Measurement of Circulating Platelet-Derived Growth Factors

et al. 2005

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found seasonal variation (27 ), and simple and noninvasive sample collection. In addition to 24-h samples, as used in this study, OC can be measured from spot urine samples collected as the first or second morning void (9, 11 ). Moreover, U-OC responds to therapy more rapidly than S-OC.In summary, the results suggest that U-OC is a useful marker for monitoring short-term changes in bone metabolism in response to antiresorptive therapy in postmenopausal women.

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Sample preparation technique and white cell content influence the detectable levels of growth factors in platelet concentrates

Cited by 133 publications

References 44 publications

Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media

Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media

Intramammary administration of platelet concentrate as an unconventional therapy in bovine mastitis: First clinical application

Effect of Specimen Anticoagulation on the Measurement of Circulating Platelet-Derived Growth Factors

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