Sambulin A and B, non-glycosidic iridoids from Sambucus ebulus, exert significant in vitro anti-inflammatory activity in LPS-induced RAW 264.7 macrophages via inhibition of MAPKs's phosphorylation

Balkan, İrem Atay; Akülke, Ayca Zeynep İlter; Bağatur, Yeşim; Telci, Dilek; Gören, Ahmet; Kırmızıbekmez, Hasan; Yeşilada, Erdem

doi:10.1016/j.jep.2017.06.002

Cited by 14 publications

(7 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, it is necessary to identify which phytochemical in the natural resources contributes to the anti-inflammatory effect. Various phytochemicals, especially flavonoids and terpenoids, have been found to be anti-inflammatory compounds and their anti-inflammatory mechanisms have also been clarified [ 21 , 22 , 23 , 24 , 25 , 26 ]. Among them, the iridoids attracted much attention and some iridoid compounds have been proven to be of anti-inflammatory [ 27 , 28 ].…”

Section: Discussionmentioning

confidence: 99%

Scandoside Exerts Anti-Inflammatory Effect Via Suppressing NF-κB and MAPK Signaling Pathways in LPS-Induced RAW 264.7 Macrophages

Liu

et al. 2018

IJMS

View full text Add to dashboard Cite

The iridoids of Hedyotis diffusa Willd play an important role in the anti-inflammatory process, but the specific iridoid with anti-inflammatory effect and its mechanism has not be thoroughly studied. An iridoid compound named scandoside (SCA) was isolated from H. diffusa and its anti-inflammatory effect was investigated in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. Its anti-inflammatory mechanism was confirmed by in intro experiments and molecular docking analyses. As results, SCA significantly decreased the productions of nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) and inhibited the levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), TNF-α and IL-6 messenger RNA (mRNA) expression in LPS-induced RAW 264.7 macrophages. SCA treatment suppressed the phosphorylation of inhibitor of nuclear transcription factor kappa-B alpaha (IκB-α), p38, extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK). The docking data suggested that SCA had great binding abilities to COX-2, iNOS and IκB. Taken together, the results indicated that the anti-inflammatory effect of SCA is due to inhibition of pro-inflammatory cytokines and mediators via suppressing the nuclear transcription factor kappa-B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways, which provided useful information for its application and development.

show abstract

Section: Discussionmentioning

confidence: 99%

Scandoside Exerts Anti-Inflammatory Effect Via Suppressing NF-κB and MAPK Signaling Pathways in LPS-Induced RAW 264.7 Macrophages

Liu

et al. 2018

IJMS

View full text Add to dashboard Cite

show abstract

“…The use of herbal plants in pharmaceutical research and the drug's development are of uttermost importance . Although the anti‐inflammatory, antioxidant, anti‐ Helicobacter pylori and antigiardial activities of S ebulus extract have previously been reported, the antileishmanial activity has not been described yet. Hence, to confirm the claimed traditional administration of S ebulus in Iranian traditional medicine (Persian traditional medicine) as a major source for antioxidants, the current work is the first comprehensive study aiming to examine the main therapeutic effects of S ebulus aqueous extract as an antiparasitic herbal drug with the plausible immunoregulation of immune cells without excessive toxicity to the host cells and direct antileishmanial activities in in vitro and mice model.…”

Section: Discussionmentioning

confidence: 95%

“…S ebulus plant is known as Khamaan in Iranian (Persian) traditional medicine which is prescribed as a natural herbal remedy in several diseases and various painful conditions such as sore throat, arthritis, honey bee bites and haemorrhoid . Further, this herbal plant has lately shown to have anti‐ Helicobacter pylori activity, antitumour, antinociceptive, anti‐inflammatory, anti‐angiogenic and anti‐oxidative activities . Previous studies have revealed that S ebulus has different active compounds including glycosides flavonoids, tannins, steroids, colchicine, ebulitins, anthocyanins, caffeic acids and volatile substances .…”

Section: Introductionmentioning

confidence: 99%

Sambucus ebulus extract stimulates cellular responses in cutaneous leishmaniasis

Heidari‐Kharaji

Omrani

Badirzadeh

et al. 2018

Parasite Immunology

View full text Add to dashboard Cite

Summary This is the first study aiming to determine the therapeutic effects of the Sambucus ebulus aquatic extract as an antileishmanial herbal drug and evaluate the immune responses in Leishmania major major infected BALB/c mice. The antileishmanial activity of S ebulus aquatic extract was evaluated using MTT test as well as parasite rescue and transformation assay. Footpad swelling and parasite load of infected mice were measured by several techniques. The immune responses were evaluated by measuring the levels of IFN‐γ, IL‐4, nitric oxide and arginase. The results indicated that S. ebulus can significantly decrease L. major promastigotes and amastigotes viability, but it was not toxic to macrophages. The lesion size, parasite burden and the level of ARG decreased in the treated infected mice, while the IFN‐γ‐to‐IL‐4 ratio and the level of NO increased significantly. Altogether, the S. ebulus extract is an effective compound for killing Leishmania parasite without excessive toxicity to the host cells and can cure the CL by switching the host immune responses towards Th1 response. Thus, it may be a perfect therapeutic option for CL treatment.

show abstract

“…The nitrite accumulated in the culture medium was measured by Griess assay. The nitrite concentration in the samples was calculated from the standard curve of sodium nitrite [24] .…”

Section: In Vitro Antiinflammatory Activitymentioning

confidence: 99%

Characterization of Phenolic Constituents and Pharmacological Activity of Achillea vermicularis

Taşkın¹,

Balkan²

2019

pharmaceutical-sciences

View full text Add to dashboard Cite

Taskin et al.: Biological activity of Achillea vermicularis The aim of the present study was to compare for the first time, the antiinflammatory, antioxidant and antiurease activities of 15 different extracts of Achillea vermicularis, which were obtained from three extraction methods using five different solvents. Phenolic contents in chloroform extract showed strong antiinflammatory and antioxidant activity and were analysed by high-performance liquid chromatography with diode-array detection and confirmed by liquid chromatography quadrupole time-of-flight mass spectrometry. The active phenolic compounds in chloroform extracts were identified as chlorogenic acid, caffeic acid, rutin, dicaffeoylquinic acid, naringenin, quercetagetin 3,6-dimethyl ether, and 8-hydroxysalvigenin. All these compounds except caffeic acid were detected for the first time in Achillea vermicularis. These results indicated that chloroform extract of the plant possessed a high potential to be used as a natural source in food and pharmaceutical industry.

show abstract

Sambulin A and B, non-glycosidic iridoids from Sambucus ebulus, exert significant in vitro anti-inflammatory activity in LPS-induced RAW 264.7 macrophages via inhibition of MAPKs's phosphorylation

Cited by 14 publications

References 37 publications

Scandoside Exerts Anti-Inflammatory Effect Via Suppressing NF-κB and MAPK Signaling Pathways in LPS-Induced RAW 264.7 Macrophages

Scandoside Exerts Anti-Inflammatory Effect Via Suppressing NF-κB and MAPK Signaling Pathways in LPS-Induced RAW 264.7 Macrophages

Sambucus ebulus extract stimulates cellular responses in cutaneous leishmaniasis

Characterization of Phenolic Constituents and Pharmacological Activity of Achillea vermicularis

Contact Info

Product

Resources

About