Herd prevalence of Salmonella enterica infections in Danish slaughter pigs determined by microbiological testing

Baggesen, Dorte Lau; Wegener, Henrik Caspar; Bager, Flemming; Stege, Helle; Christensen, Jette

doi:10.1016/0167-5877(95)00563-3

Cited by 80 publications

(56 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Under field conditions, the culture of faecal samples is considered to have a sensitivity below 50% [2]. In a collaborative study [12], it has been shown that the relative sensitivity of MSRV is 96% in naturally contaminated food samples.…”

Section: Discussionmentioning

confidence: 99%

Discrepancies between the isolation of Salmonella from mesenteric lymph nodes and the results of serological screening in slaughter pigs

et al. 2005

View full text Add to dashboard Cite

-Most Salmonella control programmes are based on serological testing in the slaughterhouse. However, from a point of view of carcass contamination, it is rather the presence of Salmonella spp. in the animal at the time of slaughter that is important. The aim of this crosssectional study was to investigate the possible discrepancies between the isolation of Salmonella spp. in the mesenteric lymph nodes and the results of serological screening. In total, 1821 fattening pigs originating from 60 Belgian farrow-to-finish herds were sampled in the slaughterhouse. The serum samples were analysed using an indirect mix-ELISA for the presence of Salmonella antibodies and evaluated at 3 cut-off values namely 10, 20, and 40% Optical Density (OD). All mesenteric lymph node samples were submitted to qualitative Salmonella isolation and a representative number of isolates was serotyped. From each herd, 30 animals were screened both serologically and bacteriologically and the herd was considered as positive when at least one sample was positive. At the herd level, 83.6% (cut-off OD 40%) to 100.0% (cut-off OD 10%) of the herds from which Salmonella had been isolated were evaluated as seropositive. At the individual level, only 34.5% (cut-off OD 40%) to 82.8% (cut-off OD 10%) of the animals from which Salmonella had been isolated were seropositive. Overall, a weak agreement was found between bacteriology and serology for Salmonella diagnosis. If pig herds are categorised using serological tests in the slaughterhouse, one should be aware of the fact that slaughter pigs can still harbour Salmonella spp. in the mesenteric lymph nodes, without being detected in serological tests. The cut-off value used to evaluate a sample as serologically positive and the number of samples per herd are of major importance to classify herds correctly in order to protect human health.slaughter pig / Salmonella / bacteriology / serology * Corresponding author: nathalie.nollet@UGent.be Article published by EDP Sciences and available at

show abstract

Section: Discussionmentioning

confidence: 99%

Discrepancies between the isolation of Salmonella from mesenteric lymph nodes and the results of serological screening in slaughter pigs

et al. 2005

View full text Add to dashboard Cite

show abstract

“…En los serovares de origen animal predominan S. infantis y S. Grupo E (3,9;-;-) aisladas desde cerdos, lo cual indica que estos serovares en las distintas etapas de producción no son capaces de establecerse de forma endémica a diferencia de S. thyphimurium, S. cholerasuis o S. Derby entre otros 24 . Hay que considerar también que algunos de los serovares analizados son de origen silvestre, aislados a partir de gaviotas, las cuales pueden actuar como diseminadoras de estos agentes hacia otros animales, incluido el hombre, o bien hacia alimentos.…”

Section: Discussionunclassified

Estudio de susceptibilidad antimicrobiana de Salmonella enterica en muestras de origen animal y alimentario

Junod¹,

López-Martín²,

Gädicke³

2013

Rev. méd. Chile

View full text Add to dashboard Cite

“…8 In the Danish pig production system, S. typhimurium O: 1, 4, 5, 12 is the dominating serotype found in naturally Salmonella-infected pigs (61% of 302 infected herds), 1 followed by smaller proportions of various serotypes.…”

mentioning

confidence: 99%

Evaluation of a Novel Enzyme-Linked Immunosorbent Assay for Detection of Antibodies against Salmonella, Employing a Stable Coating of Lipopolysaccharide-Derived Antigens Covalently Attached to Polystyrene Microwells

Wiuff¹,

Jauho²,

Stryhn³

et al. 2000

J VET Diagn Invest

View full text Add to dashboard Cite

Abstract. Polysaccharides derived from Salmonella typhimurium lipopolysaccharide (LPS) representing the O-antigen factors 1, 4, 5, and 12 and the O-antigen factors 6 and 7 from Salmonella choleraesuis LPS were derivatized with the photoreactive compound anthraquinone and subsequently covalently coupled to microtiter polystyrene plates by ultraviolet irradiation. Both polysaccharide antigens could be coupled simultaneously to the same microtiter plate. The coated surface was used in indirect ELISA for the determination of serum antibodies from pigs infected with bacteria of the two Salmonella groups and from uninfected pigs. This ELISA proved itself by having a good long-term durability and a high degree of reproducibility, including low dayto-day variations and low interplate variations. Furthermore, the ELISA showed good specificity and sensitivity when data were compared with the optical density levels of a panel of pig sera as determined by a conventional ELISA on the basis of passive coating of the two Salmonella LPS antigens (the mix-ELISA). The covalent anthraquinone mix-ELISA shows promise as a stable and durable alternative to the existing conventional ELISA for serological surveillance of Salmonella infections in pigs.Bacterial lipopolysaccharides (LPSs) are important serotype-defining antigens of gram-negative bacteria, 17 and they are often, therefore, used as antigens in serological tests for the detection of serotype-specific antibodies. 2,10 Lipopolysaccharide consists of the hydrophobic lipid A part and the hydrophilic polysaccharide (PS) part. The latter can be further subdivided into a constant core region roughly similar in all Enterobacteriaceae and the variable O-antigenic PS specific for the bacterial serotype. 4,13 Lipopolysaccharide is anchored to the outer bacterial membrane by lipid A, whereas the highly immunoreactive PS part is exposed and able to interact with the immune system of the infected host. 17 In conventional solid-phase immunoassays (e.g., ELISA) for detection of anti-LPS antibodies, purified LPS is passively adsorbed to the plastic surface by hydrophobic interactions between the lipid A and the plastic surface, leaving the antigenic PS exposed to the solvent and freely accessible to the antibodies. However, coating by passive adsorption is strongly dependent on the overall amphiphilicity of the LPS, being in turn dependent on the specific type of LPS, and because purified LPS is heterogeneous because of the From the Danish Veterinary Laboratory,

show abstract

Herd prevalence of Salmonella enterica infections in Danish slaughter pigs determined by microbiological testing

Cited by 80 publications

References 19 publications

Discrepancies between the isolation of Salmonella from mesenteric lymph nodes and the results of serological screening in slaughter pigs

Discrepancies between the isolation of Salmonella from mesenteric lymph nodes and the results of serological screening in slaughter pigs

Estudio de susceptibilidad antimicrobiana de Salmonella enterica en muestras de origen animal y alimentario

Evaluation of a Novel Enzyme-Linked Immunosorbent Assay for Detection of Antibodies against Salmonella, Employing a Stable Coating of Lipopolysaccharide-Derived Antigens Covalently Attached to Polystyrene Microwells

Contact Info

Product

Resources

About