Salmonella flagellin is a potent carrier–adjuvant for peptide conjugate to induce peptide-specific antibody response in mice

Salmonella flagellin acted as an effective fusion partner for expression of Plasmodium falciparum surface protein 25 in Escherichia coli

“…1F). The mouse anti-rFliC immune sera (made in our previous study 7 ) didn't recognize yPfs25. The results suggested that the Pfs25 as a fused protein within FliC-Pfs25 had a conformation, to some extent, similar to that of yPfs25.…”

“…6 In addition, Salmonella flagellin presents some distinct properties that make it a potential fusion partner: (1) the phase 1 flagellin of Salmonella enterica serovar Typhimurium (FliC) doesn't contain any cysteine residue in its molecule, and (2) FliC can be highly expressed as a soluble protein in E. coli. 7 In this study, Pfs25 was expressed in E. coli as a fusion protein with FliC. The Pfs25 gene without signal peptide and GPI-like anchor sequence was firstly PCR amplified from the genomic DNA of P. falciparum 3D7, and then inserted at the downstream of the FliC gene on the pET28a(C)-fliC plasmid (made in our previous study 7 ) (Fig.…”

“…1E). When the recombinant FliC (rFliC) (made in our previous study 7 ) was used as an inhibitor, no such inhibition was appeared. In turn, the mouse immune sera induced by FliC-Pfs25 (made by immunizing BALB/c mice with FliC-Pfs25 formulated with Alum adjuvant) recognized yPfs25 well (Fig.…”

Nasal vaccination using novel mucosal adjuvants : with main focus on influenza A virus

“…The adjuvant has been evaluated together with many different pathogens such as influenza, malaria, HIV and plaque [204,[209][210][211].…”

Pseudomonas aeruginosa flagellin as an adjuvant: superiority of a conjugated form of flagellin versus a mixture with a human immunodeficiency virus type 1 vaccine candidate in the induction of immune responses

“…These changes help DCs process and present antigens more efficiently, stimulating immune responses effectively (Toyota-Hanatani et al, 2008). In addition, recent studies have indicated that flagellin (FliC) is recognized as a pathogen-associated molecular pattern (PAMP) and, as a carrier protein or adjuvant, it is able to increase immune responses to the main antigen, which may be a very small protein or peptide (Qian et al, 2015;Bates et al, 2011). Because of its high immunogenicity and protective effects, bacterial flagellin can be used as a novel candidate for vaccine design (Faezi et al, 2014).…”