Screening of Salt Stress Responsive Genes in Brachypodium distachyon (L.) Beauv. by Transcriptome Analysis

Abstract

As one of the most common abiotic stresses, salt stress seriously impairs crop yield. Brachypodium distachyon (L.) Beauv. is a model species for studying wheat and other grasses. In the present investigation, the physiological responses of B. distachyon treated with different concentrations of NaCl for 24 h were measured. Therefore, the control and the seedlings of B. distachyon treated with 200 mM NaCl for 24 h were selected for transcriptome analysis. Transcriptome differential analysis showed that a total of 4116 differentially expressed genes (DEGs) were recognized, including 3120 upregulated and 996 downregulated ones. GO enrichment assay indicated that some subsets of genes related to the active oxygen scavenging system, osmoregulatory substance metabolism, and abscisic-acid (ABA)-induced stomatal closure were significantly upregulated under salt stress. The MapMan analysis revealed that the upregulated genes were dramatically enriched in wax metabolic pathways. The expressions of transcription factor (TF) family members such as MYB, bHLH, and AP2/ERF were increased under salt stress, regulating the response of plants to salt stress. Collectively, these findings provided valuable insights into the mechanisms underlying the responses of grass crops to salt stress.

Changes in the Cell Wall Proteome of Leaves in Response to High Temperature Stress in Brachypodium distachyon

“…For example, an overexpression of A. thaliana LTL1 leads to an increased salt tolerance [66]. Notably, the expression of GDSL-lipases/acylhydrolases varies significantly in response to salt stress as was shown for B. distachyon roots [56]. In the experiment presented here, four GDSL-lipases/acylhydrolases were under-accumulated at 40 • C, suggesting a decrease in hydrolysis and lipid synthesis in response to heat stress.…”

“…In our analysis, one over-accumulated and one under-accumulated dirigent protein were identified. Numerous genes encoding the dirigent proteins were found to be up-regulated in response to salt stress in the roots of B. distachyon, among which Bradi1g20950 encoding a CWP that was found among the over-accumulated CWPs in this experiment [56]. In Medicago sativa, a gene encoding a dirigent protein was up-regulated due to heat stress [57].…”

Understanding the physiological and molecular mechanism of salinity stress tolerance in plants

“…Under salinity stress, auxin enhances the expression of a number of candidate genes that are involved in auxin biosynthesis and signaling pathways, suggesting the involvement of auxin in salinity stress tolerance. For example, the expressions of PIN2, Auxin influx carrier, GH3, SAUR, and auxin response factor genes are downregulated under salt stress (Guo et al, 2020;Ribba et al, 2020). IAA plays an essential role in coping with stress by producing various DEGs; transcription of genes like gp1 and indole-3-acetic acid-amido synthetase helps vegetative cell elongation.…”

Study on the Effect of the Copper Tailing Substrate with Different Treatments on the Growth of Tall Fescue (Festuca arundinacea Schreb)

“…Then, the dry weight was measured. Beyond that, the ethanol extraction method was adopted to measure the content of chlorophyll a, chlorophyll b in this study (Li et al 2022), while Catalase activity was determined using UV spectrophotometry (Guo et al 2020), and the Cu 2+ content was determined using inductively coupled plasma atomic emission spectroscopy (Tan and Yu et al 2019). Furthermore, the pH value of the substrate was determined by the water extraction potential method (the ratio of water to soil was 2.5:1) (Zheng et al 2021).…”