Sample enrichment techniques in capillary electrophoresis: Focus on peptides and proteins

Monton, Maria Rowena N.; Terabe, Shigeru

doi:10.1016/j.jchromb.2006.04.017

Cited by 33 publications

(24 citation statements)

References 108 publications

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“…Our proposed methods with HEC-g-P4VP copolymer coatings have been tested using a complex real world sample, human saliva, and employing a field effect sample stacking, which is a very important concentration technique widely used in CE [40][41][42][43]. Analytes can be automatically stacked after they cross the concentration boundary.…”

Section: Analysis Of Biological Samplesmentioning

confidence: 99%

Hydroxyethylcellulose‐graft‐poly (4‐vinylpyridine) as a novel, adsorbed coating for protein separation by CE

2009

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A novel graft copolymer, hydroxyethylcellulose-graft-poly (4-vinylpyridine), used as a physically adsorbed coating of the fused-silica (FS) capillaries, was synthesized by using ceric ammonium nitrate initiator in aqueous nitric acid solution. EOF measurements showed that the above graft copolymer coating of the FS capillaries could suppress EOF effectively compared with the bare FS capillaries. The adsorbed coating exhibited minimal interactions with proteins, providing efficient protein separations with excellent repeatability. Interestingly, the electrical charge of the coated capillary wall could be modulated by varying the pH of the running buffer, which makes possible the analysis of basic and acidic proteins in the same capillary. The effects of pH on suppressing EOF and protein separation were investigated in detail. The results demonstrated that the hydroxyethylcellulose-graft-poly (4-vinylpyridine) copolymer coatings have great potential in the diagnosis and proteomics.

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Section: Analysis Of Biological Samplesmentioning

confidence: 99%

Hydroxyethylcellulose‐graft‐poly (4‐vinylpyridine) as a novel, adsorbed coating for protein separation by CE

2009

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“…Techniques for sample enrichment in CE including protein samples have been reviewed [8,9]. Recently, methods based on protein adsorption, electric field stacking and ITP have been used to concentrate proteins.…”

Section: Preconcentrationmentioning

confidence: 99%

Capillary electrophoresis of proteins 2005–2007

Dolnı́k¹

2007

Electrophoresis

152

106

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This review article with 239 references describes recent developments in capillary electrophoresis of proteins, and covers the two years since the previous review (V. Dolník, Electrophoresis 2006, 27, 126-141) through spring 2007. It includes topics related to CE of proteins, such as sample pretreatment, wall coatings, improving separation, various forms of detection, and special electrophoretic techniques including ACE, CIEF, capillary ITP, and CEC. The paper describes applications of CE to analysis of proteins in real-world samples including human body fluids, food and agricultural samples, protein pharmaceuticals and recombinant protein preparations.

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“…To accomplish both a suitable resolution and sensitivity, the analytes must experience at a given location a sudden decrease in their mobility in order to be concentrated in a zone sharper than the initial sample zone. Different mechanisms can be used to induce this concentration process [2][3][4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Electrokinetic supercharging for highly efficient peptide preconcentration in capillary zone electrophoresis

2008

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Electrokinetic supercharging has been integrated in CZE for the development of a highly sensitive methodology for protein tryptic digest analysis. A careful choice of the experimental conditions led to sensitivity enhancement factors between 1000 and 10 000 whilst maintaining a satisfactory resolution. Peptides in the low nanomolar concentration range have been detected despite the use of the poorly sensitive UV absorbance detection mode. The buffer system used in this study is fully suitable for coupling CE to MS.

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Sample enrichment techniques in capillary electrophoresis: Focus on peptides and proteins

Cited by 33 publications

References 108 publications

Hydroxyethylcellulose‐graft‐poly (4‐vinylpyridine) as a novel, adsorbed coating for protein separation by CE

Hydroxyethylcellulose‐graft‐poly (4‐vinylpyridine) as a novel, adsorbed coating for protein separation by CE

Capillary electrophoresis of proteins 2005–2007

Electrokinetic supercharging for highly efficient peptide preconcentration in capillary zone electrophoresis

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