Sample concentration in a microfluidic paper-based analytical device using ion concentration polarization

Phan, Dinh‐Tuan; Shaegh, Seyed Ali Mousavi; Yang, Chun; Nguyen, Nam‐Trung

doi:10.1016/j.snb.2015.08.127

Cited by 89 publications

(71 citation statements)

References 26 publications

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“…In the region where the enriched ion concentration is obtained; the average grey scale value of the fluorescent intensity profile reaches 90 before the electric field is turned on and the grey scale reaches 122 after the electric field is turned on, we obtain a 35.6% amplification in the fluorescent signal for the ions in the water phase of the O/W emulsion which correlates to 100% enrichment of the dye concentration in the water phase of the O/W emulsion. This enrichment factor is similar to the values reported by other researchers .…”

Section: Resultssupporting

confidence: 92%

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A multi‐module microfluidic platform for continuous pre‐concentration of water‐soluble ions and separation of oil droplets from oil‐in‐water (O/W) emulsions using a DC‐biased AC electrokinetic technique

et al. 2017

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A novel continuous flow microfluidic platform specifically designed for environmental monitoring of O/W emulsions during an aftermath of oil spills is reported herein. Ionized polycyclic aromatic hydrocarbons which are toxic are readily released from crude oil to the surrounding water phase through the smaller oil droplets with enhanced surface area. Hence, a multi-module microfluidic device is fabricated to form ion enrichment zones in the water phase of O/W emulsions for the ease of detection and to separate micron-sized oil droplets from the O/W emulsions. Fluorescein ions in the water phase are used to simulate the presence of these toxic ions in the O/W emulsion. A DC-biased AC electric field is employed in both modules. In the first module, a nanoporous Nafion membrane is used for activating the concentration polarization effect on the fluorescein ions, resulting in the formation of stable ion enrichment zones in the water phase of the emulsion. A 35.6% amplification of the fluorescent signal is achieved in the ion enrichment zone; corresponding to 100% enrichment of the fluorescent dye concentration. In this module, the main inlet is split into two channels by using a Y-junction so that there are two outlets for the oil droplets. The second module located downstream of the first module consists of two oil droplet entrapment zones at two outlets. By switching on the appropriate electrodes, either one of the two oil droplet entrapment zones can be activated and the droplets can be blocked in the corresponding outlet.

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Section: Resultssupporting

confidence: 92%

“…Once the ICP process is stabilized, ion enrichment zones are induced in those regions. This phenomena has been utilized for a wide range of applications such as separation, concentration , desalination , mixing , etc. of target analyte species.…”

Section: Methodsmentioning

confidence: 99%

A multi‐module microfluidic platform for continuous pre‐concentration of water‐soluble ions and separation of oil droplets from oil‐in‐water (O/W) emulsions using a DC‐biased AC electrokinetic technique

et al. 2017

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“…The most commonly used method is ion concentration polarization (ICP) [20][21][22][23]. This can provide concentration factors of 100-1000 in 2-10 minutes after filling, but require nanoporous membranes, electrodes and relatively high voltages.…”

Section: Introductionmentioning

confidence: 99%

Rapid evaporation-driven chemical pre-concentration and separation on paper

Syms

2017

Biomicrofluidics

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Airflow-enhanced evaporation is investigated as a method for rapid chemical pre-concentration on a thin porous substrate. The mechanism is described by combining 1D models of capillary rise, chromatography and pervaporation concentration. It is shown that the effective length of the column can be shorter than its actual length, allowing concentrate to be held at a stagnation point and then released for separation, and that the Péclet number, which determines concentration performance, is determined only by the substrate properties. The differential equations are solved dynamically, and it is shown that faster concentration can be achieved during capillary filling.Experiments are carried out using chromatography paper in a ducted airflow and concentration is quantified by optical imaging of water-soluble food dyes. Good agreement with the model is obtained, and concentration factors of ≈ 100 are achieved in 10 mins using Brilliant Blue FCF.Partial separation of Brilliant Blue from Tartrazine is demonstrated immediately following concentration, on a single un-patterned substrate. The mechanism may provide a method for improving the sensitivity of lab-on-paper devices.

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“…ICP, an electrokinetic phenomenon that enables amplification or concentration of charged particles/ions into several orders of magnitude, has been studied by several researchers for its characteristics such as non‐linear electrokinetic flow, over limiting current, ion‐depletion zone, micro vortices under different conditions such as electrothermal convection, natural convection, chemical reaction, and charge regulation . The phenomenon has employed in concentrating analytes, proteins, viruses, and other fluorescent dyes on µPADs by multiple researchers . Yeh et al observed a concentration factor of 944 folds for the fluorescein and 835 folds for FITC‐BSA .…”

Section: Introductionmentioning

confidence: 99%

Rapid pre‐concentration of Escherichia coli in a microfluidic paper‐based device using ion concentration polarization

et al. 2019

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We report a microfluidic paper based analytical device implementing ion concentration polarization (ICP) for rapid pre-concentration of Escherichia coli in water. The fabricated device consists of a paper channel with a Nafion ® membrane and in-built micro wire electrodes to supply electric voltage to induce the ICP effect. E. coli cells were stained with SYTO 9 and fluorescence was used as a sensing method. The device achieved high concentration factor up to 2 × 10 5 within minutes. The effect of total ion concentration, on ICP and fluorescence intensity was studied. The reported device and method are suitable and effective for detection of E. coli during ballast water quality monitoring, coastal water quality monitoring where high salinity water is present.

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Sample concentration in a microfluidic paper-based analytical device using ion concentration polarization

Cited by 89 publications

References 26 publications

A multi‐module microfluidic platform for continuous pre‐concentration of water‐soluble ions and separation of oil droplets from oil‐in‐water (O/W) emulsions using a DC‐biased AC electrokinetic technique

A multi‐module microfluidic platform for continuous pre‐concentration of water‐soluble ions and separation of oil droplets from oil‐in‐water (O/W) emulsions using a DC‐biased AC electrokinetic technique

Rapid evaporation-driven chemical pre-concentration and separation on paper

Rapid pre‐concentration of Escherichia coli in a microfluidic paper‐based device using ion concentration polarization

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