Salmonellas in Danish pigs: a comparison of three isolation methods

Skovgaard, Niels; Christensen, Sven Gade; Gulistani, A. W.

doi:10.1017/s002217240006229x

Cited by 3 publications

(8 citation statements)

References 10 publications

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“…The present data, on samples of equal weight, indicate that double selective enrichment (TTB/RV or Hajna/RV) yielded significantly superior detection than did non‐selective pre‐enrichment in BPW followed by RV enrichment. This observation is consistent with some previously published work ( Skovgaard et al . 1985 ; Cherrington and Huis in't Veld 1993) and it is suggested that the apparent contradictions in other previous studies are attributable to the use of BPW pre‐enrichment prior to enrichment in tetrathionate broths ( Bager and Petersen 1991; Cherrington and Huis in't Veld 1993).…”

Section: Discussionsupporting

confidence: 94%

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Comparison of methods for isolating Salmonella bacteria from faeces of naturally infected pigs

et al. 2000

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A series of experiments was conducted using faecal samples collected from commercial swine farms to evaluate the effects of variation in methods used for the detection of Salmonella bacteria. The primary objective of the studies was to compare the protocols routinely used in two laboratories in the USA. The studies included five experiments comparing the enrichment protocols used routinely in the respective laboratories (Method 1: 10 g faeces—buffered peptone water (BPW) pre‐enrichment—selective enrichment in Rappaport/Vassiliadis (RV) broth; Method 2: ∼1g faeces—primary enrichments in tetrathionate and Hajna GN broths—secondary enrichment in RV broth). The effects of enrichment temperatures (37 vs 42 °C) using RV broth (two experiments) and delayed secondary enrichment (four experiments) were also evaluated. Direct comparison of Method 1 and Method 2 indicated comparable results. However, when compared using faecal samples of equal weight, the Method 2 enrichment protocol was more sensitive for detecting Salmonella bacteria than the Method 1 protocol. Enrichment in RV at 42 °C was superior to 37 °C, particularly for samples that were pre‐enriched in BPW. Delayed secondary enrichment increased detection of Salmonella bacteria in swine faeces. These results highlight the imperfect sensitivity of culture methods, and the need for researchers to consider the sensitivity of bacteriological methods in the design and interpretation of the results of epidemiologic studies based on faecal culture

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Section: Discussionsupporting

confidence: 94%

“…A relatively small number of previous studies comparing enrichment broths with swine faecal (or caecal) samples has reported conflicting findings ( Skovgaard et al . 1985 ; Vassiliadis et al .…”

Section: Discussionmentioning

confidence: 99%

Comparison of methods for isolating Salmonella bacteria from faeces of naturally infected pigs

et al. 2000

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“…The discrepancies between our results and those of Skovgaard et al (1985) are difficult to explain. They may be due in part to the fact that they did not use the P/RV procedure but only the PT/RV method.…”

Section: Dis(c'ussioncontrasting

confidence: 97%

“…(PT/MKT, Oxoid and PT/MKT, h.p.i.). All PT/MKT tubes were incubated at 37°C for 48 hn as recommended by Skovgaard et al (1985).…”

Section: Methodsmentioning

confidence: 99%

“…Recently Skovgaard et al (1985) compared the efficiency of the RappaportVassiliadis medium (RV) with Muller-Kauffmann tetrathionate broth (MK) to which 1% of Teepol 610 (BDH) was added (MKT). Both these enrichment media were inoculated with a pre-enrichment culture in buffered peptone water (P) to which, also, 1% Teepol was added (PT/RV and PT/MKT pre-enrichment methods).…”

Section: Dis(c'ussionmentioning

confidence: 99%

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Comparison of procedures based upon Rappaport–Vassiliadis medium with those using Muller–Kauffmann medium containing Teepol for the isolation ofSalmonellasp.

et al. 1987

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SUMMARYA total of 308 samples of different types were examined for the presence of salmonellas by means of three different procedures. The first consisted of preenrichment in buffered peptone water followed by enrichment in RappaportVassiliadis medium (P/RV). The second differed only in that 1% Teepol was added to the pre-enrichment medium (PT/RV). In the third, buffered peptone water with 1 % Teepol was followed by enrichment in Muller-Kauffmann tetrathionate broth also containing 1 % Teepol (PT/MKT). The first of these combinations (P/RV) proved superior to the others both in terms of isolation rates and in the appearance of suspicious colonies.

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Herd prevalence of Salmonella enterica infections in Danish slaughter pigs determined by microbiological testing

Baggesen¹,

Wegener²,

Bager

et al. 1996

Preventive Veterinary Medicine

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Salmonellas in Danish pigs: a comparison of three isolation methods

Cited by 3 publications

References 10 publications

Comparison of methods for isolating Salmonella bacteria from faeces of naturally infected pigs

Comparison of methods for isolating Salmonella bacteria from faeces of naturally infected pigs

Comparison of procedures based upon Rappaport–Vassiliadis medium with those using Muller–Kauffmann medium containing Teepol for the isolation ofSalmonellasp.

Herd prevalence of Salmonella enterica infections in Danish slaughter pigs determined by microbiological testing

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