Quinolones resistance in Salmonella spp. isolated from broilers and chickens’ carcasses under federal inspection

Abstract

We analyzed 77 Salmonella spp. strains, from which 20 were isolated from broilers (cloacal swabs) and 57 from chickens from slaughterhouses under federal inspection. The following serotypes were identified: Salmonella Saint Paul (29), Salmonella Heidelberg (27), Salmonella Anatum (9), Salmonella Cerro (5), Salmonella Senftenberg (5), Salmonella enterica (O: 4,5) (1) and Salmonella enterica (O: 9.12) (1). Fifteen strains (19.5%) were resistant to enrofloxacin, six (7.8%) to ciprofloxacin, and 26 (33.8%) to nalidixic acid in the Disk Diffusion Test. The fifteen enrofloxacin resistant strains were selected for the PCR to detect the genes gyrA, gyrB, parC, and parE, and genetic sequencing to identify mutations in these genes. Five strains (33.3%) had point mutations in the gyrA gene, and one (6.7%) presented a point mutation in the parC gene. None of the 15 strains had mutations in the gyrB and parE genes, and none had more than one mutation in the gyrA gene or the other genes. The presence of point mutations in the strains studied corroborates with the phenotypic resistance observed to nalidixic acid. However, it did not explain the resistance to fluoroquinolones found in the 15 strains. Other mechanisms may be related to the fluoroquinolones resistance, highlighting the need for additional mutation screening.

Análise Genômica Das Bactérias Resistentes Mais Recorrentes Relacionadas a Pneumonia Adquirida Em Hospitais

“…Paralelamente, Cabrera et al, (2020), apresenta em seu estudo uma ampla variedade de genes e identificação de mutações nos mesmos, como identificado no complexo gyrA, gyrB (DNA girasse), grlA e grlB (topoisomerase IV) responsáveis por codificarem essas proteínas, que são alvos do ciprofloxacina (Carneiro et al, 2020) (Eichenberger et al, 2019). Diante disso, como observado no quadro 03, Kazemian et al, (2019), realizou um estudo com 90 amostras, onde os resultados apresentados, detinham 36 cepas com genes de EBLs, como bla TEM; bla SHV; bla CTX-M; genes associados a AmpC foram detectados em 22 isolados, sendo esses bla DHA , bla CIT; bla EBC e genes produtores de carbapenemases foram relacionados ao achados de blaVIM; bla OXA-48; bla IMP;, sendo encontrados em 34 cepas.…”