Salmonella Heidelberg Isolated from Poultry Shows a Novel Resistance Profile

Neves, Gabriella Bassi das; Stefani, Lenita M.; Pick, Eduarda; Araujo, Denise Nunes; Giuriatti, Jessica; Percio, Cleverson; Brisola, Maiara Cristiane

doi:10.22456/1679-9216.81294

Cited by 9 publications

(12 citation statements)

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“…As already reported by Neves et al [14] and Krumperman [7], the present study shows that SH is a real challenge regarding antimicrobial resistance when tested against antibiotics routinely used in the treatment of samonelosis in veterinary and human medicine. These authors obtained similar results for the different classes of antimicrobials tested, a fact that may also be related to horizontal transmission of resistance genes.…”

Section: Discussionsupporting

confidence: 77%

Salmonella Heidelberg: Genetic profile of its antimicrobial resistance related to extended spectrum β-lactamases (ESBLs)

Giuriatti

Stefani

Brisola

et al. 2017

Microbial Pathogenesis

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The objective of this study was to evaluate the phenotypic and genotypic profile of antimicrobial susceptibility and the possible involvement of extended spectrum beta-lactamases (ESBLs) in the resistance profile of Salmonella Heidelberg (SH) isolated from chicken meat. We used 18 SH isolates from chicken meat produced in 2013 in the state of Paraná, Southern Brazil. The isolates were submitted to disk-diffusion tests and from these results it was possible to determine the number of isolates considered multiresistant and the index of multiple antimicrobial resistance (IRMA) against ten antimicrobials routinely used in human and veterinary medicine. It was considered multidrug resistant the isolate that showed resistance to three or more classes of antibiotics. Another test performed was the disc-approximation in order to investigate interposed zones of inhibition, indicative of ESBLs production. In the isolates that presented multidrug resistance (18/18), a search of resistance genes involved in the production of ESBLs was performed using PCR: blaCMY-2, blaSHV-1, blaTEM-1, blaCTX-M2, blaOXA-1, blaPSE-1 and AmpC. The overall antimicrobial resistance was 80.55%. The highest levels of resistance were observed for nalidixic acid and ceftiofur (100%). The most commonly resistance pattern found (42.1%) was A (penicillin-cephalosporin-quinolone-tetracycline). The results were negative for ghost zone formation, indicative of ESBLs. However, PCR technique was able to detect resistance genes via ESBLs where the blaTEM-1 gene showed the highest amplification (83.33%), and the second most prevalent genes were blaCMY-2 (38.88%) and AmpC gene (38.88%). The blaOXA-1 and blaPSE-1 genes were not detected. These results are certainly of concern since SH is becoming more prevalent in the South of Brazil and able to cause severe disease in immune compromised individuals, showing high antimicrobial resistance to those drugs routinely used in the treatment and control of human and animal salmonellosis.

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Section: Discussionsupporting

confidence: 77%

Salmonella Heidelberg: Genetic profile of its antimicrobial resistance related to extended spectrum β-lactamases (ESBLs)

Giuriatti

Stefani

Brisola

et al. 2017

Microbial Pathogenesis

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“…The previous studies have reported that Salmonella spp. strains isolated from animal products were MDR strains in Colombia [ 22 ] and Brazil [ 23 ]. In the present study, we detected a high percentage of Salmonella strains that were phenotypically resistant to STR (79.5%); these findings are consistent with the previous studies that reported that strains isolated from broiler farms in Cundinamarca (Colombia) [ 22 ] and in the United States [ 24 ] exhibited a high percentage of resistance to STR.…”

Section: Discussionmentioning

confidence: 99%

“…The previous studies have reported that Salmonella spp. strains isolated from animal products were MDR strains in Colombia [22] and Brazil [23].…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of antimicrobial resistance and enterobacterial repetitive intergenic consensus-PCR as a molecular typing tool for Salmonella spp. isolated from poultry and humans

2020

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Background and Aim: Salmonella spp. are one of the most important food-borne pathogens in the world, emerging as a major public health concern. Moreover, multidrug-resistant (MDR) strains have been isolated from salmonellosis outbreaks, which compromise its treatment success. This study was conducted to characterize the phenotypic and genotypic antibiotic resistance profile of Salmonella strains isolated from broilers and humans from the regions of Tolima and Santander (Colombia). Materials and Methods: Salmonella spp. strains (n=49) were confirmed through molecular detection by amplification of the invA gene. Phenotypic antibiotic resistance was determined by the automated method and the agar diffusion method, and the presence of resistance genes was evaluated by PCR. Genotypic characterization was conducted using the enterobacterial repetitive intergenic consensus (ERIC)-PCR method, from which a dendrogram was generated and the possible phylogenetic relationships were established. Results: Salmonella isolates were classified as MDR strains exhibiting resistance to four antibiotic classes, penicillins, aminoglycosides, sulfonamides, and cephalosporins, and the human strains were resistant to gentamicin. At the genotypic level, the isolates contained the genes blaCMY2, blaCTX-M, blaPSE-1, blaTEM, aadA1, srtB, dfrA1, sul2, and floR. The genotyping results obtained by ERIC-PCR allowed the grouping of strains according to the source of isolation. Conclusion: The Salmonella spp. strains exhibited resistance to multiple antibiotics, as well as multiple genes associated with them, and the ERIC-PCR method was a technique that was helpful in generating clusters with biological significance.

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“…Another important fact related to Salmonella spp. is the constant description of resistance to various antimicrobials, such as those of the quinolone class (Duarte et al 2009, Mion et al 2014, Casas et al 2016, Neves et al 2016, Rodrigues et al 2017. Resistance in Salmonella spp.…”

Section: Introductionmentioning

confidence: 99%

Quinolones resistance in Salmonella spp. isolated from broilers and chickens’ carcasses under federal inspection

et al. 2020

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We analyzed 77 Salmonella spp. strains, from which 20 were isolated from broilers (cloacal swabs) and 57 from chickens from slaughterhouses under federal inspection. The following serotypes were identified: Salmonella Saint Paul (29), Salmonella Heidelberg (27), Salmonella Anatum (9), Salmonella Cerro (5), Salmonella Senftenberg (5), Salmonella enterica (O: 4,5) (1) and Salmonella enterica (O: 9.12) (1). Fifteen strains (19.5%) were resistant to enrofloxacin, six (7.8%) to ciprofloxacin, and 26 (33.8%) to nalidixic acid in the Disk Diffusion Test. The fifteen enrofloxacin resistant strains were selected for the PCR to detect the genes gyrA, gyrB, parC, and parE, and genetic sequencing to identify mutations in these genes. Five strains (33.3%) had point mutations in the gyrA gene, and one (6.7%) presented a point mutation in the parC gene. None of the 15 strains had mutations in the gyrB and parE genes, and none had more than one mutation in the gyrA gene or the other genes. The presence of point mutations in the strains studied corroborates with the phenotypic resistance observed to nalidixic acid. However, it did not explain the resistance to fluoroquinolones found in the 15 strains. Other mechanisms may be related to the fluoroquinolones resistance, highlighting the need for additional mutation screening.

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Salmonella Heidelberg Isolated from Poultry Shows a Novel Resistance Profile

Cited by 9 publications

References 10 publications

Salmonella Heidelberg: Genetic profile of its antimicrobial resistance related to extended spectrum β-lactamases (ESBLs)

Salmonella Heidelberg: Genetic profile of its antimicrobial resistance related to extended spectrum β-lactamases (ESBLs)

Molecular characterization of antimicrobial resistance and enterobacterial repetitive intergenic consensus-PCR as a molecular typing tool for Salmonella spp. isolated from poultry and humans

Quinolones resistance in Salmonella spp. isolated from broilers and chickens’ carcasses under federal inspection

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