Characterisation of the Transcriptomes of Genetically Diverse Listeria monocytogenes Exposed to Hyperosmotic and Low Temperature Conditions Reveal Global Stress-Adaptation Mechanisms

Durack, Juliana; Ross, Tom; Bowman, John P.

doi:10.1371/journal.pone.0073603

Cited by 60 publications

(54 citation statements)

References 95 publications

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“…Furthermore, L . monocytogenes CSPs appear to be only induced during the early stages following cold stress [11, 12, 143]. In the present study, cspB was downregulated at all growth phases, reaching a maximum 228-fold decrease at G5, while cspD and cspL were upregulated at G1–G2 and then exhibited no change or decreased expression at the later growth phases (Table 6).…”

Section: Resultssupporting

confidence: 47%

“…Genes co-regulated by RpoD and PrfA include prfA , plcAB , hly , mpl , inlC , and actA , all of which are associated with virulence [109]. Other studies have also reported lower levels of PrfA-regulated virulence genes in cold-adapted exponential and stationary-phase cells [11, 12, 110]. In the present study, the DE pattern of prfA fit that of cluster 17 (Fig 5), with 2-fold and 40-fold increased expression at G3 and G5, respectively (Table 6).…”

Section: Resultsmentioning

confidence: 99%

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Strand specific RNA-sequencing and membrane lipid profiling reveals growth phase-dependent cold stress response mechanisms in Listeria monocytogenes

et al. 2017

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The human pathogen Listeria monocytogenes continues to pose a challenge in the food industry, where it is known to contaminate ready-to-eat foods and grow during refrigerated storage. Increased knowledge of the cold-stress response of this pathogen will enhance the ability to control it in the food-supply-chain. This study utilized strand-specific RNA sequencing and whole cell fatty acid (FA) profiling to characterize the bacterium’s cold stress response. RNA and FAs were extracted from a cold-tolerant strain at five time points between early lag phase and late stationary-phase, both at 4°C and 20°C. Overall, more genes (1.3×) were suppressed than induced at 4°C. Late stationary-phase cells exhibited the greatest number (n = 1,431) and magnitude (>1,000-fold) of differentially expressed genes (>2-fold, p<0.05) in response to cold. A core set of 22 genes was upregulated at all growth phases, including nine genes required for branched-chain fatty acid (BCFA) synthesis, the osmolyte transporter genes opuCBCD, and the internalin A and D genes. Genes suppressed at 4°C were largely associated with cobalamin (B12) biosynthesis or the production/export of cell wall components. Antisense transcription accounted for up to 1.6% of total mapped reads with higher levels (2.5×) observed at 4°C than 20°C. The greatest number of upregulated antisense transcripts at 4°C occurred in early lag phase, however, at both temperatures, antisense expression levels were highest in late stationary-phase cells. Cold-induced FA membrane changes included a 15% increase in the proportion of BCFAs and a 15% transient increase in unsaturated FAs between lag and exponential phase. These increases probably reduced the membrane phase transition temperature until optimal levels of BCFAs could be produced. Collectively, this research provides new information regarding cold-induced membrane composition changes in L. monocytogenes, the growth-phase dependency of its cold-stress regulon, and the active roles of antisense transcripts in regulating its cold stress response.

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Section: Resultssupporting

confidence: 47%

Section: Resultsmentioning

confidence: 99%

Strand specific RNA-sequencing and membrane lipid profiling reveals growth phase-dependent cold stress response mechanisms in Listeria monocytogenes

et al. 2017

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“…In Sphingopyxis alaskensis, a high abundance of proteins involved in cell wall biogenesis was described at 10 °C including a membrane structural lipoprotein OmpA which acts in the optimization of the structure and function of the membrane [25]. Recently, a transcriptomic analysis of Listeria monocytogenes cultivated under low temperatures and osmotic stress revealed the upregulation of genes associated with the biosynthesis of peptidoglycan and fatty acid molecules [28]. …”

Section: Mechanisms Of Bacterial Adaptation To Coldmentioning

confidence: 99%

A Proteomic Perspective on the Bacterial Adaptation to Cold: Integrating OMICs Data of the Psychrotrophic Bacterium Exiguobacterium antarcticum B7

et al. 2017

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Since the publication of one of the first studies using 2D gel electrophoresis by Patrick H. O’Farrell in 1975, several other studies have used that method to evaluate cellular responses to different physicochemical variations. In environmental microbiology, bacterial adaptation to cold environments is a “hot topic” because of its application in biotechnological processes. As in other fields, gel-based and gel-free proteomic methods have been used to determine the molecular mechanisms of adaptation to cold of several psychrotrophic and psychrophilic bacterial species. In this review, we aim to describe and discuss these main molecular mechanisms of cold adaptation, referencing proteomic studies that have made significant contributions to our current knowledge in the area. Furthermore, we use Exiguobacterium antarcticum B7 as a model organism to present the importance of integrating genomic, transcriptomic, and proteomic data. This species has been isolated in Antarctica and previously studied at all three omic levels. The integration of these data permitted more robust conclusions about the mechanisms of bacterial adaptation to cold.

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“…3), leading to the accumulation of pyruvate. On the contrary, single stresses, such as cold, acid or salt, increased the levels of the pleiotropic repressor CodY in the cells, presumably to repress energy-consuming processes for cellular energy conservation (Bowman et al 2012;Durack et al 2013).…”

Section: Transcriptional Regulationmentioning

confidence: 99%

Proteomics analysis of Listeria monocytogenes ATCC 19115 in response to simultaneous triple stresses

Deng

Chen

et al. 2015

Arch Microbiol

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Listeria monocytogenes can cause listeriosis in humans through consumption of contaminated food. L. monocytogenes can adapt and grow in a vast array of physiochemical stresses in the food production environment. In this study, we performed a proteomics strategy in order to investigate how L. monocytogenes survives with a simultaneous exposure to low pH, high salinity and low temperature. The results showed that the adaptation processes mainly affected the biochemical pathways related to protein synthesis, oxidative stress, cell wall and nucleotide metabolism. Interestingly, enzymes involved in the carbohydrate metabolism of energy, such as glycolysis and pentose phosphate pathway, were derepressed due to the down-regulation of CodY, a global transcriptional repressor. The down-regulation of CodY, together with the up-regulation of carbohydrate metabolism enzymes, likely leads to the accumulation of pyruvate and further to the activation of fatty acid synthesis pathway. Proteomics profiling offered a better understanding of the physiological responses of this pathogen to adapt to harsh environment and would hopefully contribute to improving the food-processing and storage methods.

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Characterisation of the Transcriptomes of Genetically Diverse Listeria monocytogenes Exposed to Hyperosmotic and Low Temperature Conditions Reveal Global Stress-Adaptation Mechanisms

Cited by 60 publications

References 95 publications

Strand specific RNA-sequencing and membrane lipid profiling reveals growth phase-dependent cold stress response mechanisms in Listeria monocytogenes

Strand specific RNA-sequencing and membrane lipid profiling reveals growth phase-dependent cold stress response mechanisms in Listeria monocytogenes

A Proteomic Perspective on the Bacterial Adaptation to Cold: Integrating OMICs Data of the Psychrotrophic Bacterium Exiguobacterium antarcticum B7

Proteomics analysis of Listeria monocytogenes ATCC 19115 in response to simultaneous triple stresses

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