Salmonella enterica Optimizes Metabolism After Addition of Acyl-Homoserine Lactone Under Anaerobic Conditions

Carneiro, Deisy Guimarães; Almeida, Felipe Alves de; Aguilar, Ananda Pereira; Vieira, Nívea Moreira; Pinto, Uelinton Manoel; Mendes, Tiago Antônio de Oliveira; Vanetti, Maria Cristina Dantas

doi:10.3389/fmicb.2020.01459

Cited by 12 publications

(5 citation statements)

References 58 publications

(65 reference statements)

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“…However, at the population level, the maintenance of bacterial metabolic homeostasis is important for energy conservation and resource utilization [23]. Recent reports have detailed the role of AHL-mediated QS in controlling nutrient intake and aiding in maintaining primary metabolism homeostasis in Burkholderia glumae [24], Pseudomonas aeruginosa [23], and Yersinia pestis [25]. Here, we propose a new mechanism for the regulation of cell metabolism mediated by the AI-2 QS systems.…”

Section: Discussionmentioning

confidence: 93%

Autoinducer‐2‐mediated quorum‐sensing system resists T4 phage infection in Escherichia coli

et al. 2021

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In response to the restriction of nutrients and predation by natural enemies, bacteria have evolved complex coping strategies to ensure the reproduction and survival of individual species. Quorum sensing (QS) is involved in the bacterial response to phage predation and regulation of cellular metabolism. However, to date, no clear evidence exists regarding the involvement of autoinducer‐2 (AI‐2)‐mediated QS systems in Escherichia coli in response to the challenges of nutrient restriction and phage infection. In this study, the role of the AI‐2‐mediated QS system in resisting T4 phage infection and regulating cell mechanisms in E. coli was revealed for the first time. This effect of the AI‐2‐mediated QS was achieved by simultaneously downregulating the T4 absorption site and carbon and glucose metabolism. Additionally, we found that lsrB, a metabolic brake, participates in AI‐2‐mediated regulation and maintenance of the normal metabolic balance of cells. The novel phage defense strategy and regulation and maintenance of cellular metabolism effectively limited the expansion of the phage population.

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Section: Discussionmentioning

confidence: 93%

Autoinducer‐2‐mediated quorum‐sensing system resists T4 phage infection in Escherichia coli

et al. 2021

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“…7 , first panel. A high concentration of signaling molecules would suggest an overcrowded environment, so organisms can adapt and reduce their metabolism for a possible substrate shortage ( 53 ). PICRUSt2 predicts possible functions of a community and must not be taken as a proof of actual metabolic activity.…”

Section: Discussionmentioning

confidence: 99%

Joining the bacterial conversation: increasing the cultivation efficiency of soil bacteria with acyl-homoserine lactones and cAMP

Lopez Marin,

Strejcek,

Uhlik

2023

Microbiol Spectr

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Bacteria need to be isolated in pure culture to gain access to the wide array of interesting functions they conceal. This is challenging because they do not live in isolation but instead in communities where they actively communicate and interact with each other. In this study, we aimed to increase the culturability of soil bacteria using three different signaling molecules: N-(3-oxohexanoyl)-L-homoserine lactone, N-octanoyl-L-homoserine lactone, and 3',5'-cyclic adenosine monophosphate (cAMP). The signals were added individually to soils suspended in PBS buffer to a final concentration of 5 µM. Soil suspensions were agitated for 24 hours, after which they were serially diluted and plated on tenfold-diluted Reasoner’s 2A agar that either contained or did not contain the same signaling molecule used during the extraction step. DNA was isolated from both soil suspensions and grown cultures and, after a high-throughput amplicon sequencing, differences in bacterial abundances and diversity were determined across treatments. To further explain the action of the signaling molecules on the treated soil communities, their metagenomic functions were predicted using the software PICRUSt2. N-octanoyl-L-homoserine lactone was found to increase the diversity observed on solid media, while N-(3-oxohexanoyl)-L-homoserine lactone and cAMP were not. Potentially novel isolates aided by the signaling molecules were affiliated with the genera Pseudomonas and Nocardioides . IMPORTANCE Microorganisms are a repository of interesting metabolites and functions. Therefore, accessing them is an important exercise for advancing not only basic questions about their physiology but also to advance technological applications. In this sense, increasing the culturability of environmental microorganisms remains an important endeavor for modern microbiology. Because microorganisms do not live in isolation in their environments, molecules can be added to the cultivation strategies to “inform them” that they are present in growth-permissive environmental conditions. Signaling molecules such as acyl-homoserine lactones and 3',5'-cyclic adenosine monophosphate belong to the plethora of molecules used by bacteria to communicate with each other in a phenomenon called quorum sensing. Therefore, including quorum sensing molecules can be an incentive for microorganisms, specifically soil bacteria, to increase their numbers on solid media.

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“…In addition, amplification of SdiA can enhance resistance to mitomycin C by increasing the ability of chromosome replication and repair. Carneiro et al (2020) examined the expression of SdiA and genes associated with the nucleotide salvage pathway in Salmonella by qRT-PCR and found that SdiA affects glucose consumption, metabolism and gene expression in Salmonella and is closely related to metabolic pathways associated with glycine, purine, amino acid and aminoacyl tRNA biosynthesis. It plays a role in metabolic optimization under a high population density of Salmonella.…”

Section: Discussionmentioning

confidence: 99%

SdiA Enhanced the Drug Resistance of Cronobacter sakazakii and Suppressed Its Motility, Adhesion and Biofilm Formation

et al. 2022

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Cronobacter sakazakii is a common foodborne pathogen, and the mortality rate of its infection is as high as 40–80%. SdiA acts as a quorum sensing regulator in many foodborne pathogens, but its role in C. sakazakii remains unclear. Here, we further determined the effect of the sdiA gene in C. sakazakii pathogenicity. The SdiA gene in C. sakazakii was knocked out by gene editing technology, and the biological characteristics of the ΔsdiA mutant of C. sakazakii were studied, followed by transcriptome analysis to elucidate its effects. The results suggested that SdiA gene enhanced the drug resistance of C. sakazakii but diminished its motility, adhesion and biofilm formation ability and had no effect on its growth. Transcriptome analysis showed that the ΔsdiA upregulated the expression levels of D-galactose operon genes (including dgoR, dgoK, dgoA, dgoD and dgoT) and flagella-related genes (FliA and FliC) in C. sakazakii and downregulated the expression levels of related genes in the type VI secretion system (VasK gene was downregulated by 1.53-fold) and ABC transport system (downregulated by 1.5-fold), indicating that SdiA gene was related to the physiological metabolism of C. sakazakii. The results were useful for clarifying the pathogenic mechanism of C. sakazakii and provide a theoretical basis for controlling bacterial infection.

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Salmonella enterica Optimizes Metabolism After Addition of Acyl-Homoserine Lactone Under Anaerobic Conditions

Cited by 12 publications

References 58 publications

Autoinducer‐2‐mediated quorum‐sensing system resists T4 phage infection in Escherichia coli

Autoinducer‐2‐mediated quorum‐sensing system resists T4 phage infection in Escherichia coli

Joining the bacterial conversation: increasing the cultivation efficiency of soil bacteria with acyl-homoserine lactones and cAMP

SdiA Enhanced the Drug Resistance of Cronobacter sakazakii and Suppressed Its Motility, Adhesion and Biofilm Formation

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