Salivary Enzyme Levels After Scaling and Interleukin‐1 Genotypes in Japanese Patients With Chronic Periodontitis

Yoshie, Hiromasa; Tai, Hideaki; Kobayashi, Tetsuo; Oda-Gou, Emire; Nomura, Yasuo; Numabe, Yukihiro; Ito, Kazuro; Kurihara, Hidemi; Kamoi, Kazumi

doi:10.1902/jop.2007.060216

Cited by 51 publications

(44 citation statements)

References 25 publications

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“…Statistical analysis comparing between the ALP activity of the three groups of this study revealed a significant difference [P˂ 0.05] between control and gingivitis as the ALP activity increase in gingivitis. This result agree with many results from other studies as in Yoshie et al [24] that showed an increase in the alkaline phosphatase activity in the severe stage of periodontal diseases, and after treatment, the activity of this enzyme was restored to the normal value found in healthy persons. There are some suggestions to explain this increase.…”

Section: Resultssupporting

confidence: 93%

The Effect of Titanium Dioxide Nanoparticles on Salivary Alkaline Phosphatase Activity

Al-Rubaee¹,

Suha²,

Kadim³

2015

European Journal of Molecular Biotechnology

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The structural and optical properties of the titanium dioxide nanoparticles [TiO2 NPs] have been investigated using [UV-Vis] spectrophotometer and SEM. The produced nanoparticles show small and about sharp round peaks around 220 nm. The produced nanoparticles have a spherical shape with an average particle size ˂50 nm. The effect of titanium dioxide NPs was studied on the activity of Alkaline Phosphatase [ALP] in the saliva of 25 patients with gingivitis in comparison to 20 healthy subjects with the average age about 22-23 years for both groups. The results correlated with the observation that salivary alkaline phosphatase activity increase in patient with gingivitis in comparison to control group and salivary ALP activity inhibited by titanium dioxide nanoparticles.

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Section: Resultssupporting

confidence: 93%

The Effect of Titanium Dioxide Nanoparticles on Salivary Alkaline Phosphatase Activity

Al-Rubaee¹,

Suha²,

Kadim³

2015

European Journal of Molecular Biotechnology

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“…1 Alkaline phosphatase (ALP) is an intracellular enzyme present in the saliva and most tissues, organs and bones, including the epithelial, inflammatory cells, bacterial organisms and mineralising tissue cells. [3][4][5] The enzyme is related to cell injury and death. ALP most commonly correlates with bone metabolism and is present in the osteoblast cell membrane and polymorphonuclear leukocyte granules.…”

mentioning

confidence: 99%

“…3 Destructive processes in the alveolar bone can lead to increased ALP activity. 4,5 A key factor in enzyme functionality is pH, with the optimum pH for ALP activity being ≈ 7.4. 6 Therefore, the maximum enzyme activity of ALP is related to the pH and concentration of phosphate esters existing in cells.…”

mentioning

confidence: 99%

Comparison of Salivary pH, Buffering Capacity and Alkaline Phosphatase in Smokers and Healthy Non-Smokers: Retrospective cohort study

Ahmadi‐Motamayel¹,

Falsafi²,

Goodarzi³

et al. 2016

SQUMJ

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abstract:Objectives: Saliva contains alkaline phosphatase (ALP)-a key intracellular enzyme related to destructive processes and cellular damage-and has buffering capacity (BC) against acids due to the presence of bicarbonate and phosphate ions. Smoking may have deleterious effects on the oral environment due to pH changes which can affect ALP activity. This study aimed to evaluate the salivary pH, BC and ALP activity of male smokers and healthy non-smokers. Methods: This retrospective cohort study took place between August 2012 and December 2013. A total of 251 healthy male non-smokers and 259 male smokers from Hamadan, Iran, were selected. Unstimulated whole saliva was collected from each participant and pH and BC were determined using a pH meter. Salivary enzymes were measured by spectrophotometric assay. Results: Mean salivary pH (7.42 ± 0.48 and 7.52 ± 0.43, respectively; P = 0.018) and BC (3.41 ± 0.54 and 4.17 ± 0.71; P = 0.001) was significantly lower in smokers compared to non-smokers. Mean ALP levels were 49.58 ± 23.33 IU/L among smokers and 55.11 ± 27.85 IU/L among non-smokers (P = 0.015). Conclusion: Significantly lower pH, BC and ALP levels were observed among smokers in comparison to a healthy control group. These salivary alterations could potentially be utilised as biochemical markers for the evaluation of oral tissue function and side-effects among smokers. Further longitudinal studies are recommended to evaluate the effects of smoking on salivary components.

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“…Each sample thus collected was centrifuged at 3,000 rpm for 5 min at 4ºC. All the samples were collected in the order described above and then immediately sent to a medical examination laboratory (BML Corporation, Tokyo, Japan) for bacterial analysis (7)(8)(9)12).…”

Section: Sample Collectionmentioning

confidence: 99%

“…Quantitative analysis of periodontal bacteria in clinical specimens, such as saliva, supragingival plaque and subgingival plaque, is important for diagnosis, evaluation, and risk assessment of patients with periodontal disease or who are prone to it. Using real-time PCR methods (5-7), clinicians can easily evaluate these periodontal bacteria and their association with clinical symptoms (8)(9)(10)(11)(12).…”

Section: Introductionmentioning

confidence: 99%

Characterization of specimens obtained by different sampling methods for evaluation of periodontal bacteria

Okada

Sogabe

Takeuchi

et al. 2017

Journal of Oral Science

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Quantitative analysis of periodontal bacteria is considered useful for clinical diagnosis, evaluation and assessment of the risk of periodontal disease. The purpose of this study was to compare the effectiveness of sampling of saliva, supragingival and subgingival plaque for evaluation of periodontal bacteria. From each of 12 subjects, i) subgingival plaque was collected from the deepest pocket using a sterile paper point, ii) stimulated whole saliva was collected after chewing gum, and iii) supragingival plaque was collected using a tooth brush. These samples were sent to the medical examination laboratory for quantitative analysis of the counts of three periodontal bacterial species: Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia. The proportions of these bacteria in subgingival plaque were higher than those in saliva or supragingival plaque, but lower in subgingival plaque than in saliva or supragingival plaque. In several cases, periodontal bacteria were below the levels of detection in subgingival plaque. We concluded that samples taken from subgingival plaque may be more useful for evaluating the proportion of periodontal bacteria in deep pockets than is the case for other samples. Therefore, for evaluation of periodontal bacteria, clinicians should consider the characteristics of the specimens obtained using different sampling methods.

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Salivary Enzyme Levels After Scaling and Interleukin‐1 Genotypes in Japanese Patients With Chronic Periodontitis

Cited by 51 publications

References 25 publications

The Effect of Titanium Dioxide Nanoparticles on Salivary Alkaline Phosphatase Activity

The Effect of Titanium Dioxide Nanoparticles on Salivary Alkaline Phosphatase Activity

Comparison of Salivary pH, Buffering Capacity and Alkaline Phosphatase in Smokers and Healthy Non-Smokers: Retrospective cohort study

Characterization of specimens obtained by different sampling methods for evaluation of periodontal bacteria

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