Salivary lysozyme in smoking alcohol dependent persons

Waszkiewicz, Napoleon; Zalewska-Szajda, Beata; Zalewska, Anna; Waszkiewicz, Magdalena; Szajda, Sławomir Dariusz; Repka, Bernadeta; Szulc, Agata; Kępka, Alina; Minarowska, Alina; Ładny, Jerzy Robert; Zwierz, Krzysztof

doi:10.5603/fhc.2012.0086

Cited by 17 publications

(7 citation statements)

References 6 publications

(19 reference statements)

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“…PBS-lysozyme solution was prepared by dissolving lysozyme (Sigma-Aldrich, Missouri, USA) in PBS at an in vivo concentration (40 mg/L). 37 Peptide 1−4 solutions of 23.2 μM were also prepared with PBS. For each measurement, PBS solution was first loaded into the liquid cell for 5 min to obtain a stable baseline.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

Modification of Titanium Substrates with Chimeric Peptides Comprising Antimicrobial and Titanium-Binding Motifs Connected by Linkers To Inhibit Biofilm Formation

Liu

Duan

et al. 2016

ACS Appl. Mater. Interfaces

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Bacterial adhesion and biofilm formation are the primary causes of implant-associated infection, which is difficult to eliminate and may induce failure in dental implants. Chimeric peptides with both binding and antimicrobial motifs may provide a promising alternative to inhibit biofilm formation on titanium surfaces. In this study, chimeric peptides were designed by connecting an antimicrobial motif (JH8194: KRLFRRWQWRMKKY) with a binding motif (minTBP-1: RKLPDA) directly or via flexible/rigid linkers to modify Ti surfaces. We evaluated the binding behavior of peptides using quartz crystal microbalance (QCM) and atomic force microscopy (AFM) techniques and investigated the effect of the modification of titanium surfaces with these peptides on the bioactivity of Streptococcus gordonii (S. gordonii) and Streptococcus sanguis (S. sanguis). Compared with the flexible linker (GGGGS), the rigid linker (PAPAP) significantly increased the adsorption of the chimeric peptide on titanium surfaces (p < 0.05). Concentration-dependent adsorption is consistent with a single Langmuir model, whereas time-dependent adsorption is in line with a two-domain Langmuir model. Additionally, the chimeric peptide with the rigid linker exhibited more effective antimicrobial ability than the peptide with the flexible linker. This finding was ascribed to the ability of the rigid linker to separate functional domains and reduce their interference to the maximum extent. Consequently, the performance of chimeric peptides with specific titanium-binding motifs and antimicrobial motifs against bacteria can be optimized by the proper selection of linkers. This rational design of chimeric peptides provides a promising alternative to inhibit the formation of biofilms on titanium surfaces with the potential to prevent peri-implantitis and peri-implant mucositis.

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Section: ■ Experimental Sectionmentioning

confidence: 99%

Modification of Titanium Substrates with Chimeric Peptides Comprising Antimicrobial and Titanium-Binding Motifs Connected by Linkers To Inhibit Biofilm Formation

Liu

Duan

et al. 2016

ACS Appl. Mater. Interfaces

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“…The risk of developing CRC increases after the age of 50, and 90 % of cases are registered in people older than this age. In addition to age, the family history, obesity, diabetes, sedentary lifestyle, smoking, high alcohol consumption, eating high-fat, and low-fiber foods are the main risk factors for CRC (Waszkiewicz et al, 2012, Shuwen et al, 2017.…”

Section: Introductionmentioning

confidence: 99%

Relationship Between Salivary and Serum Activity of Beta-Glucuronidase and Multiple Risk Factor for Colorectal Cancer

2020

European Journal of Medicine

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This study is directed to clarify the role of beta-glucuronidase (GUSB) in the occurrence and development of colorectal cancer (CRC). This issue is closely related to life of intestinal microflora, since we know about the beta-glucuronidase of bacterial origin, to play an important role in carcinogenesis along with its own enzyme. The study included the results of determining the specific activity of beta-glucuronidase in the blood serum and saliva. We include to research 24 volunteers who did not have or had from one to four risk factors for CRC. Additionally, we performed the fractionation of blood serum and saliva proteins to establish the possibility of native and bacterial enzyme detection. The study showed the greatest GUSB activity in blood serum and saliva to be a finding in smokers, as well as in the presence of several (three to four) risk factors for CRC. GUSB activity is also correlated with the presence of risk factors such as age and obesity. The peaks of GUSB activity, according to the results of gel chromatography, confirm the fact that bacterial forms contributed to the formation of the overall activity of this enzyme in blood serum and saliva. The obtained results can be used to create diagnostic algorithms aimed at screening for increased risk of CRC in humans based on salivary GUSB analysis.

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“…At present, growing interest in the usability of saliva has been observed [1–4]. Human saliva takes part in the protection against different pathogens of oral tissues and upper respiratory and digestive systems [1, 2].…”

Section: Introductionmentioning

confidence: 99%

“…Also, saliva plays a significant part in the primary phase of digestion and participates in the perception of different kinds of tastes. Moreover, saliva has antibacterial, antifungal, and antiviral properties due to the presence of immunoglobulins, lactoferrin, and lysozyme [4–6].…”

Section: Introductionmentioning

confidence: 99%

Diagnostic Value of Salivary Markers in Neuropsychiatric Disorders

et al. 2019

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A growing interest in the usability of saliva has been observed recently. Using saliva as a diagnostic material is possible because it contains a varied range of composites, organic and inorganic like proteins, carbohydrates, and lipids, which are secreted into saliva. Moreover, this applies to drugs and their metabolites. Saliva collection is noninvasive, and self-collection is possible. There is a lack of risk of injuries related to injection with needle, and it is generally safe. Human saliva has been successfully used, for example, in the diagnosis of many systemic diseases like cancers, autoimmunological diseases, infectious diseases (HIV, hepatitis, and malaria), and endocrinological diseases, as well as diseases of the gastrointestinal tract. Also, it is used in toxicological diagnostics, drug monitoring, and forensic medicine. The usefulness of saliva as a biological marker has also been extended to psychiatry. The specificity of mental illness and patients limits or prevents cooperation and diagnosis. In many cases, the use of saliva as a marker seems to be the most sensible choice.

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Salivary lysozyme in smoking alcohol dependent persons

Cited by 17 publications

References 6 publications

Modification of Titanium Substrates with Chimeric Peptides Comprising Antimicrobial and Titanium-Binding Motifs Connected by Linkers To Inhibit Biofilm Formation

Modification of Titanium Substrates with Chimeric Peptides Comprising Antimicrobial and Titanium-Binding Motifs Connected by Linkers To Inhibit Biofilm Formation

Relationship Between Salivary and Serum Activity of Beta-Glucuronidase and Multiple Risk Factor for Colorectal Cancer

Diagnostic Value of Salivary Markers in Neuropsychiatric Disorders

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