Saliva application in oral and systemic diseases

Yordanova, Marinela; Gerova, Daniela; Galunska, Bistra

doi:10.14748/ssm.v50i2.4760

Cited by 3 publications

(4 citation statements)

References 23 publications

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“…Saliva was obtained from the cotton pad by centrifugation and kept frozen till the time of analysis of MMP-8. 19 Laboratory assessment: Salivary matrix metalloproteinase was assayed by ELISA supplied by Bioassay Technology (China). Statistical Analysis: The collected data was revised, coded, tabulated, and introduced to a PC using the statistical package for Social Science (IBM Corp.…”

Section: Methodsmentioning

confidence: 99%

Effect of Locally Injectable Platelet-Rich Plasma as an Adjunct to Scaling and Root Planing on Salivary MMP-8 Level in Periodontitis Patients

2023

Mansoura Journal of Dentistry

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Objective: To assess the effect of platelet-rich plasma (PRP) local injection in the treatment of periodontitis, as an adjunct to scaling and root planing (SRP).Materials and Methods: Ninety subjects were involved in this study. They were divided into 3 groups. Group I (negative control) included 30 subjects with healthy periodontium. Group II (positive control) included 30 periodontit is patients who were treated by SRP planing. Group III (study) included 30 periodontitis patients who were treated with SRP and PRP injections. Plaque index (PI), Gingival index (GI), probing pocket depth (PPD), and clinical attachment level (CAL) in addition to MMP-8 level recorded at baseline for all groups and 2 months post-treatment for groups II and III .Results: At baseline; PI, GI, PPD, CAL, and MMP-8 revealed no significant difference between group II and III (p>0.05), while the periodontal parameters of both groups showed highly significantly higher values versus group I (p<0.001 for each). Both groups showed a highly significant decrease in PI, GI, PPD, and CAL after treatment (p<0.001 for each). In addition, Group III showed significantly lower values in PI (P=0.028) and GI (p=0.032) and a highly significant decrease in PPD and CAL (p<0.001 for each) versus Group II. However, after treatment, both groups II and III showed a highly significant decrease in MMP-8 (p<0.001 and p=0.010 respectively). No significant difference was found in the MMP-8 level between Group II and III as the mean±SD was 1.73±0.58 and 1.9±0.49 respectively. Conclusions: Injection of autogenous PRP in addition to SRP in periodontal pockets can enhance clinical parameters of periodontitis.

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Section: Methodsmentioning

confidence: 99%

Effect of Locally Injectable Platelet-Rich Plasma as an Adjunct to Scaling and Root Planing on Salivary MMP-8 Level in Periodontitis Patients

2023

Mansoura Journal of Dentistry

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“…used today, studies on saliva analysis as a supportive tool are continuing. The level of C-reactive protein (CRP) in saliva can be determined by using a system based on nano-biochips, that can be applied chair-side (5). In a study of acute myocardial infarction patients who experi-enced chest pain in the previous 48 hours, CRP, MMP-9, IL-1β, adiponectin, gro-α, E-selectin, and IL-18 increased in saliva more than serum (42).…”

Section: Systemic Diseases Cardiovascular Diseasesmentioning

confidence: 99%

“…Organic substances are composed of urea, uric acid, glucose, fatty acids, glycerides, creatinine, peroxidase, amylase, and hormones. As for the inorganic molecules; Na + , K + , Ca 2+ , Cl − , Mg 2+ , F − , I − , HCO 3 -and H 2 PO 4 are some of the ions found in saliva (4,5). These substances in the structure of saliva have different functions (6).…”

Section: Introductionmentioning

confidence: 99%

Saliva as a Diagnostic Tool in Oral Diseases

Kart¹,

Yarat²

2020

Experimed

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Saliva is a body fluid, which is secreted from 3 major salivary glands (parotid, submandibular and sublingual) and many minor salivary glands. It contains organic and inorganic substances with 99% water, and has several functions such as contribution to articulation, digestion, cleansing and protection of the oral mucosa, and antimicrobial effects. The concentration of saliva's substances vary according to the responses to physiological processes in the human body. Containing genetic materials such as DNA and RNA also highlights saliva as a potential diagnostic tool. Saliva analysis has advantages such as being cheaper, non-invasive, easier patient cooperation, and low technical sensitivity compared to blood tests; it can be used in the diagnosis of many diseases or routine risk assessments. It is also promising for its advantages in collection and storage of the samples. Advances in molecular biology, genomics, and proteomics have revealed the importance of saliva in the detection of many diseases, coining the term salivaomics. The early diagnosis of the diseases in which symptoms can be seen in late stages, may provide an easier treatment and improve the prognosis. In this review, the biomarkers of saliva in the presence of different diseases and the advantages of their use in diagnosis were examined.

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“…A solution to the above-mentioned problem is the use a different biological medium for the detection of HER2; this is possible due to the detachability of the extracellular part into the extracellular matrix due to proteolytic cleavage that occur on the cell's surface [8], and while blood is usually the favored medium for the detection of biomarkers, saliva is seen as a great alternative [9][10][11][12]; saliva has several advantages on other biomarker rich biological mediums due to its availability in large quantities, its richness in biomarkers and its ease of collection without needles [13,14]. A few of the hurdles facing salivary detection have unfortunately hindered their use for clinical detection of a large number of diseases; from the low concentration of HER2 within the fM range [15][16][17], to issues and difficulties with the salivary matrix [18].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Impedance Spectroscopy Characterization of Label-Free Biosensors for the Detection of HER2 in Saliva

Nemeir

Mouawad

Saab

et al. 2020

The 1st International Electronic Conference on Biosensors

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Breast cancer is the leading cancer type for women with two million new yearly infections and more than half a million dead worldwide. Human Epidermal Receptor 2 (HER2) is a prominent breast cancer biomarker that indicates aggressive cancer and is often associated with a bad prognosis and low survival rates. However, current detection methods for HER2 are often time-consuming, expensive, and require a high level of expertise. Biosensors are devices that turn biological interaction into a readable electronic signal; they are known for their high specificity and selectivity for low concentration, as well as their low cost and ease of use, thus making them a better alternative to traditional methods. Also, saliva is becoming a better alternative to blood for the detection of biomarkers due to its non-invasive collection in large quantities with simple collection methods with a richness in disease biomarkers including HER2. Thus, this project aims to develop a label-free, low cost, electrochemical biosensor for the detection of HER2 in saliva. This was done by first depositing diazonium salt onto a screen-printed electrode (SPE) through cyclic voltammetry, then immobilizing anti-HER2 antibodies on the activated SPE using 1-ethyl-3-(3-dimethylamino) propyl carbodiimide/N-hydroxysuccinimide. HER2 biomarker concentrations were detected using electrochemical impedance spectroscopy inside a microfluidic system. The biosensor showed a higher linear detection of HER2 (Y = 0.0062X + 0.1066/R2 = 0.9909) in its physiological concentration range of 5 and 40 pg/mL when compared to other interference proteins: Epidermal Growth Factor Receptor (Y = 0.0016X + 0.0188/R2 = 0.8072) and Human Epidermal Receptor 3 (Y = (0.0035X + 0.0225/R2 = 0.1302). The biosensor was then used to detect 10 pg/mL of HER2 concentration in real saliva using the standard addition methods (Y = 0.0118X + 0.1282/R2 = 0.9876).

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Saliva application in oral and systemic diseases

Cited by 3 publications

References 23 publications

Effect of Locally Injectable Platelet-Rich Plasma as an Adjunct to Scaling and Root Planing on Salivary MMP-8 Level in Periodontitis Patients

Effect of Locally Injectable Platelet-Rich Plasma as an Adjunct to Scaling and Root Planing on Salivary MMP-8 Level in Periodontitis Patients

Saliva as a Diagnostic Tool in Oral Diseases

Electrochemical Impedance Spectroscopy Characterization of Label-Free Biosensors for the Detection of HER2 in Saliva

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