Saliva RT-PCR Sensitivity Over the Course of SARS-CoV-2 Infection

Wyllie, Anne L.; Premsrirut, Prem K.

doi:10.1001/jama.2021.21703

Cited by 6 publications

(2 citation statements)

References 7 publications

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“…The advantages of a saliva sample (SS) compared to NPS for SARS-CoV-2 diagnosis are clearly indicated by the non-invasive collection method, reduced need for healthcare professional handling, and the possibility of self-collection and sampling, even outside the hospital. Several studies indicated that saliva sampling might be considered an alternative tool for the detection of SARS-CoV-2 sequences (Fan et al, 2021 ; Yee et al, 2021 ; Wyllie and Premsrirut, 2022 ), and some reports showed similar performances in SARS-CoV-2-specific multiplex RT-PCR when NPS and SS were compared (Ana Laura et al, 2021 ; Banerjee et al, 2021 ; Yee et al, 2021 ). SS may be very useful in pediatric patients since NPS has proven to be difficult to perform in children, especially those in the infant age.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of saliva and nasopharyngeal swab sampling for genomic detection of SARS-CoV-2 in children accessing a pediatric emergency department during the second pandemic wave

et al. 2023

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SARS-CoV-2 infection is mainly detected by multiplex real-time RT-PCR from upper respiratory specimens, which is considered the gold-standard technique for SARS-CoV-2 infection diagnosis. A nasopharyngeal (NP) swab represents the clinical sample of choice, but NP swabbing can be uncomfortable to the patients, especially for pediatric-age participants, requires trained healthcare personnel, and may generate an aerosol, increasing the intrinsic exposure risk of healthcare workers. The objective of this study was to compare paired NP and saliva samples (SS) collected from pediatric patients to evaluate whether the saliva collection procedure may be considered a valuable alternative to the classical NP swab (NPS) sampling in children. In this study, we describe a SARS-CoV-2 multiplex real-time RT-PCR protocol for SS, comparing the results with the paired NPS specimens from 256 pediatric patients (mean age 4.24 ± 4.40 years) admitted to the hospital emergency room of Azienda Ospedaliera Universitaria Integrata (AOUI), Verona, and randomly enrolled between September 2020 and December 2020. The saliva sampling demonstrated consistent results when compared to NPS use. The SARS-CoV-2 genome was detected in 16 out of 256 (6.25%) NP samples, among which 13 (5.07%) were positive even when paired SS were analyzed. Moreover, SARS-CoV-2-negative NPS and SS were consistent, and the overall concordances between NPS and SS were detected in 253 out of 256 samples (98.83%). Our results suggest that saliva samples may be considered a valuable alternative to NPS for SARS-CoV-2 direct diagnosis with multiplex real-time RT-PCR in pediatric patients.

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Section: Introductionmentioning

confidence: 99%

Evaluation of saliva and nasopharyngeal swab sampling for genomic detection of SARS-CoV-2 in children accessing a pediatric emergency department during the second pandemic wave

et al. 2023

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“…Thus, the fluorophore moves away from the quencher, resulting in an increased signal. Although it has been used to identify SARS-CoV-2 infection [18] , [19] , [20] , [21] , some notable disadvantages still remain. The TaqMan probes often contain 25- to 35-nucleotides to ensure their binding specificity [22] .…”

Section: Introductionmentioning

confidence: 99%

Dumbbell-type triplex molecular switch-based logic molecular assays of SARS-CoV-2

Chen

Liu²,

Wang³

et al. 2022

Sensors and Actuators B: Chemical

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The impact of SARS-CoV-2 variants on the likelihood of children identified as sources of infection in the NIH workforce: a cohort study

van Loben Sels,

Bailin,

Bell

et al. 2023

Preprint

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BackgroundChildren (<18 years old) were not initially considered significant sources of infection (SOIs) for SARS-CoV-2. Risk mitigation strategies were thus prioritized for adults, and vaccination was inaccessible for children until mid-2021. Emergence of novel variants led to significant increases in COVID-19 cases in both children and adults. Whether these emergence events and increased vulnerability of unvaccinated children had a synergistic effect resulting in increased caseloads in adults requires further exploration.MethodsA retrospective cohort study was conducted among 3,545 workers diagnosed with COVID-19. Case details were compiled during contact investigations. Variants of concern were identified following sequencing of biological samples collected through employer-based testing programs. Logistic regression was performed to compare the odds of having a child SOI based on the dominant variant in the workforce.ResultsOne-fourth (24.5%) of the cohort reported having a child in-residence; 11.2% identified a child as their SOI. In Alpha-dominant months, the odds of having a child SOI were 0.3, and the child SOI was likely older (5-17 years old). The odds of having a child SOI increased to 1.3 and 2.2 in Delta- and Omicron-dominant months, respectively. The odds of having younger child SOIs (<5 years old) were significantly higher in Omicron-dominant months.ConclusionsChildren were highly likely to acquire the virus and posed a significant risk of transmission to their adult caretakers during Delta- and Omicron-dominant months. Without proper mitigation strategies in both the home and the workplace, child-associated transmission can threaten operations in the forms of staff shortages.What is already known on this topicIncreases in transmission trends related to SARs-CoV-2 Variants of Concern have been documented in the literature at the population level and in workplaces.What this study addsThis study looks more closely at the role that children played in transmission to adult workers, and therefore their potential to seed transmission outside of the home. This interface of transmission has been neglected in the literature but is key for future policy development.How this study might affect research, practice, or policyTransmission of SARS-CoV-2 from children to their caretakers may cause significantly increased odds of infection in a worker population. This may have second order effects for staffing, particularly in workgroups with employees of childbearing age. Employers should consider this in the design of their policies for continuity of operations, telework, and leave.

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Saliva RT-PCR Sensitivity Over the Course of SARS-CoV-2 Infection

Cited by 6 publications

References 7 publications

Evaluation of saliva and nasopharyngeal swab sampling for genomic detection of SARS-CoV-2 in children accessing a pediatric emergency department during the second pandemic wave

Evaluation of saliva and nasopharyngeal swab sampling for genomic detection of SARS-CoV-2 in children accessing a pediatric emergency department during the second pandemic wave

Dumbbell-type triplex molecular switch-based logic molecular assays of SARS-CoV-2

The impact of SARS-CoV-2 variants on the likelihood of children identified as sources of infection in the NIH workforce: a cohort study

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