Salmonella Typhimurium metabolism affects virulence in the host – A mini-review

Herrero-Fresno, Ana; Olsen, John Elmerdahl

doi:10.1016/j.fm.2017.04.016

Cited by 61 publications

(34 citation statements)

References 110 publications

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“…Macrophage ROS response results in DNA damage in the bacteria and to repair this damage it is crucial to have functional purine biosynthesis. This pathway also regulates Sif formation and SPI-2 T3SS formation [86].…”

Section: Metabolismmentioning

confidence: 99%

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Hoodwinking the Big-Eater to Prosper: The Salmonella-Macrophage Paradigm

2018

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Salmonella is a major cause of morbidity and mortality in the developing and underdeveloped nations. Being a foodborne disease, Salmonella infection is primarily contracted through the ingestion of contaminated food or water, or due to close contact with infected/carrier individuals. It is an intracellular pathogen, which can survive and replicate in various cells including macrophages, dendritic cells, epithelial cells, and other white blood cells. Once Salmonella crosses the intestinal barrier, it disseminates to various systemic sites by circulation via immune cells. One of the major cell types which are involved in Salmonella infection are host macrophages. They are the niche for intracellular survival and proliferation of Salmonella and a mode of dissemination to distal systemic sites. These cells are very crucial as they mediate the mounting of an appropriate innate and adaptive anti-Salmonella immune response. In this review, we have tried to concise the current knowledge of complex interactions that occur between Salmonella and macrophages.

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Section: Metabolismmentioning

confidence: 99%

“…Instead, it depends on substrate level phosphorylation for ATP generation rather than proton gradient [86]. Salmonella has been reported to use host amino acids for its survival.…”

Section: Metabolismmentioning

confidence: 99%

Hoodwinking the Big-Eater to Prosper: The Salmonella-Macrophage Paradigm

2018

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“…These include genes that encode virulence factors, usually grouped in pathogenicity islands ( Fookes et al, 2011 ; Kaur and Jain, 2012 ; Nieto et al, 2016 ), as well as, the presence of virulence plasmid p SLT, fimbriae, flagellum, in addition to the capacity to resist the stresses found in the host ( Fabrega and Vila, 2013 ; Runkel et al, 2013 ). Besides, the ability to adjust the metabolism to adapt to the physical conditions and available nutrients found in the host during infection seems to be fundamental for the success of Salmonella as a pathogen ( Dandekar et al, 2015 ; Rivera-Chávez and Andreas, 2015 ; Herrero-Fresno and Olsen, 2018 ). In addition, the regulation of these phenotypes can occur in a synchronized way, through a cellular communication mechanism called quorum sensing (QS).…”

Section: Introductionmentioning

confidence: 99%

Salmonella enterica Optimizes Metabolism After Addition of Acyl-Homoserine Lactone Under Anaerobic Conditions

et al. 2020

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Acyl-homoserine lactones (AHLs) are quorum sensing (QS) signaling molecules that mediate cell-to-cell communication in Gram-negative bacteria. Salmonella does not produce AHL, however, it can recognize AHLs produced by other species through SdiA protein modulating important cellular functions. In this work, the influence of the N -dodecanoyl-DL-homoserine lactone (C12-HSL) on glucose consumption, metabolic profile, and gene expression of Salmonella throughout the cultivation time in Tryptic Soy Broth (TSB) under anaerobic conditions was evaluated. Analysis of the supernatant culture in high-performance liquid chromatography (HPLC) revealed lower glucose uptake after 4 and 6 h of the addition of C12-HSL. Gas chromatography-mass spectrometry (GC-MS) based analysis of the intracellular metabolites revealed C12-HSL perturbation in the abundance levels of metabolites related to the metabolic pathways of glycerolipids, purines, amino acids, and aminoacyl-tRNA biosynthesis. The real-time quantitative PCR (RT-qPCR) indicated that Salmonella increase expression of genes associated with nucleoside degradation and quantification of metabolites supported the induction of pentose phosphate pathway to ensure growth under lower glucose consumption. The obtained data suggest an important role of C12-HSL in the optimization of metabolism at a situation of high population densities.

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“…The DSS modeling method we often use has certain defects for the study of bacterial infections and intestinal flora, so in the present study we employed the bacteria-induced colitis model. Salmonella typhimurium, a food source for Gram-negative bacilli, can cause human gastroenteritis [30]. The mechanism of inducing intestinal inflammation may involve the expression of T-cell-dependent cytokines such as IFN-gamma, IL-22 and IL-17, and IL-23 plays a very important role in initiating this immune response [31].…”

Section: Introductionmentioning

confidence: 99%

VDR Pathway-Specific Dose-Related Effects of 1,25(OH)2D3 on Salmonella typhimurium-induced Mouse Colitis

Qiu¹,

Zhang²,

Li³

et al. 2020

Preprint

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Background: Whether and how 1,25(OH)2D3 supplementation influences VDRs in experimental mice with colitis remains to be seen. To explore the effect of 1,25(OH)2D3 on S. typhimurium colitis through the VDR pathway and to discover the role of VDR in its action. Methods: We established a mouse UC model induced by S. typhimurium. After streptococcal typhus infection, the mice were fasted for 12 hours. Blood was collected by the eyeball extraction method, and then sacrificed by cervical dislocation, specimens were collected for corresponding indicators. Results: Mice exposed to S. typhimurium infection developed signs of acute colitis. After HE staining were performed on the diseased colons from the mice. high dose VD supplementation, the pathological colonic damage did not improve in the mice, and there was no statistical difference between the groups with VD deficiency (P>0.05). VDR expression in the UC group treated with Salmonella was higher than that in the control group, a statistically significant difference (P<0.01). Compared with the VDD+UC group, VDR expression rose in both the LVDS+UC group and the HVDS+UC group, with VDR protein expression being highest after high dose VD supplementation (P<0.01). Compared with the control and the UC groups, the VDR mRNA expression level in the VDD+UC group was significantly higher, and the colon VDR mRNA expression level decreased after active VD supplementation (Fig.7C). Conclusions: Our data suggest the need for defining the accurate 1,25(OH)2D3 dose limits that induce an anti-inflammatory effect as current data indicate that higher doses would produce an inflammatory response.

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Salmonella Typhimurium metabolism affects virulence in the host – A mini-review

Cited by 61 publications

References 110 publications

Hoodwinking the Big-Eater to Prosper: The <b><i>Salmonella</i></b>-Macrophage Paradigm

Hoodwinking the Big-Eater to Prosper: The <b><i>Salmonella</i></b>-Macrophage Paradigm

Salmonella enterica Optimizes Metabolism After Addition of Acyl-Homoserine Lactone Under Anaerobic Conditions

VDR Pathway-Specific Dose-Related Effects of 1,25(OH)2D3 on Salmonella typhimurium-induced Mouse Colitis

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