Salt stress inhibits photosystems II and I in cyanobacteria

Allakhverdiev, Suleyman I.; Murata, Norio

doi:10.1007/s11120-008-9334-x

Cited by 156 publications

(108 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The second step involved the transfer of aminoacyl of the t-RNA. Phycobillisomes are the major light harvesting complexes of cyanobacteria under nitrogen fixing condition and under salt stress conditions; major component of the phycobilisomes is strongly expressed [36,59]. The above findings are in agreement with our interpretations.…”

supporting

confidence: 89%

Proteomic Responses of the Cyanobacterium Nostoc Muscorum under Salt and Osmotic Stresses

Gupta¹,

Bhardwaj²,

Gothalwal³

et al. 2017

JAMB

View full text Add to dashboard Cite

Abstract. In this paper, we examined the effect of salt stress (NaCl) and osmotic stress (sucrose) on proteomic level in the diazotrophic cyanobacterium Nostoc muscorum. The aim of this study is to compare proteins appeared in control vs. salt treated, control vs. sucrose treated and salt treated vs. sucrose treated cultures. In the salt treated cultures about 37 proteins were expressed differentially out of these only 5 proteins have shown fold regulation of 1.5 or more. About 141 proteins were found to express independently in control and about 554 proteins were express independently in salt treated culture. When we compared proteins in control and sucrose treated cells, it was reported that about 37 protein spots were express differentially, out of these only 7 proteins have fold regulation 1.5 or more. The independently expressed proteins appeared on gel are 141 and 186 respectively. Similarly, when we compared proteins appeared in salt and sucrose treated cells, it was reported that about 54 proteins were express differentially, out of these 10 proteins have fold regulation 1.5 or more. About 537 protein spots were independently present in salt treated cells and about 186 proteins were independently present in sucrose treated cells. In addition, the differentially expressed proteins and their identification with their functional group have also been discussed. Key words:Nostoc muscorum, osmotic stress, proteomic, salt stress IntroductionCyanobacteria are Gram negative eubacteria, their evolutionary history dated back to 2.7 billion years ago [1]. The origin of cyanobacteria and the evolution of oxygenic photosynthesis have been considered as the most important event in the evolution of aerobic atmosphere. Cyanobacteria are known to be found in almost all the ecological niches with diverse environmental conditions. The native cyanobacterial species present in such habitats confronted with cation toxicity and water loss. The microorganisms, including cyanobacteria that grow and multiply in such stressful habitats have ability to change their morphological and physiological parameters to cope up with such stressful conditions [2]. The ionic component of the stress factor is usually overcome by the efflux mechanism driven by Na + /H + antiporter activity or by the Mrp system [3,4,2]. On the other hand the osmotic component of the stress factor is overcome by the synthesis/accumulation of low molecular weight organic compounds collectively known as compatible solutes [5,6]. The nature and the biosynthesis of compatible solutes depend upon the habitat in which cyanobacteria grow. The fresh water cyanobacterial strains are known to synthesized sucrose, trehalose and proline as an osmotic balancer [7,2,8]. Glucosyl-glycerol is a major compatible solute synthesized by moderately halotolerant strains [9,10]. On the other hand hyper saline strains produce glycine-betaine or glutamate-betaine as compatible solutes [11,12].The modern molecular biology techniques such as genomics and proteomics have provided valuable dat...

show abstract

supporting

confidence: 89%

Proteomic Responses of the Cyanobacterium Nostoc Muscorum under Salt and Osmotic Stresses

Gupta¹,

Bhardwaj²,

Gothalwal³

et al. 2017

JAMB

View full text Add to dashboard Cite

show abstract

“…Preparation of growth medium with added calcium N t = N 0 ·e rn·t [1] Where N t is the number of cells in time t; N 0 is the initial number of cells; and r n is the growth rate (33).…”

Section: Methods Strain Maintenancementioning

confidence: 99%

The inhibitory effect of calcium on Cylindrospermopsis raciborskii (cyanobacteria) metabolism

Carneiro¹,

Alípio²,

Bisch³

et al. 2011

Braz. J. Microbiol.

View full text Add to dashboard Cite

Cylindrospermopsis raciborskii (Woloszynska) Seenaya & Subba Raju is a freshwater cyanobacterium of worldwide distribution. In the North-eastern region of Brazil many eutrophic water reservoirs are characterized by the dominance of C. raciborskii, with recurrent occurrence of blooms. These water bodies have high conductivity due to a high ionic concentration, and are defined as hard (with high values of CaCO 3 ). In this study, we investigated the long-term effect (12 days) of high calcium concentration (8 mM Ca 2+ ) on C. raciborskii (T3 strain) growth, morphology, toxin content, and metabolism. Changes in protein expression profiles were investigated by proteomic analysis using 2D gel electrophoresis and mass spectrometry. A continued exposure to calcium had a pronounced effect on C. raciborskii (T3): it limited growth, decreased thricome length, increased chlorophyll-a content, altered toxin profile (although did not affect PST content, saxitoxin + neosaxitoxin), and inhibited the expression of proteins related to primary metabolism.

show abstract

“…As a further indicator of the potential importance of salinity in defining microbial mat and microbialite communities, salt stress has been shown to inhibit the photosynthetic capability of cyanobacteria (Allakhverdiev and Murata, 2008;Hagemann, 2011), which may further explain these metagenomic differences observed. Furthermore, it has been suggested that Pavilion Lake microbialite heterotrophs contribute to the cementation process of thrombolites by subsequent in-filling of microstructures (Omelon et al, 2013), a process that could potentially be occurring in Shark Bay systems.…”

Section: Stress Responsementioning

confidence: 99%

Unravelling core microbial metabolisms in the hypersaline microbial mats of Shark Bay using high-throughput metagenomics

et al. 2015

View full text Add to dashboard Cite

Modern microbial mats are potential analogues of some of Earth's earliest ecosystems. Excellent examples can be found in Shark Bay, Australia, with mats of various morphologies. To further our understanding of the functional genetic potential of these complex microbial ecosystems, we conducted for the first time shotgun metagenomic analyses. We assembled metagenomic nextgeneration sequencing data to classify the taxonomic and metabolic potential across diverse morphologies of marine mats in Shark Bay. The microbial community across taxonomic classifications using protein-coding and small subunit rRNA genes directly extracted from the metagenomes suggests that three phyla Proteobacteria, Cyanobacteria and Bacteriodetes dominate all marine mats. However, the microbial community structure between Shark Bay and Highbourne Cay (Bahamas) marine systems appears to be distinct from each other. The metabolic potential (based on SEED subsystem classifications) of the Shark Bay and Highbourne Cay microbial communities were also distinct. Shark Bay metagenomes have a metabolic pathway profile consisting of both heterotrophic and photosynthetic pathways, whereas Highbourne Cay appears to be dominated almost exclusively by photosynthetic pathways. Alternative non-rubisco-based carbon metabolism including reductive TCA cycle and 3-hydroxypropionate/4-hydroxybutyrate pathways is highly represented in Shark Bay metagenomes while not represented in Highbourne Cay microbial mats or any other mat forming ecosystems investigated to date. Potentially novel aspects of nitrogen cycling were also observed, as well as putative heavy metal cycling (arsenic, mercury, copper and cadmium). Finally, archaea are highly represented in Shark Bay and may have critical roles in overall ecosystem function in these modern microbial mats.

show abstract

Salt stress inhibits photosystems II and I in cyanobacteria

Cited by 156 publications

References 69 publications

Proteomic Responses of the Cyanobacterium Nostoc Muscorum under Salt and Osmotic Stresses

Proteomic Responses of the Cyanobacterium Nostoc Muscorum under Salt and Osmotic Stresses

The inhibitory effect of calcium on Cylindrospermopsis raciborskii (cyanobacteria) metabolism

Unravelling core microbial metabolisms in the hypersaline microbial mats of Shark Bay using high-throughput metagenomics

Contact Info

Product

Resources

About