Saliva electrophoretic protein profiles in infants: Changes with age and impact of teeth eruption and diet transition

Analysis of Salivary Proteome in Patients with Dental Caries employing Electrophoresis and Mass Spectrometry

“…The authors described electrophoretic differences to the carbohydrate moiety of the enzyme, although a thorough study of 140 isoforms of alphaamylase in human saliva concluded that glycosylation alone cannot account for the multiplicity of amylase spots covering the 18 to 59 kDa range. 15 The Ig alpha-2 chain C region was detected as band B2. The IgG, IgM, IgA, and secretory IgA (slgA) form the basis of the specific salivary defense against oral microbial flora, including mutans streptococci.…”

“…Despite a large number of studies devoted to the characterization of the dense-core granules, information about their composition remains limited. 6 To compare the MS analysis of HWS, the study by Morzel et al 15 analyzed samples of 3-and 6-month-old infants and isolated 21 bands up to 11 kDa on silver staining, while we have identified 9 bands on PAS staining up to 25 kDa. They noted two serum albumin bands at 38.9 and 48.9 kDa, similarly we located two serum albumin bands at approximately 55 and 43 kDa.…”

Proteomic characterization of the acid-insoluble fraction of whole saliva from preterm human newborns

“…Acidic treatment causes precipitation of several salivary proteins. In previous studies, our group was engaged in the proteomic characterization of the acidic soluble components of human saliva collected from donors of various ages [6,8,9,11].…”

“…Very few studies were focused on determining the proteome composition of infant saliva, i.e. Manconi et al examined the age range 0-48 months by a top-down platform based on RP-HPLC-ESI-MS [5], Morzel et al the age range 3-6 months by SDS-PAGE coupled to MALDI-TOF/TOF MS [6], and in both cases major changes in the salivary protein composition in early childhood were evidenced.…”

Multi-omics profiling reveals that eating difficulties developed consecutively to artificial nutrition in the neonatal period are associated to specific saliva composition

“…At a population level for example, individuals from population with high-starch diets have more copies of the salivary amylase gene AMY1 than those from populations with low-starch diets [26]. At an individual level, shift from a mixed to a lactovegetarian diet modified some salivary characteristics such as its sodium content or buffering capacity [27], while change from a milkbased to a diversified diet in infants had an impact both on salivary protein [28] and peptide [29] profiles. Another study compared two groups of subjects, controls or subjected to a dietary intervention from infancy, who were the followed for up to 16 years of age.…”