Salvinia auriculata: chemical profile and biological activity against Staphylococcus aureus isolated from bovine mastitis

Abstract

The aquatic plant Salvinia auriculata has been shown to possess promising properties for the treatment of Staphylococcus aureus bovine mastitis. The disease affects cattle health and compromises dairy cattle productivity, resulting in reduced milk production and higher mortality rates. The aim of this study was to evaluate the antimicrobial activity, antibiofilm activity, and toxicity of S. auriculata root extracts using bovine mammary epithelial cells (MAC-T); determine the chemical composition of the most active extract; and develop an S. auriculata antiseptic solution for pre-and post-milking teat disinfection. Plants were collected during the four seasons of the year. The most active hexane extract was subjected to bioguided fractionation, which resulted in the isolation of six known compounds, stigmast-22-ene-3,6-dione, stigmasterol, friedelinol, β-sitosterol, octadecyl alcohol, and octadecanoic acid. The antimicrobial and antibiofilm activities of the most active extract and isolated compounds were determined against nine S. aureus strains isolated from cows with mastitis. The efficacy of the S. auriculata teat dip formulation was tested using an excised teat model (ex vivo), and promising results were obtained. The S. auriculata extract formulation proved to be as effective as commercial antimicrobials in reducing log counts in excised teats.

Estudos e tendências atuais em Ciências Ambientais e Agrarias

“…auriculata, óleo de canela, nanopartículas de prata (AgNPs), uso de AINEs (Antiinflamatórios não-esteroidais), terapia nutricional, homeopatia, seleção genética, uso de outras ervas, entre outros (EL-AZIZ et al, 2021;PURGATO, 2021;SHARUN, 2021).…”

Biofilme Estafilocócico: uma fonte de prejuízos para a pecuária leiteira na região agreste de Pernambuco, Brasil

“…No entanto, a pecuária leiteira tem como principal desafio a mastite bovina, infecção da glândula mamária, que tem impactado negativamente no setor, devido a diminuição na produção de leite, redução na qualidade do leite e derivados, além de gastos com medicação, tratamento e descarte do animal (PURGATO et al, 2021).…”

Natural products isolation studies of native Australian fern species

“…[1] Bioactivity-guided phytochemical investigation of these two Australian species allowed for the isolation of more than sixty different secondary metabolites that include diterpenes, polyphenols, fatty acids, triterpene, apocarotenoids, acyclic sesquiterpenoids, monoterpenes, jasmonates, steroids and coumarins. [50][51][52] However, a large number of native Australian ferns, particularly endemic species, have not been the subject of natural products isolation studies.…”