Purification, characterization and thermodynamics of antifungal protease from <i>Streptomyces</i> sp. A6

Singh, Anil Kumar; Chhatpar, H. S.

doi:10.1002/jobm.201000310

Cited by 43 publications

(22 citation statements)

References 17 publications

(15 reference statements)

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“…Many in vitro studies support the significant involvement of crude as well as purified mycolytic enzymes (i.e. chitinase, b-1,3-glucanase and protease) either individually or in combinations inhibiting the growth of various fungal pathogens [12,23,25,28,45].…”

Section: Discussionmentioning

confidence: 99%

“…Actinomycetes (actinobacteria), particularly Streptomyces sp. are known to produce mycolytic enzymes like chitinase , protease , laminarinase (β‐1,3‐glucanase) and also combinations of these enzymes . In the present study, the potential of Streptomyces violaceusniger MTCC 3959 to synthesize mycolytic enzymes which includes chitinase, β‐1,3‐glucanase, and protease was examined.…”

Section: Introductionmentioning

confidence: 98%

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Mycolytic enzymes produced by Streptomyces violaceusniger and their role in antagonism towards wood‐rotting fungi

Nagpure

Choudhary

Gupta

2013

J. Basic Microbiol.

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Extracellular mycolytic enzymes produced under submerged fermentation by the fungal antagonist Streptomyces violaceusniger MTCC 3959 were characterized. This streptomycete produced higher amounts of extracellular chitinase and protease during late exponential phase, whereas β-1,3-glucanase production was at peak in mid-stationary phase. Cell-free culture filtrate (CCF) exhibited a broad range of antifungal activity against both white rot and brown rot fungi. The inhibitory activity was completely lost after treatment with proteinase K and heat, indicating that extracellular antifungal metabolites are heat labile and proteinaceous in nature. Optimum pH and temperature for enzyme activity were: 9.0 and 60 °C for chitinase; 6.0 and 60 °C for β-1,3-glucanase; and 9.0 and 70 °C for protease. Mycolytic enzymes were moderately thermostable, and had a wide pH stability range extending from pH 5.0 to 10.0. The zymogram analysis of CCF revealed five chitinase isoenzymes with an apparent molecular weight of 20.8, 33.3, 45.6, 67.4, and 114.8 kDa, one β-1,3-glucanase appeared as a single band of ∼131.8 kDa and four protease isoenzymes with approximate molecular weights of 22.8, 62.52, 74.64, and 120.5 kDa. S. violaceusniger MTCC 3959 produced mycolytic enzymes that can be effectively used for suppression of phytopathogenic basidiomycetes. It has the potential to be an effective biofungicide.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Mycolytic enzymes produced by Streptomyces violaceusniger and their role in antagonism towards wood‐rotting fungi

Nagpure

Choudhary

Gupta

2013

J. Basic Microbiol.

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“…), the antifungal activity of a 20‐kD protease isolated from Streptomyces sp. A6 has been the only available report on antifungal activity of a protease from a Streptomyces strain (Singh and Chhatpar ).…”

Section: Discussionmentioning

confidence: 99%

“…Three agar blocks of the same size containing C. coccodes were cut out from a profusely grown fungal plate, placed at equal distance from the central well, allowed to diffuse for 30 min at 4°C and incubated at 28°C for 5 days. Zone of inhibition was considered as a measure of antifungal activity (Singh and Chhatpar ).…”

Section: Methodsmentioning

confidence: 99%

Extracellular proteases fromStreptomyces phaeopurpureusExPro138 inhibit spore adhesion, germination and appressorium formation inColletotrichum coccodes

2013

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Aim: To study the antifungal mechanism of proteases from Streptomyces phaeopurpureus strain ExPro138 towards Colletotrichum coccodes and to evaluate its utilization as biofungicide. Methods and Results: We screened proteolytic Streptomyces strains from the yam rhizosphere with antifungal activity. Forty proteolytic Streptomyces were isolated, among which eleven isolates showed gelatinolytic activity and antagonistic activity on C. coccodes. Of the 11 isolates, protease preparation from an isolate designated ExPro138 showed antifungal activity. 16S rDNA sequence analysis of the strain showed 99% similarity with Streptomyces phaeopurepureus (EU841588.1). Zymography analysis of the ExPro138 culture filtrate revealed that the strain produced several extracellular proteases. The protease preparation inhibited spore germination, spore adhesion to polystyrene surface and appressorium formation. Microscopic study of the interaction between ExPro138 and C. coccodes revealed that ExPro138 was mycoparasitic on C. coccodes. The protease preparation also reduced anthracnose incidence on tomato fruits compared with untreated control. Conclusion: This study demonstrates possibility of utilizing antifungal proteases derived from antagonistic microbes as biofungicide. Significance and Impact of the Study: Microbial proteases having the ability to inhibit spore adhesion and appressorium formation could be used to suppress infection establishment by foliar fungal pathogens at the initial stages of the infection process.

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“…The inhibition of spore germination of the Fusarium udum was also observed by action of Streptomyces sp. A6 [6].…”

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confidence: 99%

Antifungal Activity of Bacteria of the Genus Bacillus and Streptomyces Isolated from the Soil of the Republic of Moldova

Shubina¹,

Birsa²,

Burtseva³

2018

Mikrobiol. Z.

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Aim. The article deals with the results of research, the aim of which was to determine antifungal activity of the strains Bacillus and Streptomyces genera, in relation to phytopathogenic fungi, widely distributed in Moldova. Methods. Strains were grown on classical agar media, the antifungal activity was determined by the method of agar blocks with test cultures -phytopathogenic fungi. Biological standard was the bacterium B. subtilis 26D. In the experiments on the germination of A. alternata conidia, 5 bacterial cultures grown in liquid nutrient medium No. 2. Results. It is established that all bacteria of genus Bacillus exhibit antifungal activity against test cultures, but in varying degrees. The growth of B. cinerea and F. oxysporum was least inhibited. Strain B. subtilis S4 most actively suppressed the growth of S. sclerotiorum. Strain Streptomyces sp. 10 is able to completely suppress the growth of A . alternata and B. cinerea, and the strain S. sp 9 completely suppressed the growth of S. sclerotiorum. Strains 9, 12, and 66 actively inhibited growth of F. culmorum (the radius of the zones was 16.0−20.0 mm), and strain S. sp. 185 against F. oxysporum (zones up to 28.0 mm). The strongest inhibitory effect on the germination of A. alternata conidia had strain S2. Conclusions. A comparative assessment of the antifungal activity of B. subtilis revealed a strain with the most pronounced antifungal properties. The investigated liquid cultures of bacteria of the genus Bacillus exhibited a special fungicidal effect on the germination of A. alternata conidia. Among 7 strains of streptomycetes tested, particular interest had S. sp. 10, capable to completely suppressing the growth of A. alternata and B. cinerea, and the strain S. sp. 9 -completely inhibits the growth of S. sclerotiorum, as well as the strain S. sp. 185 actively inhibited the growth of F. oxysporum and the strain S. sp. 10 − A. solani.

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Purification, characterization and thermodynamics of antifungal protease from Streptomyces sp. A6

Cited by 43 publications

References 17 publications

Mycolytic enzymes produced by Streptomyces violaceusniger and their role in antagonism towards wood‐rotting fungi

Mycolytic enzymes produced by Streptomyces violaceusniger and their role in antagonism towards wood‐rotting fungi

Extracellular proteases fromStreptomyces phaeopurpureusExPro138 inhibit spore adhesion, germination and appressorium formation inColletotrichum coccodes

Antifungal Activity of Bacteria of the Genus Bacillus and Streptomyces Isolated from the Soil of the Republic of Moldova

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