Sample Preparation for Detection of Different Bacterial Strains by Matrix‐Assisted Laser Desorption/Ionization Time‐of‐Flight (MALDI‐TOF) Mass Spectrometry

Chac, Denise; Kordahi, Melissa C.; Brettner, Leandra; DePaolo, R. William

doi:10.1002/cpz1.212

Cited by 3 publications

(1 citation statement)

References 18 publications

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“…MALDI TOF was carried out using Bruker MALDI-TOF (Ultra eXtreme II). It was done to identify variations in protein caused by metal exposure (Chac et al 2021) Bacterial culture was centrifuged and sonicated. Metallothionein would be present in the supernatant.…”

Section: Mechanism Of Heavy Metal Reductionmentioning

confidence: 99%

The comprehension of bioremediation for the isolation and characterization of heavy metal tolerant Aeromonas sanarellii bacteria in the wastewater-contaminated soil

Aravind,

Sivaji,

Nagaraj

et al. 2023

Preprint

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The large number of toxic metals accumulate in water and soil environment as a result of unmanaged industrial waste disposal, significantly impacting a variety of health-related issues. Heavy metal resistance is very high among microorganisms in the soil's rhizosphere. In this work, we isolated such naturally existing rhizosphere bacteria Aeromonas sanarellii (A. sanarellii) from heavily contaminated soil in the Anakaputhur area, southern part of Chennai city, India. On the basis of the partial amplification of 16S rRNA using new primers, A. sanarellii was molecularly identified. Then, 33 strains were used to align the sequence in order to study phylogenetic relationships using the neighbour joining method. This study assessed its ability of reducing and detoxifying heavy metals based on the results of subsequent testing. The effective concentration (EC50) and the internationalization of heavy metals were determined using spectrophotometric method. The structural changes in tolerant bacteria were studied using Scanning electron microscope (SEM). Protein profiling was performed using SDS PAGE and MALDI TOF analysis. Amount of metallothionein produced upon exposure was measured by using UV-Vis spectrophotometer. This study found that, as a consequence, selecting A. sanarellii based on their heavy metal resistance and biodegradation ability could serve as a starting point for developing potential bioremediation agent for toxic effluent treatment technology.

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Section: Mechanism Of Heavy Metal Reductionmentioning

confidence: 99%

The comprehension of bioremediation for the isolation and characterization of heavy metal tolerant Aeromonas sanarellii bacteria in the wastewater-contaminated soil

Aravind,

Sivaji,

Nagaraj

et al. 2023

Preprint

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Genomic and functional characterization of a mucosal symbiont involved in early-stage colorectal cancer

Kordahi

Stanaway

Avril

et al. 2021

Cell Host & Microbe

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Polyunsaturated fatty acid arachidonic acid increases the virulence of gut pathogen, Yersinia enterocolitica

Chac

Crebs

Yee

et al. 2021

Preprint

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Food-borne illnesses are a major health concern worldwide. While 1 in 6 individuals are infected in the United States yearly, there is little research into which dietary factors can alter the risk of infection. Despite evidence suggesting a correlation between obesity and enteric infection, the few reported studies focus on the role of dietary factors and the impact on host tissues and susceptibility. The direct impact of dietary constituents on the virulence of a pathogen has largely been ignored. One component of the Western diet that has been correlated with increasing inflammatory diseases is increased consumption of omega-6 polyunsaturated fatty acids such as arachidonic acid. Here, we show that arachidonic acid directly alters the pathogenicity of the food-borne pathogen Yersinia enterocolitica. Using in vitro cellular adherence assays, proteomic peptide mass fingerprint profiles and in vivo mouse models, we show that arachidonic acid can alter the pathogenesis of Y. enterocolitica by increasing proliferation and intracellular invasion. These findings have major implications in more than food safety, potentially revealing how current dietary habits may increase the virulence of food-borne pathogens.

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Sample Preparation for Detection of Different Bacterial Strains by Matrix‐Assisted Laser Desorption/Ionization Time‐of‐Flight (MALDI‐TOF) Mass Spectrometry

Cited by 3 publications

References 18 publications

The comprehension of bioremediation for the isolation and characterization of heavy metal tolerant Aeromonas sanarellii bacteria in the wastewater-contaminated soil

The comprehension of bioremediation for the isolation and characterization of heavy metal tolerant Aeromonas sanarellii bacteria in the wastewater-contaminated soil

Genomic and functional characterization of a mucosal symbiont involved in early-stage colorectal cancer

Polyunsaturated fatty acid arachidonic acid increases the virulence of gut pathogen, Yersinia enterocolitica

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