Salt tolerance of a Sinorhizobium meliloti strain isolated from dry lands: growth capacity and protein profile changes

Mrabet, Moncef; Zribi, Kais; Mhadhbi, Haythem; Djébali, Naceur; Mhamdi, Ridha; Aouani, Mohamed Elarbi; Nakamura, Kouji

doi:10.1007/s13213-010-0153-x

Cited by 7 publications

(9 citation statements)

References 38 publications

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“…In the present study, accumulations of four proteins related to protein metabolism were repressed by salt stress, including zinc protease, elongation factor Ts, and the 30S ribosomal protein S6 (spots 5–8; Table 2 ). Consistent with our findings, metabolism-related proteins are regulated by salt stress at the protein or transcriptional level [ 42 , 45 , 46 ]. Most of the salt-repressed genes encoding proteins, such as ribosomal proteins and elongation factors, in S. meliloti are involved in metabolism, suggesting that protein synthesis is probably inhibited under salt stress [ 45 ].…”

Section: Discussionsupporting

confidence: 91%

“…ABC transporter proteins play important roles in the transportation of nutrients and small molecules are involved in cellular osmoprotection and contribute to bacterial adaptation to hyperosmolarity. ABC transporters are regulated by salt stress in many rhizobial strains, such as S. meliloti , R. etli , M. ciceri , and R. tropici [ 40 , 41 , 42 , 43 ]. For example, salt stress induces the accumulation of a putative oligopeptide ABC transporter ATP-binding protein in the halotolerant strain A5.…”

Section: Discussionmentioning

confidence: 99%

“…For example, salt stress induces the accumulation of a putative oligopeptide ABC transporter ATP-binding protein in the halotolerant strain A5. The putative oligopeptide ABC transporter ATP-binding protein may be involved in the transportation of small molecules (e.g., amino acids, amines, and peptides), and it plays a key role in the modulation of solute diffusion under osmotic stress in strain A5 [ 42 ]. Therefore, the regulation of ABC transporter systems combined with the de novo biosynthesis of an osmoprotectant might be a potential adaptive strategy of the salt-tolerant strain RJS9-2 in response to high salinity.…”

Section: Discussionmentioning

confidence: 99%

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High Salt Tolerance of a Bradyrhizobium Strain and Its Promotion of the Growth of Stylosanthes guianensis

Dong

Zhang

Hengfu

et al. 2017

IJMS

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Salinity is a serious limiting factor for the growth of rhizobia. Some rhizobia are tolerant to salt stress and promote plant growth, but the mechanisms underlying these effects are poorly characterized. The growth responses and osmoprotectants in four Bradyrhizobium strains were examined under salt stress in this study. Two-dimensional electrophoresis (2-DE) and mass spectrometry were conducted to investigate protein profiles in rhizobia exposed to salt stress. Subsequently, salt tolerance in stylo (Stylosanthes guianensis) inoculated with rhizobia was further detected in hydroponics. Results showed that the Bradyrhizobium strain RJS9-2 exhibited higher salt tolerance than the other three Bradyrhizobium strains. RJS9-2 was able to grow at 0.35 M NaCl treatment, while the other three Bradyrhizobium strains did not grow at 0.1 M NaCl treatment. Salt stress induced IAA production, and accumulation of proline, betaine, ectoine, and trehalose was observed in RJS9-2 but not in PN13-1. Proteomics analysis identified 14 proteins regulated by salt stress in RJS9-2 that were mainly related to the ABC transporter, stress response, and protein metabolism. Furthermore, under saline conditions, the nodule number, plant dry weight, and N concentration in stylo plants inoculated with RJS9-2 were higher than those in plants inoculated with PN13-1. These results suggest that the tolerance of RJS9-2 to salt stress may be achieved by the coordination of indole-3-acetic acid (IAA) production, osmoprotectant accumulation, and protein expression, thus promoting stylo growth.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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High Salt Tolerance of a Bradyrhizobium Strain and Its Promotion of the Growth of Stylosanthes guianensis

Dong

Zhang

Hengfu

et al. 2017

IJMS

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“…Mutants of L. monocytogenes lacking Tig showed susceptibility to ethanol stress [39] and activation of both trigger factor and cyclophilin are essential for growth under starvation conditions [38] and during cold-shock response [35] in Bacillus subtilis as well as the survival of a soil bacterium Sinorhizobium meliloti under hyperosmotic conditions induced by NaCl [40,41]. …”

Section: Resultsmentioning

confidence: 99%

Characterisation of the Transcriptomes of Genetically Diverse Listeria monocytogenes Exposed to Hyperosmotic and Low Temperature Conditions Reveal Global Stress-Adaptation Mechanisms

2013

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The ability of Listeria monocytogenes to adapt to various food and food- processing environments has been attributed to its robustness, persistence and prevalence in the food supply chain. To improve the present understanding of molecular mechanisms involved in hyperosmotic and low-temperature stress adaptation of L. monocytogenes, we undertook transcriptomics analysis on three strains adapted to sub-lethal levels of these stress stimuli and assessed functional gene response. Adaptation to hyperosmotic and cold-temperature stress has revealed many parallels in terms of gene expression profiles in strains possessing different levels of stress tolerance. Gene sets associated with ribosomes and translation, transcription, cell division as well as fatty acid biosynthesis and peptide transport showed activation in cells adapted to either cold or hyperosmotic stress. Repression of genes associated with carbohydrate metabolism and transport as well as flagella was evident in stressed cells, likely linked to activation of CodY regulon and consequential cellular energy conservation.

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“…In the second step we investigated mechanisms involved in stress response (tolerance/sensitivity) of free living bacteria with the aim to identify pertinent marker of stress tolerance. For this purpose a proteomic approach was previously performed to analyze differential response of Sinorhizobium strains to salt stress and to identify stress‐response related peptides using 2D‐PAGE . In the present study, we investigated membrane lipids and antioxidant enzymes’ modulation to identify biochemical markers of stress response within rhizobial strain already known to have positive symbiosis response to osmotic stresses.…”

Section: Discussionmentioning

confidence: 99%

Growth capacity and biochemical mechanisms involved in rhizobia tolerance to salinity and water deficit

Nouairi

Hammouda

Mhamdi

et al. 2014

J. Basic Microbiol.

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The aim of the present study was to evaluate abiotic stress tolerance of rhizobial strains belonging to Mesorhizobium, Sinorhizobium, and Rhizobium genera, as well as to investigate specie specific stress response mechanisms. Effect of NaCl and PEG on growth capacity, protein, lipid peroxydation (MDA), membrane fatty acid composition and antioxidant enzymes were investigated. Growth capacity and viability of overall rhizobia strains decreased proportionally to the increase of NaCl and PEG levels in the medium. Sinorhizobium strains appeared the most tolerant, where 4H41strain was able to grow at 800 mM NaCl and 40% PEG. On the other hand, growth of R. gallicum and M. mediterraneum was inhibited by 200 mM NaCl. The content of MDA was unchanged in Sinorhizobium strains under both stresses. For Mesorhizobium, only PEG treatment increased the content of MDA. Amount of the C19:0 cyclo fatty-acid was increased in both Sinorhizobium and Mesorhizobium tolerant strains. NaCl stress increased Superoxide dismutase (SOD) activity of overall species; especially the most tolerant strain 4H41. Both treatments increased catalase (CAT) activity in 4H41, TII7, and 835 strains. Obtained results suggest that major response of tolerant Sinorhizobium and Mesorhizobium strains to NaCl and PEG stresses is a preferential accumulation of the C19:0 cyclo fatty acid within bacterial membrane as mechanism to reduce fluidity and maintain integrity. Cell integrity and functioning is also assured by maintaining and/or increasing activity of SOD and CAT antioxidant enzymes for tolerant strains to omit structural and functional damages related to reactive oxygen species overproduced under stressful conditions.

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Salt tolerance of a Sinorhizobium meliloti strain isolated from dry lands: growth capacity and protein profile changes

Cited by 7 publications

References 38 publications

High Salt Tolerance of a Bradyrhizobium Strain and Its Promotion of the Growth of Stylosanthes guianensis

High Salt Tolerance of a Bradyrhizobium Strain and Its Promotion of the Growth of Stylosanthes guianensis

Characterisation of the Transcriptomes of Genetically Diverse Listeria monocytogenes Exposed to Hyperosmotic and Low Temperature Conditions Reveal Global Stress-Adaptation Mechanisms

Growth capacity and biochemical mechanisms involved in rhizobia tolerance to salinity and water deficit

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