Salivary expression of soluble HER2 in breast cancer patients with positive and negative HER2 status

Abstract

BackgroundThe aim of this study was to investigate the relationship between salivary concentration of the soluble fragment of the HER2 (human epidermal growth factor receptor) protein and its status in mammary tissues.MethodsThis case-control study was done in 27 breast cancer patients with no visible metastatic disease treated at the gynecology service, Maternity Souissi Hospital, Rabat, Morocco. Two groups were selected, ie, patients with positive and negative HER2 status in mammary tissue. The salivary HER2 protein concentration was assessed by enzyme-linked immunosorbent assay. The salivary HER2 concentration was compared between the HER2-positive and HER2-negative groups using the Mann-Whitney U test. A P-value <0.05 was considered to be statistically significant.ResultsNo statistically significant difference in salivary HER2 protein expression was found between the case and control groups. There was also no significant difference in clinical characteristics according to positive and negative HER2 status (P>0.05), except for the progesterone hormone receptor which was statistically significant in both the case and control groups (P=0.047).ConclusionAccording to our data, salivary expression of the HER2 receptor may not be a reliable alternative to tissue assessment.

Salivary Biomarkers to Assess Breast Cancer Diagnosis and Progression: Are We There Yet?

“…Her2/neu increased with staging, but not with Her2/neu status. The finding was later supported by other researchers [23,29].…”

Electrochemical Impedance Spectroscopy Characterization of Label-Free Biosensors for the Detection of HER2 in Saliva

“…The biosensor's ability to detect HER2 is the responsiveness of the impedance response toward the increasing concentrations of HER2 in PBS. The concentration range chosen is perfect for the detection of salivary HER2 concentrations in healthy individuals and breast cancer patients alike [16,17]. The resulting fit of the bode plot showed excellent response to the concentrations of HER2, while repeated detection of HER2 have yielded a similarly repeatable curve of Y = (0.0055 ± 0.0003)X + (0.1127 ± 0.0052)\R 2 = 0.9909 with high linearity, indicating high repeatability.…”

“…A solution to the above-mentioned problem is the use a different biological medium for the detection of HER2; this is possible due to the detachability of the extracellular part into the extracellular matrix due to proteolytic cleavage that occur on the cell's surface [8], and while blood is usually the favored medium for the detection of biomarkers, saliva is seen as a great alternative [9][10][11][12]; saliva has several advantages on other biomarker rich biological mediums due to its availability in large quantities, its richness in biomarkers and its ease of collection without needles [13,14]. A few of the hurdles facing salivary detection have unfortunately hindered their use for clinical detection of a large number of diseases; from the low concentration of HER2 within the fM range [15][16][17], to issues and difficulties with the salivary matrix [18]. However, above all, the lack of a technique for the low cost HER2 detection that is optimized for the detection in the salivary matrix [11].…”

Salivary biomarkers for diagnosis of systemic diseases and malignant tumors. A systematic review

“…Molecules most frequently investigated were proteins and RNA. Twelve studies (23.5%) were scored as "good" and 11 of these reported statistically significant associations between molecules searched and pathology (6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17).…”