Salvianolic Acid B Maintained Stem Cell Pluripotency and Increased Proliferation Rate by Activating Jak2–Stat3 Combined with EGFR–Erk1/2 Pathways

Liu, Chia Hui; Shyu, Woei Cherng; Fu, Ru Huei; Huang, Shyh Jer; Chang, Cheng Hsuan; Huang, Yu Chuen; Chen, Shih Yin; Lin, Shinn Zong; Liu, Shih‐Ping

doi:10.3727/096368914x678391

Cited by 10 publications

(4 citation statements)

References 30 publications

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“…To date, there were several strategies to enhance the reprogramming efficiency, including knockdown of p53 gene 9 , hypoxic conditions 10,11 , epigenetic modification 12 , regulation of microRNAs 13 and addition of small molecular compounds 14,15 . In 2003, one group reported a near 100% reprogramming efficiency in mouse and human cells via OKSM transduction and Mbd3 depletion 16 .…”

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confidence: 99%

The novel application of cordycepin in maintaining stem cell pluripotency and increasing iPS cell generation efficiency

Wang

Chang

Lin

et al. 2020

Sci Rep

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Maintaining the pluripotency of either embryonic stem (eS) cells or induced pluripotent stem (ipS) cells is a fundamental part of stem cell research. in this study, we reported that cordycepin promoted the expression of pluripotency markers in eS and ipS cells. eS cells treated with cordycepin demonstratedtheir potential for generating embryoid bodies and differentiating into all three germ layers. The expression levels of phospho-Jak2, phospho-Stat3, integrin αV, and integrin β5 were increased after cordycepin treatment. Furthermore, the protein expression levels of IL-6 family proteins (IL-6, IL-11, Lif, oncostatin M (oSM), ciliary neurotrophic factor (cntf)), and epidermal growth factor (eGf) were also upregulated after cordycepin treatment, but were restored after co-treatment with a Jak2 inhibitor (AG490). The gene expression levels of Yamanaka factors were upregulated in mouse embryonic fibroblasts (MEFs) after cordycepin treatment. Moreover, the generation efficiencies of iPS cells were elevated after cordycepin treatment. We found that ipS cells generated after cordycepin treatment, not only expressed pluripotency markers, but also showed the ability of differentiating into neuron stem/ progenitor cells. taken together, we demonstrated that cordycepin maintained the pluripotency of stem cells via regulation of extracellular matrix (ECM) and Jak2/Stat3 signaling pathway and improved the generation efficiency of iPSCs.

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confidence: 99%

The novel application of cordycepin in maintaining stem cell pluripotency and increasing iPS cell generation efficiency

Wang

Chang

Lin

et al. 2020

Sci Rep

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“…The present study indicated that salvianolic acids were the most water-soluble in Salvia miltiorrhiza Bunge (14). Salvianolic acid B (Fig.…”

Section: Introductionmentioning

confidence: 51%

“…The current study demonstrated that salvianolic acid B significantly upregulated p-STAT3 protein expression in CSVD rat. Liu et al (14) reported that salvianolic acid B increased proliferation rate by activating Janus kinase 2-STAT3 pathways in stem cells.…”

Section: Discussionmentioning

confidence: 99%

Salvianolic acidï¿½B recovers cognitive deficits and angiogenesis in a cerebral small vessel disease rat model via the STAT3/VEGF signaling pathway

Wang

2017

Mol Med Report

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Salvianolic acid B is one of the key water‑soluble components of Salvia extract. It has been verified that salvianolic acid B possesses multiple pharmacological activities, it protects against myocardial infarction, however additionally improves injury of myocardial ischemia‑reperfusion. The present study, the possible effects of salvianolic acid B on cognitive deficits and angiogenesis in cerebral small vessel disease were investigated. Salvianolic acid B was identified to recover cognitive deficits and neurocytes, reduce inflammation, oxidative stress and neurocyte apoptosis (caspase‑3 and Bax protein expression) in cerebral small vessel disease rats. In addition, salvianolic acid B upregulated signal transducer and activator of transcription 3 (STAT3) phosphorylation protein expression, and induced vascular endothelial growth factor (VEGF) and VEGF receptor 2 protein expression in cerebral small vessel disease rats. In conclusion, the results demonstrated that salvianolic acid B recovers cognitive deficits and angiogenesis in the cerebral small vessel disease rat model via STAT3/VEGF signaling pathway.

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“…This expense has limited the number of labs that can participate in stem cell research and has encouraged efforts to find a viable alternative. Several studies have shown that dimethyl sulfoxide (DMSO) ( Yi et al, 2020 ), salvianolic acid B ( Liu et al, 2014 ), and cordycepin ( Wang et al, 2020 ) could maintain the expression of pluripotency markers in mESCs cultured in the absence of LIF; however, the developmental pluripotency of these mESCs was elusive.…”

Section: Discussionmentioning

confidence: 99%

Oncostatin M Maintains Naïve Pluripotency of mESCs by Tetraploid Embryo Complementation (TEC) Assay

Tian

Liu

et al. 2021

Front. Cell Dev. Biol.

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It has been well established that leukemia inhibitory factor (LIF) is essential for maintaining naïve pluripotency of embryonic stem cells (ESCs). Oncostatin M (OSM) is a member of the IL-6 family of cytokines which share gp130 as a receptor subunit, and the OSM-gp130 complex can recruit either LIF receptor β or OSM receptor β. Here we show that OSM can completely replace LIF to maintain naïve pluripotency of ESCs. Mouse ESCs (mESCs) cultured in the presence of LIF or OSM not only express pluripotency genes at similar levels but also exhibit the same developmental pluripotency as evidenced by the generation of germline competent chimeras, supporting previous findings. Moreover, we demonstrate by tetraploid embryo complementation assay, the most stringent functional test of authentic pluripotency that mESCs cultured in OSM produce viable all-ESC pups. Furthermore, telomere length and telomerase activity, which are also crucial for unlimited self-renewal and genomic stability of mESCs, do not differ in mESCs cultured under OSM or LIF. The transcriptome of mESCs cultured in OSM overall is very similar to that of LIF, and OSM activates Stat3 signaling pathway, like LIF. Additionally, OSM upregulates pentose and glucuronate interconversion, ascorbate and aldarate metabolism, and steroid and retinol metabolic pathways. Although the significance of these pathways remains to be determined, our data shows that OSM can maintain naïve pluripotent stem cells in the absence of LIF.

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Salvianolic Acid B Maintained Stem Cell Pluripotency and Increased Proliferation Rate by Activating Jak2–Stat3 Combined with EGFR–Erk1/2 Pathways

Cited by 10 publications

References 30 publications

The novel application of cordycepin in maintaining stem cell pluripotency and increasing iPS cell generation efficiency

The novel application of cordycepin in maintaining stem cell pluripotency and increasing iPS cell generation efficiency

Salvianolic acidï¿½B recovers cognitive deficits and angiogenesis in a cerebral small vessel disease rat model via the STAT3/VEGF signaling pathway

Oncostatin M Maintains Naïve Pluripotency of mESCs by Tetraploid Embryo Complementation (TEC) Assay

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