Salmonella and Fecal Indicator Bacteria Survival in Soils Amended with Poultry Manure

Hruby, Claire E.; Soupir, Michelle L.; Pederson, Carl H.; Kanwar, Rameshwar S.

doi:10.1007/s11270-017-3667-z

Cited by 44 publications

(31 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Amendment with poultry litter significantly prolonged Salmonella Newport-E survival by 89 days (95% CI = 61.32, 116.02, p ≤ 0.001; Table 2; Figure 4). Overall, these findings are consistent with previous studies (Baloda et al, 2001;Islam et al, 2004a;Holley et al, 2006;You et al, 2006;Nyberg et al, 2010;Ongeng et al, 2011;Hruby et al, 2018;Shah et al, 2019) that reported Salmonella survival increased in soils amended with BSAAO (e.g., poultry litter and bovine manures). For example, You et al (2006) found that addition of bovine manure to agricultural soils increased S. Newport concentrations by as much as 400% within the first 3 days.…”

Section: Salmonella Survived Significantly Longer In Poultry Litter Asupporting

confidence: 92%

“…For example, past studies ( Chandler and Craven, 1980 ; Danyluk et al, 2007 ; Underthun et al, 2018 ; Jechalke et al, 2019 ) have suggested that Salmonella can survive for long periods in soils and survival was dependent on soil characteristics (e.g., moisture, composition, and temperature). Specifically, the use of BSAAOs appears to facilitate Salmonella survival ( Baloda et al, 2001 ; Holley et al, 2006 ; You et al, 2006 ; Nyberg et al, 2010 ; Ongeng et al, 2011 ; Hruby et al, 2018 ; Shah et al, 2019 ). For example, a study conducted in Iowa ( Hruby et al, 2018 ) detected Salmonella in soil samples collected from plots amended with poultry litter approximately 1 year after BSAAO application.…”

Section: Introductionmentioning

confidence: 99%

“…Specifically, the use of BSAAOs appears to facilitate Salmonella survival ( Baloda et al, 2001 ; Holley et al, 2006 ; You et al, 2006 ; Nyberg et al, 2010 ; Ongeng et al, 2011 ; Hruby et al, 2018 ; Shah et al, 2019 ). For example, a study conducted in Iowa ( Hruby et al, 2018 ) detected Salmonella in soil samples collected from plots amended with poultry litter approximately 1 year after BSAAO application. Similarly, a study ( You et al, 2006 ) recovered Salmonella from bovine manure-amended soils 332 days after application in the laboratory (controlled temperature and moisture).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Strain, Soil-Type, Irrigation Regimen, and Poultry Litter Influence Salmonella Survival and Die-off in Agricultural Soils

et al. 2021

View full text Add to dashboard Cite

The use of untreated biological soil amendments of animal origin (BSAAO) have been identified as one potential mechanism for the dissemination and persistence of Salmonella in the produce growing environment. Data on factors influencing Salmonella concentration in amended soils are therefore needed. The objectives here were to (i) compare die-off between 12 Salmonella strains following inoculation in amended soil and (ii) characterize any significant effects associated with soil-type, irrigation regimen, and amendment on Salmonella survival and die-off. Three greenhouse trials were performed using a randomized complete block design. Each strain (~4 log CFU/g) was homogenized with amended or non-amended sandy-loam or clay-loam soil. Salmonella levels were enumerated in 25 g samples 0, 0.167 (4 h), 1, 2, 4, 7, 10, 14, 21, 28, 56, 84, 112, 168, 210, 252, and 336 days post-inoculation (dpi), or until two consecutive samples were enrichment negative. Regression analysis was performed between strain, soil-type, irrigation, and (i) time to last detect (survival) and (ii) concentration at each time-point (die-off rate). Similar effects of strain, irrigation, soil-type, and amendment were identified using the survival and die-off models. Strain explained up to 18% of the variance in survival, and up to 19% of variance in die-off rate. On average Salmonella survived for 129 days in amended soils, however, Salmonella survived, on average, 30 days longer in clay-loam soils than sandy-loam soils [95% Confidence interval (CI) = 45, 15], with survival time ranging from 84 to 210 days for the individual strains during daily irrigation. When strain-specific associations were investigated using regression trees, S. Javiana and S. Saintpaul were found to survive longer in sandy-loam soil, whereas most of the other strains survived longer in clay-loam soil. Salmonella also survived, on average, 128 days longer when irrigated weekly, compared to daily (CI = 101, 154), and 89 days longer in amended soils, than non-amended soils (CI = 61, 116). Overall, this study provides insight into Salmonella survival following contamination of field soils by BSAAO. Specifically, Salmonella survival may be strain-specific as affected by both soil characteristics and management practices. These data can assist in risk assessment and strain selection for use in challenge and validation studies.

show abstract

Section: Salmonella Survived Significantly Longer In Poultry Litter Asupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Strain, Soil-Type, Irrigation Regimen, and Poultry Litter Influence Salmonella Survival and Die-off in Agricultural Soils

et al. 2021

View full text Add to dashboard Cite

show abstract

“…In contrast, the presence of Salmonella on parsley was almost lacking in data obtained during this study. These results coincide with the fact that as microbial content in irrigation source increases, the probability of pathogen's incidence on irrigated crops increases simultaneously (Balkhair ; Hruby et al ).…”

Section: Resultsmentioning

confidence: 99%

Applying Quantitative Microbial Risk Assessment Model in Developing Appropriate Standards for Irrigation Water

Ezzat

2020

Integr Envir Assess & Manag

View full text Add to dashboard Cite

This study aimed to apply a quantitative microbial risk assessment (QMRA) model to estimate the annual risk probability of Salmonella infection associated with the consumption of crops, which were irrigated with raw wastewater abstracted by farmers without official permission. Data generated from the model were used to propose realistic standards for Salmonella in irrigation water needed for safe crop production. Results demonstrated the presence of total coliforms, fecal coliforms, fecal streptococci, and Salmonella in wastewater samples. Salmonella was recorded on lettuce and spinach in 3 different harvesting events, although it was not found in data obtained for parsley plant. The QMRA scenario included surface irrigation, consumption of raw crops, and 1-day withholding period before harvest. The annual risk probability of Salmonella infection for consumers exceeded the target tolerable risk (7.7 × 10 −4 ) for investigated crops. The Pearson's correlation coefficient between different annual risk probabilities showed significant correlation (p < 0.05). The levels of risk posed from spinach and lettuce were roughly similar, although they tended to be higher for lettuce. The optimum concentrations of Salmonella in irrigation water needed to satisfy the World Health Organization guidelines of disease burden (disabilityadjusted life years 10 −6 ) per person per year ranged from 34 to 119 via multiple-tube fermentation technique/100 mL, for spinach and lettuce plants, respectively. The study concluded that unofficial reuse of wastewater in irrigation is a principle route for crop contamination. Water quality monitoring programs should be integrated with QMRA investigations for better estimation of risk level. The fit parameters used could be tailored to cover a wide array of local situations in different countries. Models applied for viruses, helminths, and bacteria other than Salmonella are encouraged in future studies. Integr Environ Assess Manag 2020;16:353-361. © 2019 SETAC

show abstract

“…Therefore, Salmonella can reach agricultural soils via fertilization with pig or poultry manure. Previous reports showed that Salmonella persist in soil for at least 21 days when originating from pig manure (Pornsukarom and Thakur, 2016) and up to one year when originating from poultry manure (Hruby et al, 2018), indicating that soils can act as a long-term Salmonella reservoir. Factors influencing the survival of S. enterica in manure and manure-amended soil were reviewed in Ongeng et al (2015) and include the physical and chemical characteristics of both manure and soil, and also weather and atmospheric conditions, biological interactions, agricultural and livestock management practices, strain variation and physiological age of the cells.…”

Section: Introductionmentioning

confidence: 99%

Salmonella Establishment in Agricultural Soil and Colonization of Crop Plants Depend on Soil Type and Plant Species

et al. 2019

View full text Add to dashboard Cite

Human pathogenic bacteria, such as Salmonella enterica , are able to colonize crop plants. So far, not much is known about biotic and abiotic factors influencing this colonization in field soil. This understanding, however, is imperative for the provision of safe fresh produce to the consumer. In this study, we investigated the effects of soil type, organic fertilization, plant species and the way of Salmonella entry into the plant production system, on the survival of S. enterica in soil as well as the colonization of plants. The selected S. enterica serovar Typhimurium strain 14028s, S. Typhimurium strain LT2 and S. Senftenberg were able to persist in soil for several weeks. Salmonella’s persistence in soil was prolonged in loamy, if compared to sandy soil, and when applied together with organic fertilizer. The leaves of lettuce and corn salad were colonized by S. enterica providing evidence for internalization from the soil via the root. Colonization rates were affected by soil type, plant species and S. enterica strain. Overall, S. enterica was detected in leaves of 0.5–0.9% of the plants, while lettuce was more frequently colonized than corn salad. Plants grown in sandy soil were more often colonized than plants grown in loamy soil. After spray inoculation, S. enterica could be detected on and in leaves for several weeks by cultivation-depending methods, confirmed by confocal microscopy using GFP-labeled S. Typhimurium 14028s. On the one hand, transcriptome data from S. Typhimurium 14028s assessed in response to lettuce medium or lettuce root exudates showed an upregulation of genes associated with biofilm formation and virulence. On the other hand, lettuce inoculated with S. Typhimurium 14028s showed a strong upregulation of genes associated with plant immune response and genes related to stress response. In summary, these results showed that organic fertilizers can increase the persistence of Salmonella in soil and that soil type and plant species play a crucial role in the interactions between human pathogens and crop plants. This understanding is therefore a starting point for new strategies to provide safe food for the consumer.

show abstract

Salmonella and Fecal Indicator Bacteria Survival in Soils Amended with Poultry Manure

Cited by 44 publications

References 63 publications

Strain, Soil-Type, Irrigation Regimen, and Poultry Litter Influence Salmonella Survival and Die-off in Agricultural Soils

Strain, Soil-Type, Irrigation Regimen, and Poultry Litter Influence Salmonella Survival and Die-off in Agricultural Soils

Applying Quantitative Microbial Risk Assessment Model in Developing Appropriate Standards for Irrigation Water

Salmonella Establishment in Agricultural Soil and Colonization of Crop Plants Depend on Soil Type and Plant Species

Contact Info

Product

Resources

About