The Effect of Titanium Dioxide Nanoparticles on Salivary Alkaline Phosphatase Activity

Al-Rubaee, Eaman A; Suha, T.; Kadim, Nadia M.

doi:10.13187/ejmb.2015.10.188

Cited by 4 publications

(4 citation statements)

References 16 publications

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“…The chemical evaluation revealed that TiO2 NPs caused an elevation in peroxidase activity. Al-Rubaee et al in 2016 showed that Total salivary peroxidase was activated significantly (p˂0.001) by TiO2 NPs 13 . The surface of the nanoparticles can cause changes in the form of the protein when it attaches itself to the nanoparticle surface.…”

Section: Discussionmentioning

confidence: 99%

The Effect of titanium dioxide nanoparticles on the activity of salivary peroxidase in periodontitis patients

et al. 2023

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Background:The technology of nanoparticles has been expanded to many aspects of modern life. Titanium dioxide nanoparticles were of many nanomaterials utilized in biomedical applications. The interactions between nanoparticles and proteins are believed to be the base for the biological effect of the nanoparticles. The oxidation reaction of many substances is catalyzed by oxidizing enzymes called peroxidases. The activity of salivary peroxidase is elevated with periodontal diseases. the aim ofthis study is to examine the action of titanium dioxide nanoparticles on salivary peroxidase activity.Material and method75 participants were enrolled in this study—Periodontitis group with 44 participants and the non-periodontitis group with 31 participants. The participants' age range was 35 to 50 years for both groups. The clinical parameters of plaque index, gingival index, probing pocket depth and clinical attachment level were used in this study to determine the presence or absence of the periodontal disease. Unstimulated saliva was collected from all participants and analyzed for the activity of peroxidase enzyme under the effect of titanium dioxide nanoparticles. ResultsThe Periodontitis group showed higher peroxidase enzyme activity than the non-periodontitis group and the activity of salivary peroxidase showed no correlation with the clinical parameters. Titanium dioxide nanoparticles increased salivary peroxidase activity. ConclusionThis study demonstrated that the solid surface of nanoparticles could induce changes in the attached protein molecule which in turn causes changes in the effect of the nanoparticles on living tissue or organism. The titanium dioxide nanoparticles Play a role in increasing the activity of salivary peroxidase within the saliva of chronic periodontitis patients.

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Section: Discussionmentioning

confidence: 99%

The Effect of titanium dioxide nanoparticles on the activity of salivary peroxidase in periodontitis patients

et al. 2023

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“…In another similar study, to determine the effect of TiO 2 nanoparticles on the activity of salivary ALP enzyme in individuals with gingivitis, it was found that the TiO 2 NPs caused inhibition of the salivary ALP activity at lower concentrations more than the higher range of concentrations. 18 In kinetic study on the action of TiO 2 NPs on the enzymatic activity of salivary peroxidase, salivary peroxidase was found to be activated by TiO 2 NPs. 31 A study on the effect of zinc oxide NPs (ZnO NPs) on the enzymatic activity of salivary ALP in chronic periodontitis patients showed that ZnO NPs caused an increase in the salivary ALP activity.…”

Section: Discussionmentioning

confidence: 99%

“…These results agree with the results of other studies that revealed an increase of activity of salivary ALP in patients with chronic periodontitis compared to gingivitis or healthy individuals. [18][19][20] This can be explained as due to the release of the enzyme by bacterial cells and inflammatory cells. 21 ALP is released by secondary granules of neutrophils and its concentration increases greatly with an increase in inflammation.…”

Section: Discussionmentioning

confidence: 99%

Effect of Titanium Dioxide Nanoparticles on the Activity of Salivary Alkaline Phosphatase in Chronic Periodontitis Patients

Ali¹,

Al-Rubaee²,

Talib³

2019

World Journal of Dentistry

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Background: Nanotechnology application has rapidly extended into all areas of life. It provides new methods to solve scientific and medical problems. Nanomaterials such as titanium dioxide nanoparticles (TiO 2 NPs) has been used in biomedical applications. Biological influence of the nanoparticles appears to be as a result of the interactions between the nanoparticles and proteins. Salivary alkaline phosphatase (ALP) is an enzyme that is linked to the outer membrane of some cells and formed by many others. ALP activity increase in periodontal diseases especially in the active phase of the disease.

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“…Other techniques such as naked eyes and ultraviolet–visible spectroscopy (UV-vis) have been used with different materials in detection ALP. For example, AL-Rubaee et al (2015) used UV-vis spectroscopy in a developed platform for ALP detection in saliva-based on titanium dioxide NPs (TiO 2 NPs) [149]. Findings from the study showed that the technique could be applied in sensitive and selective detection of ALPs in patients with gingivitis.…”

Section: Recent Technologymentioning

confidence: 99%

Overview of Optical and Electrochemical Alkaline Phosphatase (ALP) Biosensors: Recent Approaches in Cells Culture Techniques

Balbaied

Moore

2019

Biosensors

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Alkaline phosphatase (ALP), which catalyzes the dephosphorylation process of proteins, nucleic acids, and small molecules, can be found in a variety of tissues (intestine, liver, bone, kidney, and placenta) of almost all living organisms. This enzyme has been extensively used as a biomarker in enzyme immunoassays and molecular biology. ALP is also one of the most commonly assayed enzymes in routine clinical practice. Due to its close relation to a variety of pathological processes, ALP’s abnormal level is an important diagnostic biomarker of many human diseases, such as liver dysfunction, bone diseases, kidney acute injury, and cancer. Therefore, the development of convenient and reliable assay methods for monitoring ALP activity/level is extremely important and valuable, not only for clinical diagnoses but also in the area of biomedical research. This paper comprehensively reviews the strategies of optical and electrochemical detection of ALP and discusses the electrochemical techniques that have been addressed to make them suitable for ALP analysis in cell culture.

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The Effect of Titanium Dioxide Nanoparticles on Salivary Alkaline Phosphatase Activity

Cited by 4 publications

References 16 publications

The Effect of titanium dioxide nanoparticles on the activity of salivary peroxidase in periodontitis patients

The Effect of titanium dioxide nanoparticles on the activity of salivary peroxidase in periodontitis patients

Effect of Titanium Dioxide Nanoparticles on the Activity of Salivary Alkaline Phosphatase in Chronic Periodontitis Patients

Overview of Optical and Electrochemical Alkaline Phosphatase (ALP) Biosensors: Recent Approaches in Cells Culture Techniques

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