Salt dependent resistance against chemical denaturation of alkaline protease from a newly isolated haloalkaliphilic Bacillus sp.

Dodia, M. S.; Bhimani, H. G.; Rawal, C. M.; Joshi, Rupal H.; Singh, Satya P.

doi:10.1016/j.biortech.2007.12.020

Cited by 58 publications

(33 citation statements)

References 17 publications

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“…Interestingly, proteases from haloalkaliphilic bacteria and salt-tolerant alkaliphilic actinomycetes were highly resistant to chemical denaturation by urea [35,36,40].…”

Section: Chemical Denaturation Of Amylase Using Urea and Guanidine-hclmentioning

confidence: 99%

Single step purification and characterization of a thermostable and calcium independent α-amylase from Bacillus amyloliquifaciens TSWK1-1 isolated from Tulsi Shyam hot spring reservoir, Gujarat (India)

Kikani

Singh

2011

International Journal of Biological Macromolecules

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“…Interestingly, proteases from haloalkaliphilic bacteria and salt-tolerant alkaliphilic actinomycetes were highly resistant to chemical denaturation by urea [35,36,40].…”

Section: Chemical Denaturation Of Amylase Using Urea and Guanidine-hclmentioning

confidence: 99%

Single step purification and characterization of a thermostable and calcium independent α-amylase from Bacillus amyloliquifaciens TSWK1-1 isolated from Tulsi Shyam hot spring reservoir, Gujarat (India)

Kikani

Singh

2011

International Journal of Biological Macromolecules

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“…As an extension of our on-going research on haloalkaliphilic bacteria form Coastal Gujarat [33][34][35], the present study aims at the optimization, assessment and comparison of the extraction methods for total environmental DNA from two different habitats; saline soil near salt pan of Okha Madhi, Gujarat Coast and Sambhar Soda Lake, Rajasthan, both in India. The protocols described in the present report signify the extraction of DNA from different saline habitats without any prior treatment.…”

Section: Introductionmentioning

confidence: 99%

Comparative studies on the extraction of metagenomic DNA from the saline habitats of Coastal Gujarat and Sambhar Lake, Rajasthan (India) in prospect of molecular diversity and search for novel biocatalysts

Siddhapura

Vanparia

Purohit

et al. 2010

International Journal of Biological Macromolecules

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“…Dodia et al have reported salt dependent resistance of alkaline protease to chemical denaturants from a newly isolated haloalkaliphilic Bacillus sp. [16].…”

Section: Discussionmentioning

confidence: 95%

Purification, characterization and thermodynamics of antifungal protease from Streptomyces sp. A6

Singh

Chhatpar

2011

J. Basic Microbiol.

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A 20 kDa antifungal serine protease from Streptomyces sp. A6 was purified to 34.56 folds by gel permeation chromatography. The enzyme exhibited highest activity at neutral to near alka- line pH 7-9 and 55 °C. Neutral surfactant triton X-100 enhanced the activity by 4.12 fold. The protease activity also increased (109.9-119%) with increasing concentration of urea (2-8 mole/l). The enzyme was identified as serine protease with 67% similarity to SFase 2 of Streptomyces fradiae by MALDI-LC-MS/MS analysis. Determination of kinetic constants k(m) , V(max) , k(cat) and k(cat) /k(m) suggested higher affinity of enzyme for N-Suc-Ala-Ala-Val-Ala-p NA (synthetic substrate for chymotrypsin activity). The enzyme was highly stable at temperature prevailing under field conditions (40 °C) as apparent from K(d) and t(1/2) values, 0.0065 and 106.75 min, respectively and high ΔG* and negative ΔS * values, 87.17 KJ/mole and -126.95 J/mole, respectively. Thermal stability and increased activity of protease in presence of commonly used chemical fertilizer, urea, suggested its feasibility for agricultural applications. The present study is the first report on thermodynamic and kinetic properties of an antifungal protease from Streptomyces sp. A6. The study reflects potential of this enzyme for biocontrol of fungal plant pathogens.

show abstract

Salt dependent resistance against chemical denaturation of alkaline protease from a newly isolated haloalkaliphilic Bacillus sp.

Cited by 58 publications

References 17 publications

Single step purification and characterization of a thermostable and calcium independent α-amylase from Bacillus amyloliquifaciens TSWK1-1 isolated from Tulsi Shyam hot spring reservoir, Gujarat (India)

Single step purification and characterization of a thermostable and calcium independent α-amylase from Bacillus amyloliquifaciens TSWK1-1 isolated from Tulsi Shyam hot spring reservoir, Gujarat (India)

Comparative studies on the extraction of metagenomic DNA from the saline habitats of Coastal Gujarat and Sambhar Lake, Rajasthan (India) in prospect of molecular diversity and search for novel biocatalysts

Purification, characterization and thermodynamics of antifungal protease from Streptomyces sp. A6

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