Salmonella in Raw Chicken Meat from the Romanian Seaside: Frequency of Isolation and Antibiotic Resistance

Abstract

The purpose of this study was to investigate the occurrence of Salmonella in raw chicken meat samples collected at the Romanian seaside and to evaluate the antimicrobial susceptibility of the isolates. In 2012, 317 chicken meat samples from slaughterhouses (n = 289) and retail markets (n = 28) were evaluated. Overall, 13.2% (42) of the samples contained Salmonella; 12.8% (37) and 17.8% (5) from the chicken carcasses at slaughterhouses and the fresh meat from retail markets, respectively. Eight serotypes of Salmonella enterica subsp. enterica were identified: Infantis (18 isolates), Bredeney (7), Virchow (6), Djugu (4), Grampian (4), Brandenburg (1), Derby (1), and Ruzizi (1). The isolates were resistant to tetracycline (66.6% of isolates), nalidixic acid (64.3%), sulfamethoxazole (64.3%), ciprofloxacin (61.9%), streptomycin (59.5%), trimethoprim (33.3%), ampicillin (9.5%), chloramphenicol (7.1%), and gentamicin (2.4%). No resistance was found to cefotaxime and ceftazidime. Thirty (71.4%) of the 42 tested isolates had multidrug resistance patterns to at least two antimicrobials. This survey highlighted a multidrug-resistant Salmonella contamination rate in raw chicken meat in this area of Romania, which can seriously threaten human health.

Alternate antimicrobial resistance genes in multidrug resistant Salmonella spp. isolated from retail meats in Vietnam using RNA‐sequencing analysis

“…The high resistance rate to tetracycline and streptomycin (about 44%) was inevitable, since these antibiotics are popularly used on farms and in humans in Vietnam. This finding is in line with previous reports from Romania (Tirziu et al, ) and Tokyo (Katoh et al, ). Ceftazidime has shown to be a good antibiotic, with no Salmonella isolates being resistant all isolates.…”

“…The high prevalence of Salmonella isolates in retail meats obtained in this study was similar to that described in previous studies (Donado‐Godoy et al, ; Katoh et al, ; Takeshi et al, ). The 28% overall prevalence of Salmonella isolates observed in this study is lower than that found in other studies conducted in Spain (35.8%; Dominguez, Gomez, & Zumalacarregui, ) and Portugal (60%; Antunes, Reu, Sousa, Peixe, & Pestana, ), but higher than figures reported from Ireland (2.8%; Jordan et al, ) and Romania (13.2%; Tirziu et al, ). In general, the various levels of contamination might also be related to climate and temperature of food storage (Akil, Ahmad, & Reddy, ).…”

“…In this study, approximately 52% of the isolates were resistant to at least one antibiotic, which was higher than other studies in Poland (42.6%; Maka et al, ), but lower than figures reported in Tokyo (89.9%; Katoh et al, ) and India (67.5%; Deekshit et al, ). The overall rate of multi antimicrobial resistance was 36%, which was higher than that described in Korea (29%; Choi et al, ) and India (25%; Deekshit et al, ), but lower than figures reported in Romania (71.4%; Tirziu et al, ), Japan (90.2%; Katoh et al, ), and Colombia (98%, Donado‐Godoy et al, ). The cause of this difference could be attributed to the abuse of antibiotics used in animal feed, used in treatment and prevention of livestock diseases on farms, and use in treatment of human patients, all of which has increased the rate of resistance genes for microorganisms (Miranda, Mondragon, Martinez, Guarddon, & Rodriguez, ).…”

Presencia y Resistencia a los Antimicrobianos de serovariedades de Salmonella enterica aisladas en una empresa avícola integrada del Ecuador

“…Así, se han encontrado cepas resistentes de Salmonella en diferentes etapas de la cadena productiva avícola. Dichas cepas han sido aisladas de muestras de granja, contenido cecal, carcasas y carne de pollo (Mainali et al 2014, Tîrziu et al 2015, Voss-Rech et al 2015, Cui et al 2016.…”

Absorbent Food PADS from Meat Packages-Potential Source of Contamination

“…Also, along with Salmonella kentucky, it was the most commonly reported serovar showing multidrug resistance [14]. Both nationally and in Europe, it has been the most frequently identified serovar in chicken meat in recent years [14,25,38].…”