Salivary biomarkers in oral squamous cell carcinoma – An insight

Radhika, T; Jeddy, Nadeem; Nithya, S; Muthumeenakshi, R.M.

doi:10.1016/j.jobcr.2016.07.003

Cited by 79 publications

(65 citation statements)

References 20 publications

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“…Saliva has direct contact with OSCC lesions, which gives it particular potential as a specific and sensitive screening tool. While more than 100 potential salivary biomarkers (DNA, RNA, mRNA, miRNA, and protein) have been previously identified for OSCC (reviewed in [5][6][7][8]), further research is required to validate biomarkers for clinical applications.…”

Section: Introductionmentioning

confidence: 99%

Potential Salivary mRNA Biomarkers for Early Detection of Oral Cancer

Kang

et al. 2020

JCM

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We evaluated potential biomarkers in human whole saliva for the early diagnosis of oral squamous cell carcinoma (OSCC). We selected 30 candidate genes with relevance to cancer from recent reports in PubMed. Saliva samples were obtained from 34 non-tumor control and 33 OSCC patients. Real-time PCR was performed, and mRNA levels were compared. Normalized mRNA levels of six genes (NGFI-A binding protein 2 (NAB2), cytochrome P450, family 27, subfamily A, polypeptide 1 (CYP27A1), nuclear pore complex interacting protein family, member B4 (NPIPB4), monoamine oxidase B (MAOB), sialic acid acetyltransferase (SIAE), and collagen, type III, alpha 1 (COL3A1)) were significantly lower in saliva of OSCC patients. Receiver operating characteristics (ROC) analysis was used to individually evaluate the predictive power of the potential biomarkers for OSCC diagnosis. The area under the curve (AUC) values were evaluated for the OSCC vs. non-tumor groups via univariate ROC analyses, as well as multivariate ROC analyses of combinations of multiple potential biomarkers. The combination of CYP27A1 + SIAE showed a favorable AUC value of 0.84. When we divided saliva samples into two groups according to age using a 60-year cut-off, with OSCC patients and controls evaluated together, the AUC of MAOB–NAB2 was more predictive of OSCC in the under-60 group (AUC, 0.91; sensitivity, 0.92; and specificity, 0.86) than any other gene combination. These results are expected to aid the early diagnosis of OSCC, especially in patients under 60 years of age. While more studies with larger numbers of patients are necessary, our result suggest that salivary mRNA would be a potent biomarker for early OSCC diagnosis.

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Section: Introductionmentioning

confidence: 99%

Potential Salivary mRNA Biomarkers for Early Detection of Oral Cancer

Kang

et al. 2020

JCM

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“…Serum biomarkers such as SCCA, TSGF, CEA, and PD-1 have been used as clinical biomarkers for the early diagnosis of many tumors, because they can be detected with high levels of sensitivity and specificity. However, although recent studies showed that serum SCCA was the best available biomarker with a sensitivity of 80% in OSCC patients, its specificity was less than 60% [30-32]. We therefore tested the diagnostic abilities of combinations of these biomarkers for OSCC and showed that the combination of AC007271.3, SCCA, and TSGF discriminated between OSCC and controls with high sensitivity (80.0%) and specificity (93.1%).…”

Section: Discussionmentioning

confidence: 96%

SCCA, TSGF, and the Long Non-Coding RNA AC007271.3 are Effective Biomarkers for Diagnosing Oral Squamous Cell Carcinoma

Shao¹,

Huang²,

Zheng³

et al. 2018

Cell Physiol Biochem

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Background/Aims: Oral squamous cell carcinoma (OSCC) is one of the most lethal malignancies worldwide and the most common type of oral cancer, characterized by invasive growth, frequent regional metastases, high recurrence, and poor prognosis. In the current study, we investigated the use of long non-coding RNAs (lncRNAs), tumor-specific growth factor (TSGF), and squamous cell carcinoma antigen (SCCA) as potential biomarkers for OSCC screening. Methods: LncRNA expression was measured by microarray analysis in three sets of OSCC and paired normal mucosal tissues. The potential lncRNAs involved in OSCC development were investigated by bioinformatics and verification experiments. We also determined the expression of these potential biomarkers in tissue and serum samples in a case–control study of 80 OSCC cases and 70 controls. Receiver operating characteristics, decision curve analysis, and the combined detection of lncRNA AC007271.3, TSGF, and SCCA were carried out to screen for OSCC biomarkers. Results: A total of 691 lncRNAs (433 upregulated and 258 downregulated) were differentially expressed in OSCC tissues compared with normal controls (p< 0.05). Based on Gene Ontology and pathway analysis, we selected four differentially expressed lncRNAs (AC007271.3, AC007182.6, LOC283481, and RP11-893F2.9), and showed that aberrant AC007271.3 levels in OSCC patients were significantly associated with clinical stage, especially in early-stage disease, in an expanded case–control study. The combination of AC007271.3 and SCCA (AUC=0.902, p< 0.001) showed significantly better ability to discriminate between OSCC and controls compared with SCCA or AC007271.3 alone. Serum AC007271.3, SCCA, and TSGF levels could also discriminate between OSCC and normal controls with sensitivities of 77.6%, 55.0%, and 63.3%, and specificities of 84.5%, 93.3%, and 66.7%, respectively. Conclusions: These results suggest that AC007271.3, SCCA, and TSGF could be novel circulating biomarkers for the determination of OSCC. However, further validation in large-scale prospective studies is necessary.

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“…Biomarkers have been classified based on biomolecules and disease states [74,75]. Based on biological molecules: .…”

Section: Classification Of Salivary Biomarkers For Oral Cancermentioning

confidence: 99%

Salivary Diagnostics in Oral Diseases

Bhat¹,

Bhat²

2019

Saliva and Salivary Diagnostics

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Common oral diseases like dental caries, periodontal diseases and oral cancer have major impact on quality of life. For prevention, treatment and prognosis, it is essential to measure the disease objectively and accurately in a quantitative manner. Quantification of biochemical or molecular specific products of cancers in serum or localized body juices can be one of the current methods of measuring oral diseases objectively. Salivary diagnostics has influenced several researchers and has been verified as an important tool in the diagnosis of many systemic conditions and prognosis of the disease. Developments in the field of molecular biology, salivary genomics and proteomics have directed to the detection of novel molecular markers for oral disease diagnosis, therapeutics and prognosis.

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Salivary biomarkers in oral squamous cell carcinoma – An insight

Cited by 79 publications

References 20 publications

Potential Salivary mRNA Biomarkers for Early Detection of Oral Cancer

Potential Salivary mRNA Biomarkers for Early Detection of Oral Cancer

SCCA, TSGF, and the Long Non-Coding RNA AC007271.3 are Effective Biomarkers for Diagnosing Oral Squamous Cell Carcinoma

Salivary Diagnostics in Oral Diseases

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