Salt tolerance of two perennial grass Brachypodium sylvaticum accessions

Sade, Nir; Wilhelmi, María del Mar Rubio; Ke, Xiaojuan; Brotman, Yariv; Wright, Matthew; Khan, Imran; Souza, Wagner Rodrigo de; Bassil, Elias; Tobias, Christian M.; Thilmony, Roger; Vogel, John P.; Blumwald, Eduardo

doi:10.1007/s11103-017-0696-3

Cited by 4 publications

(2 citation statements)

References 51 publications

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“…Compared with the control group, the Na + /K + of B. distachyon under 200 mM NaCl stress increased 3.29 times, from 0.577 to 1.895. This is consistent with the research results of Sade et al in Brachypodium sylvaticum [48]. In addition, a number of studies have shown that Clcarriers located in plasma membrane are upregulated to preserve the charge equilibrium in the cytoplasm during salt stress [5,49,50].…”

Section: Discussionsupporting

confidence: 92%

Screening of Salt Stress Responsive Genes in Brachypodium distachyon (L.) Beauv. by Transcriptome Analysis

Guo

Wang

Liu

et al. 2020

Plants

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As one of the most common abiotic stresses, salt stress seriously impairs crop yield. Brachypodium distachyon (L.) Beauv. is a model species for studying wheat and other grasses. In the present investigation, the physiological responses of B. distachyon treated with different concentrations of NaCl for 24 h were measured. Therefore, the control and the seedlings of B. distachyon treated with 200 mM NaCl for 24 h were selected for transcriptome analysis. Transcriptome differential analysis showed that a total of 4116 differentially expressed genes (DEGs) were recognized, including 3120 upregulated and 996 downregulated ones. GO enrichment assay indicated that some subsets of genes related to the active oxygen scavenging system, osmoregulatory substance metabolism, and abscisic-acid (ABA)-induced stomatal closure were significantly upregulated under salt stress. The MapMan analysis revealed that the upregulated genes were dramatically enriched in wax metabolic pathways. The expressions of transcription factor (TF) family members such as MYB, bHLH, and AP2/ERF were increased under salt stress, regulating the response of plants to salt stress. Collectively, these findings provided valuable insights into the mechanisms underlying the responses of grass crops to salt stress.

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Section: Discussionsupporting

confidence: 92%

Screening of Salt Stress Responsive Genes in Brachypodium distachyon (L.) Beauv. by Transcriptome Analysis

Guo

Wang

Liu

et al. 2020

Plants

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“…on day 10 according to the PET imaging timeline), the root and shoot of each seedling were separated. For Na + content, roots and shoots were oven dried for 3 days at 70 °C and then the tissue was scaled, grinded, digested with nitric acid and hydrogen peroxide and finally analyzed using Inductively Coupled Plasma Atomic Emission Spectrometry (ICPAES) 33 . The amount of Na + was determined as a percentage of dry weight.…”

Section: Methodsmentioning

confidence: 99%

Imaging Salt Uptake Dynamics in Plants Using PET

Ariño‐Estrada

Mitchell

Saha³

et al. 2019

Sci Rep

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Soil salinity is a global environmental challenge for crop production. Understanding the uptake and transport properties of salt in plants is crucial to evaluate their potential for growth in high salinity soils and as a basis for engineering varieties with increased salt tolerance. Positron emission tomography (PET), traditionally used in medical and animal imaging applications for assessing and quantifying the dynamic bio-distribution of molecular species, has the potential to provide useful measurements of salt transport dynamics in an intact plant. Here we report on the feasibility of studying the dynamic transport of 22Na in millet using PET. Twenty-four green foxtail (Setaria viridis L. Beauv.) plants, 12 of each of two different accessions, were incubated in a growth solution containing 22Na+ ions and imaged at 5 time points over a 2-week period using a high-resolution small animal PET scanner. The reconstructed PET images showed clear evidence of sodium transport throughout the whole plant over time. Quantitative region-of-interest analysis of the PET data confirmed a strong correlation between total 22Na activity in the plants and time. Our results showed consistent salt transport dynamics within plants of the same variety and important differences between the accessions. These differences were corroborated by independent measurement of Na+ content and expression of the NHX transcript, a gene implicated in sodium transport. Our results demonstrate that PET can be used to quantitatively evaluate the transport of sodium in plants over time and, potentially, to discern differing salt-tolerance properties between plant varieties. In this paper, we also address the practical radiation safety aspects of working with 22Na in the context of plant imaging and describe a robust pipeline for handling and incubating plants. We conclude that PET is a promising and practical candidate technology to complement more traditional salt analysis methods and provide insights into systems-level salt transport mechanisms in intact plants.

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Correlation-based network analysis combined with machine learning techniques highlight the role of the GABA shunt in Brachypodium sylvaticum freezing tolerance

Toubiana

Sade

Liu

et al. 2020

Sci Rep

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Perennial grasses will account for approximately 16 billion gallons of renewable fuels by the year 2022, contributing significantly to carbon and nitrogen sequestration. However, perennial grasses productivity can be limited by severe freezing conditions in some geographical areas, although these risks could decrease with the advance of climate warming, the possibility of unpredictable early cold events cannot be discarded. We conducted a study on the model perennial grass Brachypodium sylvaticum to investigate the molecular mechanisms that contribute to cold and freezing adaption. The study was performed on two different B. sylvaticum accessions, Ain1 and Osl1, typical to warm and cold climates, respectively. Both accessions were grown under controlled conditions with subsequent cold acclimation followed by freezing stress. For each treatment a set of morphological parameters, transcription, metabolite, and lipid profiles were measured. State-of-the-art algorithms were employed to analyze cross-component relationships. Phenotypic analysis revealed higher adaption of Osl1 to freezing stress. Our analysis highlighted the differential regulation of the TCA cycle and the GABA shunt between Ain1 and Osl1. Osl1 adapted to freezing stress by repressing the GABA shunt activity, avoiding the detrimental reduction in fatty acid biosynthesis and the concomitant detrimental effects on membrane integrity.The increase in energy demand coupled with the environmental impact of fossil fuels has led to the expansion of programs using perennial species as an alternative source of energy. In this context, perennial grasses, traditionally used as forage and turf, have become the preferred candidates for biomass cropping systems attributed to their ability to grow under low input conditions and high interception of sunlight 1 . Predictions showed that perennial grasses will account for approximately 16 billion gallons of renewable fuels by the year 2022 2,3 . Perennial grasses have shown to support carbon (C) and nitrogen (N) sequestration in the soil 4-6 , contributing to mitigate climate change and environmental degradation 7-9 . Brachypodium sylvaticum is a perennial grass with a simple genome, self-fertility, a short life cycle, and low growth requirements. Its recently released genome, expression gene atlas 10 and well established genetic transformation have made it an efficient model to study the genetic, molecular, and physiological components of perennial grasses 11,12 .Unfavorable winter conditions, including freezing, limit the geographical range of many important crops and reduce their productivity due to growth repression 13,14 . Low temperatures combined with high light intensity provoke the inhibition of the photosystem II and ROS production leading to oxidative stress 15 . In addition, ice formation in the apoplast induces both, cell membrane integrity stress and osmotic stress 16 . The plant's response to low temperatures involves extensive reprograming of gene expression via transcription factors 17 , resulting in co...

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Salt tolerance of two perennial grass Brachypodium sylvaticum accessions

Cited by 4 publications

References 51 publications

Screening of Salt Stress Responsive Genes in Brachypodium distachyon (L.) Beauv. by Transcriptome Analysis

Screening of Salt Stress Responsive Genes in Brachypodium distachyon (L.) Beauv. by Transcriptome Analysis

Imaging Salt Uptake Dynamics in Plants Using PET

Correlation-based network analysis combined with machine learning techniques highlight the role of the GABA shunt in Brachypodium sylvaticum freezing tolerance

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