Salivary biomarkers in association with periodontal parameters and the periodontitis risk haplotype

Abstract

Genetic factors play a role in periodontitis. Here we examined whether the risk haplotype of MHC class III region BAT1-NFKBIL1-LTA and lymphotoxin-α polymorphisms associate with salivary biomarkers of periodontal disease. A total of 455 individuals with detailed clinical and radiographic periodontal health data were included in the study. A 610 K genotyping chip and a Sequenom platform were used in genotyping analyses. Phospholipid transfer protein activity, concentrations of lymphotoxin-α, IL-8 and myeloperoxidase, and a cumulative risk score (combining Porphyromonas gingivalis, IL-1β and matrix metalloproteinase-8) were examined in saliva samples. Elevated IL-8 and myeloperoxidase concentrations and cumulative risk scores associated with advanced tooth loss, deepened periodontal pockets and signs of periodontal inflammation. In multiple logistic regression models adjusted for periodontal parameters and risk factors, myeloperoxidase concentration (odds ratio (OR); 1.37, P = 0.007) associated with increased odds for having the risk haplotype and lymphotoxin-α concentration with its genetic variants rs2857708, rs2009658 and rs2844482. In conclusion, salivary levels of IL-8, myeloperoxidase and cumulative risk scores associate with periodontal inflammation and tissue destruction, while those of myeloperoxidase and lymphotoxin-α associate with genetic factors as well.

Salivary changes in oxidative stress related to inflammation in oral and gastrointestinal diseases

“…Several studies have found higher levels of faecal LF in patients with CD (Borkowska et al., 2015; Wright et al., 2016), but there is no evidence regarding LF in patients with OFG. Salivary concentrations of MPO were not influenced by the chronic inflammatory conditions present in the mouth or intestine, which contrasts with the raised levels of salivary MPO activity associated with chronic or aggressive periodontitis and increased plasma MPO concentrations associated with other acute conditions (Borges et al., 2009; Liukkonen et al., 2018).…”

Periodontitis association with IL-8 gene polymorphisms

“…A study done by Liukkone et al included 455 participants. A clinical examination was performed by two periodontologists, and 3 groups were formed: BOP (bleeding on probing), pocket depth, alveolar bone loss, and each group had flow cytometry done for the IL-8 concentration measurements 16 . Research results have shown elevated results for patients who had BOP >25%, a higher number of teeth, or deeper gingiva pockets, which concludes that IL-8 concentration has association with periodontal tissues inflammation and tissue destruction 16 .…”

“…Two studies were done with periodontitis affected patients, analysing IL-8 polymorphisms association with the disease 16,19 . Two studies analysed periodontitis and type 1 or 2 diabetes mellitus association with IL-8 polymorphisms along with patients affected by periodontal pathologies 17,18 .…”

Relación de la periodontitis y artritis reumatoide a través del eje IL-23/IL-17A

“…38,39 eje Il-23/Il-17a en perIodontItIs La IL-23 y la IL-17 han sido evaluadas en distintas muestras biológicas como líquido crevicular gingival (LCG), saliva, suero y plasma, en donde la IL-23 se ha reportado elevada en el LCG de pacientes con periodontitis, 40,41 así como en saliva. 42 Al contrario de Sadeghi R y colaboradores, quienes encontraron disminuida la IL-23 en suero al compararla con los sujetos sanos. 43 Por otra parte, Shimada Y y colegas no observaron diferencias significativas de IL-23 entre sujetos sanos y periodontitis en muestras de LCG.…”