Salmonella purification v1

Justiz-Vaillant, Angel; Curtello, Suzette E

doi:10.17504/protocols.io.bjjekkje

Cited by 5 publications

(10 citation statements)

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“…It has also been reported that the underlying pathophysiology of chronic graft-versus-host disease (GVHD) is similar to autoimmune diseases like scleroderma [ 6 ]. GVHD remains in the differential, but the sites of involvement are usually the GI tract, liver, and skin, with lungs being less commonly involved [ 15 ]. The diagnosis of GPA can be challenging.…”

Section: Discussionmentioning

confidence: 99%

Cytoplasmic Antineutrophil Cytoplasmic Antibodies (C-ANCA) Vasculitis: An Uncommon Complication After Stem Cell Transplantation

Ahmad¹,

Afridi²,

Noe³

et al. 2022

Cureus

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Granulomatosis with polyangiitis (GPA) is a rare, autoimmune, antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis of uncertain etiology. The incidence of autoimmune complications following stem cell transplant is around 2-5%, with autoimmune cytopenia reported most frequently. We present a case of a 65-year-old male patient who presented to the hospital with productive cough, dyspnea, and fever for five months after haploidentical stem cell transplantation. On presentation, he was febrile, tachypneic, and mildly hypoxic. Chest radiograph showed bilateral pulmonary infiltrates. An initial diagnosis of pneumonia was made, and the patient was started on antibiotics. The patient did not respond to initial management, and all his initial infectious workups came back negative. On further evaluation, cytoplasmic antineutrophil cytoplasmic antibodies (c-ANCA) resulted positive in high titers. The patient was diagnosed with GPA, and IV methylprednisolone and rituximab were started. He responded well to treatment and was eventually discharged home. The classical form of GPA is characterized by the involvement of the upper respiratory tract, sinuses, lungs, and kidneys. Autoimmune disorders may develop secondary to hematopoietic stem cell transplant (HSCT). In our case, the patient was diagnosed with GPA, which is likely one of the autoimmune complications after HSCT.

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Section: Discussionmentioning

confidence: 99%

Cytoplasmic Antineutrophil Cytoplasmic Antibodies (C-ANCA) Vasculitis: An Uncommon Complication After Stem Cell Transplantation

Ahmad¹,

Afridi²,

Noe³

et al. 2022

Cureus

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“…The plate was assessed visually for color development and read on a microplate reader at 450 nm. Subsequently, a cut-off point is calculated as the mean of the optical density of the negative controls multiplied by two [13]. The cut-off point was set to 0.268.…”

Section: Spla-lg Direct Elisamentioning

confidence: 99%

“…Table 1 shows that SpLA-LG direct ELISA is a novel immunoassay that examines the binding affinity of a novel hybrid immunoglobulin-binding protein to immunoglobulins from a variety of animal species [10,13]. The authors are not aware of the development of an ELISA using this protein conjugate for immunodetection.…”

Section: Spla-lg Direct Elisamentioning

confidence: 99%

“…This ELISA was used to study the interaction of SpLAG-anti-IgY-HRP proteins with avian and mammalian immunoglobulins or antibodies [10,13]. The 96 well microtiter plate was coated overnight at 4°C with 1 µg/µl per well of IgG or IgY (1 mg/mL) in carbonate-bicarbonate buffer (pH 9.6).…”

Section: Splag-anti-igy Direct Elisamentioning

confidence: 99%

“…The microwells were visually assessed for color development and read on a microplate reader at 450 nm. Subsequently, a cut-off point was calculated as the mean of the optical density of the negative controls multiplied by three [13,14]. The higher the OD value, the higher the binding affinity of the bacterial proteins to immunoglobulins.…”

Section: Splag-anti-igy Direct Elisamentioning

confidence: 99%

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Universal Enzyme-Linked Immunosorbent Assays (ELISA) and Utility in the Detection of Antibodies against Salmonella spp. in Several Animal Species

Justiz-Vaillant

Ferrer-Cosme²,

Curtello

2022

IJTDH

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The aim of this study was to confirm the feasibility of using hybrid immunoglobulin-binding reagents in ELISAs for IgG/IgY detection and detection of specific antibodies against an infectious microorganism (Salmonella spp.) in various animal species using a universal diagnostic ELISA. Hybrid immunoglobulin-binding bacterial proteins (IBP), including recombinant protein LA, recombinant protein LG, and recombinant protein AG, have been produced to improve their binding affinity to a much larger number of immunoglobulins. Thus, this hybrid bacterial protein represents a powerful tool for the binding, detection, and purification of immunoglobulins and their fragments. However, SpLA-LG-peroxidase and SpLAG-anti-IgY-peroxidase were produced using the periodate method. These compounds have been shown to be effective as reagents. Their binding affinity to immunoglobulins surpasses that of previously reported hybrid IgG-binding proteins, including the most known SpAG, SpLA, and SpLG. The IgY fraction was isolated from the egg yolks of various birds, including chicken, bantam hen, guinea hen, quail, goose, duck, wild and domestic pigeons, parakeets, cattle egrets, pheasants, and ostrich. The IgY fraction was isolated using the chloroform-polyethylene glycol (PEG) method. An ELISA for anti-Salmonella spp. antibodies was employed with some modifications to determine the presence of antibodies in humans, laying hens, geese, quails, and pigeons. Salmonella is a motile, flagellated, rod-shaped zoonotic pathogen that can survive in the presence or absence of oxygen. They belong to the family Enterobacteriaceae and are implicated in typhoid fever and food-borne illnesses. This pathogen is associated with several diseases, which may become fatal and negatively impact the health of individuals and various economies globally. The poultry industry is most vulnerable to the influence of this pernicious microbe. The authors concluded that universal enzyme-linked immunosorbent assays were effective and reproducible in detecting immunoglobulins from both avian and mammalian species, but ELISA using the SpLAG-anti-IgY-HRP conjugate only reacted with the whole panel of animal antibodies. This conjugate was further used to standardize a universal ELISA for the determination of anti-Salmonella antibodies, in which human and avian species were serologically assessed.

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Inflammatory and vascular biomarkers in post‐COVID‐19 syndrome: A systematic review and meta‐analysis of over 20 biomarkers

Yong

Halim

et al. 2023

Reviews in Medical Virology

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Severe acute respiratory syndrome coronavirus 2 may inflict a post-viral condition known as post-COVID-19 syndrome (PCS) or long-COVID. Studies measuring levels of inflammatory and vascular biomarkers in blood, serum, or plasma of COVID-19 survivors with PCS versus non-PCS controls have produced mixed findings. Our review sought to meta-analyse those studies. A systematic literature search was performed across five databases until 25 June 2022, with an updated search on 1 November 2022. Data analyses were performed with Review Manager and R Studio statistical software. Twenty-four biomarkers from 23 studies were meta-analysed. Higher levels of C-reactive protein (Standardized mean difference (SMD) = 0.20; 95%

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Salmonella purification v1

Cited by 5 publications

References 0 publications

Cytoplasmic Antineutrophil Cytoplasmic Antibodies (C-ANCA) Vasculitis: An Uncommon Complication After Stem Cell Transplantation

Cytoplasmic Antineutrophil Cytoplasmic Antibodies (C-ANCA) Vasculitis: An Uncommon Complication After Stem Cell Transplantation

Universal Enzyme-Linked Immunosorbent Assays (ELISA) and Utility in the Detection of Antibodies against Salmonella spp. in Several Animal Species

Inflammatory and vascular biomarkers in post‐COVID‐19 syndrome: A systematic review and meta‐analysis of over 20 biomarkers

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