Salivary Glycoconjugates as Biomarkers of Chronic Periodontitis

Sebastian, Roopa; Shivarajashankara, YM; Shantaram, Manjula; Shivashankar, A R; Dayakar, MM

doi:10.14260/jemds/2014/3374

Cited by 3 publications

(3 citation statements)

References 22 publications

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“…In at the present study, Sialic acids levels had a significant association with periodontal health and disease and the levels of sialic acid were elevated in chronic periodontitis subjects. This agreed with studies conducted by Roopa et al, Shiny Inasu et al, Surekha et al and Oktay et al [10][11][12][13] The synthesis of protein or glycoprotein increased with progression and severity of periodontal disease. In addition, the rise in salivary albumin also plays a role in the rise in the total proteins.…”

Section: Discussionsupporting

confidence: 91%

Estimation of salivary glycoconjugates and salivary ros levels in chronic periodontitis: a clinico-biochemical study

Roy¹,

Manjunath²,

Bhat³

et al. 2017

Int J Res Med Sci

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Background: Periodontitis is a chronic inflammatory disease of periodontal tissue, characterized by persistent inflammation of the connective tissue and alveolar bone destruction. Patients with periodontal disease show the differences in the composition of saliva. Newer diagnostic tools based on analysis of body fluids such as saliva, GCF and serum are found to be useful for diagnosis as well as monitoring the disease activity. Thus, aim of the study was to estimate the concentration of salivary glycoconjugates (sialic acid, total protein) and salivary ROS in unstimulated whole saliva of subjects with chronic periodontitis and to compare the concentration with healthy/gingivitis subjects.Methods: The study sample consisted of 60 subjects (33 males and 27 females) with age ranging from 30-60 years. A detailed case history was taken from all the subjects and periodontal disease parameters (bleeding on probing, probing pocket depth and clinical attachment levels) were recorded at baseline and subjects were divided into 2 groups. Group I- control group (healthy/gingivitis subjects) and Group II -test group (chronic periodontitis). Saliva samples were collected from the subjects and stored at -700 ºC. Periodate Resorcinol Assay was done to estimate the levels of sialic acid, Biuret test was done to assess the levels of total protein and d-ROMs test was done to assess the level of ROS. Statistical analysis was done using students unpaired ‘t’ test and Pearsons correlation test. Results: It was found that the levels of salivary glycoconjugates and ROS are increased in subjects with chronic periodontitis when compared to healthy/gingivitis subjects. Thus it can reflect the clinical status of gingival and periodontal tissues.Conclusions: Estimation of the levels of glycoconjugates and ROS may be used as one of the reliable biomarkers to assess the severity of periodontal disease and to monitor the disease progression.

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Section: Discussionsupporting

confidence: 91%

Estimation of salivary glycoconjugates and salivary ros levels in chronic periodontitis: a clinico-biochemical study

Roy¹,

Manjunath²,

Bhat³

et al. 2017

Int J Res Med Sci

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“…A free radical may be defined as an atomic or molecular species capable of independent existence with one or more unpaired electrons in its structures [3]. Reactive oxygen species (ROS) are key signaling molecules that play an important role in the progression of inflammatory disorders [4].…”

Section: Introductionmentioning

confidence: 99%

“…When periodontitis occurs, reactive oxygen species, which are overproduced mostly by hyperactive neutrophils, could not be balanced by antioxidant defense system and cause tissues damage [6]. Patients with periodontitis tend to have lower antioxidant capacity -both locally and systemically [3].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Green Betle Leaf Gel (Piper Betle Leaf) to Total Antioxidant Capacity (TAC) Level after Scaling and Root Planing (SRP) Treatment

Rusminah

Rusyanti

Susanto

et al. 2019

KEM

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Abstract. Green betel leaf gel (Piper betle leaf) has an antimicrobial, antiseptic and antioxidant activity. An imbalance between antioxidants and ROS in the oral cavity have been implicated as one of the progressive or pathogenic factor for periodontal disease. Antimicrobial topical agent delivery may be provided as a supportive therapy for periodontal treatment after scaling and root planing. The purpose of this research is to analyze the effect of green betle leaf gel (Piper betle leaf) to Total Antioxidant Capacity (TAC) level gingival crevicular fluids after scaling and root planing treatment in chronic periodontitis patients. This research is a quasi experimental with pretest and posttest, split mouth. A total of 14 subjects followed this study. The parameters measured were TAC levels gingival crevicular fluids before and after treatment with ELISA examination. Green betel leaf gel was applied to the pocket on the test side after scaling and root planing. Data analysis using Wilcoxon test with p <0.05, and Mann-Whitney test with p <0.05. TAC levels of gingival crevicular fluids increased on day 14 on both sides, on the test side there was a significant increase (p = 0.002) while on the control side the increase was not significant. Green betel leaf gel has an effect to increase TAC level gingival crevicular fluids after scaling and root planing in chronic periodontitis patients.

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The Association between Salivary Metabolites and Gingival Bleeding Score in Healthy Subjects: A Pilot Study

Antonelli,

Ferrari,

Gallo

et al. 2024

IJMS

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Periodontal diseases, including gingivitis and periodontitis, are among the most prevalent diseases in humans. Gingivitis is the mildest form of periodontal disease, characterized by inflammation of the gingiva caused by the accumulation of dental plaque. Salivary diagnostics are becoming increasingly popular due to the variation in saliva composition in response to pathological processes. We used a metabolomics approach to investigate whether a specific saliva metabolic composition could indicate preclinical stage of gingivitis. 1H-NMR spectroscopy was used to obtain the salivary metabolite profiles of 20 healthy subjects. Univariate/multivariate statistical analysis evaluated the whole saliva metabolite composition, and the Full-Mouth Bleeding Score (FMBS) was employed as a classification parameter. Identifying a signature of specific salivary metabolites could distinguish the subjects with high FMBS scores but still within the normal range. This set of metabolites may be due to the enzymatic activities of oral bacteria and be associated with the early stages of gingival inflammation. Although this analysis is to be considered exploratory, it seems feasible to establish an FMBS threshold that distinguishes between the absence and presence of early inflammatory alterations at the salivary level.

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Salivary Glycoconjugates as Biomarkers of Chronic Periodontitis

Cited by 3 publications

References 22 publications

Estimation of salivary glycoconjugates and salivary ros levels in chronic periodontitis: a clinico-biochemical study

Estimation of salivary glycoconjugates and salivary ros levels in chronic periodontitis: a clinico-biochemical study

The Effect of Green Betle Leaf Gel (Piper Betle Leaf) to Total Antioxidant Capacity (TAC) Level after Scaling and Root Planing (SRP) Treatment

The Association between Salivary Metabolites and Gingival Bleeding Score in Healthy Subjects: A Pilot Study

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